Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model

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Abstract: Background: Toxoplasmosis is caused by the parasite Toxoplasma gondii that can infect the central nervous system (CNS), promoting neuroinflammation, neuronal loss, neurotransmitter imbalance and behavioral alterations. T. gondii infection is also related to neuropsychiatric disorders suc...

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Autores principales: Leite, Paulo Emilio Correa, Araujo Portes, Juliana de, Rodrigues Pereira, Mariana, Baldino Russo, Fabiele, Martins-Duarte, Erica S., Almeida, Nathalia dos Santos, Attias, Marcia, Barrantes, Francisco José, Baleeiro Beltrão-Braga, Patricia Cristina, Souza, Wanderley de
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2021
Materias:
TOXOPLASMA GONDII
TRASTORNOS NEUROPSIQUIATRICOS
INFLAMACION
CEREBRO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/12526
https://doi.org/10.1016/j.bbih.2020.100190
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-12526
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic TOXOPLASMA GONDII
TRASTORNOS NEUROPSIQUIATRICOS
INFLAMACION
CEREBRO
spellingShingle TOXOPLASMA GONDII
TRASTORNOS NEUROPSIQUIATRICOS
INFLAMACION
CEREBRO
Leite, Paulo Emilio Correa
Araujo Portes, Juliana de
Rodrigues Pereira, Mariana
Baldino Russo, Fabiele
Martins-Duarte, Erica S.
Almeida, Nathalia dos Santos
Attias, Marcia
Barrantes, Francisco José
Baleeiro Beltrão-Braga, Patricia Cristina
Souza, Wanderley de
Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model
topic_facet TOXOPLASMA GONDII
TRASTORNOS NEUROPSIQUIATRICOS
INFLAMACION
CEREBRO
description Abstract: Background: Toxoplasmosis is caused by the parasite Toxoplasma gondii that can infect the central nervous system (CNS), promoting neuroinflammation, neuronal loss, neurotransmitter imbalance and behavioral alterations. T. gondii infection is also related to neuropsychiatric disorders such as schizophrenia. The pathogenicity and inflammatory response in rodents are different to the case of humans, compromising the correlation between the behavioral alterations and physiological modifications observed in the disease. In the present work we used BrainSpheres, a 3D CNS model derived from human pluripotent stem cells (iPSC), to investigate the morphological and biochemical repercussions of T. gondii infection in human neural cells. Methods: We evaluated T. gondii ME49 strain proliferation and cyst formation in both 2D cultured human neural cells and BrainSpheres. Aspects of cell morphology, ultrastructure, viability, gene expression of neural phenotype markers, as well as secretion of inflammatory mediators were evaluated for 2 and 4 weeks post infection in BrainSpheres. Results: T. gondii can infect BrainSpheres, proliferating and inducing cysts formation, neural cell death, alteration in neural gene expression and triggering the release of several inflammatory mediators. Conclusions: BrainSpheres reproduce many aspects of T. gondii infection in human CNS, constituting a useful model to study the neurotoxicity and neuroinflammation mediated by the parasite. In addition, these data could be important for future studies aiming at better understanding possible correlations between psychiatric disorders and human CNS infection with T. gondii.
format Artículo
author Leite, Paulo Emilio Correa
Araujo Portes, Juliana de
Rodrigues Pereira, Mariana
Baldino Russo, Fabiele
Martins-Duarte, Erica S.
Almeida, Nathalia dos Santos
Attias, Marcia
Barrantes, Francisco José
Baleeiro Beltrão-Braga, Patricia Cristina
Souza, Wanderley de
author_facet Leite, Paulo Emilio Correa
Araujo Portes, Juliana de
Rodrigues Pereira, Mariana
Baldino Russo, Fabiele
Martins-Duarte, Erica S.
Almeida, Nathalia dos Santos
Attias, Marcia
Barrantes, Francisco José
Baleeiro Beltrão-Braga, Patricia Cristina
Souza, Wanderley de
author_sort Leite, Paulo Emilio Correa
title Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model
title_short Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model
title_full Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model
title_fullStr Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model
title_full_unstemmed Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model
title_sort morphological and biochemical repercussions of toxoplasma gondii infection in a 3d human brain neurospheres model
publisher Elsevier
publishDate 2021
url https://repositorio.uca.edu.ar/handle/123456789/12526
https://doi.org/10.1016/j.bbih.2020.100190
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