Coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function
Abstract: Introduction: The present study was designed to evaluate the therapeutic efficacy of melatonin and insulin coadministration in diabetes-induced renal injury in rats. Research Design and Methods: Diabetes was achieved by giving streptozotocin (15 mg/kg) for 6 consecutive days. The diabet...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Karger
2022
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| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/13640 |
| Aporte de: |
| id |
I33-R139123456789-13640 |
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| record_format |
dspace |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
CITOQUINAS INSULINA MELATONINA ESTRES OXIDATIVO CORTEZA RENAL NEUROENDOCRINOLOGÍA |
| spellingShingle |
CITOQUINAS INSULINA MELATONINA ESTRES OXIDATIVO CORTEZA RENAL NEUROENDOCRINOLOGÍA Hajam, Younis Ahmad Rai, Seema Pandi Perumal, Seithikurippu R. Brown, Gregory M. Reiter, R. Cardinali, Daniel Pedro Coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function |
| topic_facet |
CITOQUINAS INSULINA MELATONINA ESTRES OXIDATIVO CORTEZA RENAL NEUROENDOCRINOLOGÍA |
| description |
Abstract: Introduction: The present study was designed to evaluate
the therapeutic efficacy of melatonin and insulin coadministration
in diabetes-induced renal injury in rats. Research Design and Methods: Diabetes was achieved by giving streptozotocin
(15 mg/kg) for 6 consecutive days. The diabetic
condition was confirmed by assessing the blood glucose level;
animals having blood glucose levels above 250 mg were
considered as diabetic. Following the confirmation, animals
were randomly divided into different experimental groups,
viz group I served as the control (CON), group II diabetic (D),
group III D+melatonin (MEL), group IV D+insulin (INS), group
V D+MEL+INS, group VI D+glibenclamide (GB), group VII
CON+MEL, group VIII CON+INS, and group IX CON+GB. Following
the completion of the experimental period, animals
were sacrificed, blood was collected via a retro-orbital puncture,
and kidneys were harvested. Diabetic rats exhibited a
significant increment in blood glucose and biochemical indexes
of renal injury (tubular disruption, swollen glomeruli
with loss of glomerular spaces, and distortion of the endothelial
lining) including augmented levels of serum creatinine,
urea, uric acid, Na+, and K+, and inhibition/suppression
of the activity of glutathione (GSH) peroxidase, GSH reductase,
glucose-6-phosphate dehydrogenase, and GSH-Stransferase
in the renal cortex. Results: By examining thiobarbiturate
reactive substances, reduced GSH, superoxide
dismutase activity, and catalase activity in the renal cortex of
control and diabetic rats, it was documented that treatment
with melatonin or insulin alone or in combination showed a
significant ad integrum recovery of GSH-dependent antioxidative
enzymatic activities. Melatonin and insulin coadministration
caused greater reductions in circulating tumor necrosis
factor-α, tumor growth factor-β1, interleukin (IL)-1β,
and IL-6 levels in diabetic rats, whereas IL-10 levels increased,
as compared to each treatment alone. Diabetic rats showed
a significant increase in the expression of both MT1 and MT2
melatonin receptor genes. Melatonin or insulin treatment
alone or in combination resulted in significant restoration of
the relative expression of both melatonin receptors in the
renal cortex. Conclusion: The coadministration of exogenous
melatonin and insulin abolished many of the deleterious
effects of type 1 diabetes on rat renal function. |
| format |
Artículo |
| author |
Hajam, Younis Ahmad Rai, Seema Pandi Perumal, Seithikurippu R. Brown, Gregory M. Reiter, R. Cardinali, Daniel Pedro |
| author_facet |
Hajam, Younis Ahmad Rai, Seema Pandi Perumal, Seithikurippu R. Brown, Gregory M. Reiter, R. Cardinali, Daniel Pedro |
| author_sort |
Hajam, Younis Ahmad |
| title |
Coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function |
| title_short |
Coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function |
| title_full |
Coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function |
| title_fullStr |
Coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function |
| title_full_unstemmed |
Coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function |
| title_sort |
coadministration of melatonin and insulin improves diabetes-induced impairment of rat kidney function |
| publisher |
Karger |
| publishDate |
2022 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/13640 |
| work_keys_str_mv |
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