Lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons

Abstract: Neuronal 7 and 4 2 are the predominant nicotinic acetylcholine receptor (nAChR) subtypes found in the brain, particularly in the hippocampus. The e ects of lovastatin, an inhibitor of cholesterol biosynthesis, on these two nAChRs endogenously expressed in rat hippocampal neuronal cell...

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Autores principales: Borroni, María Virginia, Kamerbeek, Constanza B., Pediconi, María F., Barrantes, Francisco José
Formato: Artículo
Lenguaje:Inglés
Publicado: MDPI 2020
Materias:
MEDICINA
COLESTEROL
RECEPTORES
ENFERMEDAD DE ALZHEIMER
NEURONAS
HIPOCAMPO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/10847
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-10847
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic MEDICINA
COLESTEROL
RECEPTORES
ENFERMEDAD DE ALZHEIMER
NEURONAS
HIPOCAMPO
spellingShingle MEDICINA
COLESTEROL
RECEPTORES
ENFERMEDAD DE ALZHEIMER
NEURONAS
HIPOCAMPO
Borroni, María Virginia
Kamerbeek, Constanza B.
Pediconi, María F.
Barrantes, Francisco José
Lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons
topic_facet MEDICINA
COLESTEROL
RECEPTORES
ENFERMEDAD DE ALZHEIMER
NEURONAS
HIPOCAMPO
description Abstract: Neuronal 7 and 4 2 are the predominant nicotinic acetylcholine receptor (nAChR) subtypes found in the brain, particularly in the hippocampus. The e ects of lovastatin, an inhibitor of cholesterol biosynthesis, on these two nAChRs endogenously expressed in rat hippocampal neuronal cells were evaluated in the 0.01–1 M range. Chronic (14 days) lovastatin treatment augmented cell-surface levels of 7 and 4 nAChRs, as measured by fluorescence microscopy and radioactive ligand binding assays. This was accompanied in both cases by an increase in total protein receptor levels as determined byWestern blots. At low lovastatin concentrations (10–100 nM), the increase in 4 nAChR in neurites was higher than in neuronal cell somata; the opposite occurred at higher (0.5–1 M) lovastatin concentrations. In contrast, neurite 7 nAChRs raised more than somatic 7 nAChRs at all lovastatin concentrations tested. These results indicate that cholesterol levels homeostatically regulate 7 and 4 nAChR levels in a di erential manner through mechanisms that depend on statin concentration and receptor localization. The neuroprotective pleomorphic e ects of statins may act by reestablishing the homeostatic equilibrium.
format Artículo
author Borroni, María Virginia
Kamerbeek, Constanza B.
Pediconi, María F.
Barrantes, Francisco José
author_facet Borroni, María Virginia
Kamerbeek, Constanza B.
Pediconi, María F.
Barrantes, Francisco José
author_sort Borroni, María Virginia
title Lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons
title_short Lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons
title_full Lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons
title_fullStr Lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons
title_full_unstemmed Lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons
title_sort lovastatin di erentially regulates 7 and 4 neuronal nicotinic acetylcholine receptor levels in rat hippocampal neurons
publisher MDPI
publishDate 2020
url https://repositorio.uca.edu.ar/handle/123456789/10847
work_keys_str_mv AT borronimariavirginia lovastatindierentiallyregulates7and4neuronalnicotinicacetylcholinereceptorlevelsinrathippocampalneurons
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bdutipo_str Repositorios
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