Analysis of cholesterol in mouse brain by HPLC with UV detection

Abstract: We describe a sensitive high performance liquid chromatography (HPLC)-based method for the determination of cholesterol in brain tissue. The method does not require the derivatization of the analyte and uses separation and quantification by reversed-phase HPLC coupled to UV detection. Li...

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Detalles Bibliográficos
Autores principales: Paulazo, Maria Alejandra, Sodero, Alejandro Omar
Formato: Artículo
Lenguaje:Inglés
Publicado: Public Library of Science 2020
Materias:
COLESTEROL
CEREBRO
CROMATOGRAFIA LIQUIDA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/10232
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-10232
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic COLESTEROL
CEREBRO
CROMATOGRAFIA LIQUIDA
spellingShingle COLESTEROL
CEREBRO
CROMATOGRAFIA LIQUIDA
Paulazo, Maria Alejandra
Sodero, Alejandro Omar
Analysis of cholesterol in mouse brain by HPLC with UV detection
topic_facet COLESTEROL
CEREBRO
CROMATOGRAFIA LIQUIDA
description Abstract: We describe a sensitive high performance liquid chromatography (HPLC)-based method for the determination of cholesterol in brain tissue. The method does not require the derivatization of the analyte and uses separation and quantification by reversed-phase HPLC coupled to UV detection. Lipids were methanol/chloroform extracted following the method of Bligh and Dyer, and separated using isopropanol/acetonitrile/water (60/30/10, v/v/v) as mobile phase. We observed lineal detection in a wide range of concentrations, from 62.5 to 2000 ng/μL, and were able to detect a significant increase in the brain cholesterol levels between postnatal days 2 and 10 in C57BL6 mice. Based on our validation parameters, we consider this analytical method a useful tool to assess free cholesterol in rodent brain samples and cell cultures.
format Artículo
author Paulazo, Maria Alejandra
Sodero, Alejandro Omar
author_facet Paulazo, Maria Alejandra
Sodero, Alejandro Omar
author_sort Paulazo, Maria Alejandra
title Analysis of cholesterol in mouse brain by HPLC with UV detection
title_short Analysis of cholesterol in mouse brain by HPLC with UV detection
title_full Analysis of cholesterol in mouse brain by HPLC with UV detection
title_fullStr Analysis of cholesterol in mouse brain by HPLC with UV detection
title_full_unstemmed Analysis of cholesterol in mouse brain by HPLC with UV detection
title_sort analysis of cholesterol in mouse brain by hplc with uv detection
publisher Public Library of Science
publishDate 2020
url https://repositorio.uca.edu.ar/handle/123456789/10232
work_keys_str_mv AT paulazomariaalejandra analysisofcholesterolinmousebrainbyhplcwithuvdetection
AT soderoalejandroomar analysisofcholesterolinmousebrainbyhplcwithuvdetection
bdutipo_str Repositorios
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