Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu

Abstract: Hampered by the diffraction phenomenon, as expressed in 1873 by Abbe, applications of optical microscopy to image biological structures were for a long time limited to resolutions above the ~200 nm barrier and restricted to the observation of stained specimens. The introduction of fluor...

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Detalles Bibliográficos
Autor principal: Barrantes, Francisco José
Formato: Artículo
Lenguaje:Inglés
Publicado: Frontiers Media 2024
Materias:
MEMBRANA PLASMATICA
INTERACCIONES PROTEÍNA-PROTEÍNA
COLESTEROL
NANOSCOPIA
MICROSCOPIO FLUORESCENTE
RECEPTOR DE ACETILCOLINA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/18149
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-18149
record_format dspace
spelling I33-R139-123456789-181492024-05-24T05:01:55Z Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu Barrantes, Francisco José MEMBRANA PLASMATICA INTERACCIONES PROTEÍNA-PROTEÍNA COLESTEROL NANOSCOPIA MICROSCOPIO FLUORESCENTE RECEPTOR DE ACETILCOLINA Abstract: Hampered by the diffraction phenomenon, as expressed in 1873 by Abbe, applications of optical microscopy to image biological structures were for a long time limited to resolutions above the ~200 nm barrier and restricted to the observation of stained specimens. The introduction of fluorescence was a game changer, and since its inception it became the gold standard technique in biological microscopy. The plasma membrane is a tenuous envelope of 4 nm–10 nm in thickness surrounding the cell. Because of its highly versatile spectroscopic properties and availability of suitable instrumentation, fluorescence techniques epitomize the current approach to study this delicate structure and its molecular constituents. The wide spectral range covered by fluorescence, intimately linked to the availability of appropriate intrinsic and extrinsic probes, provides the ability to dissect membrane constituents at the molecular scale in the spatial domain. In addition, the time resolution capabilities of fluorescence methods provide complementary high precision for studying the behavior of membrane molecules in the time domain. This review illustrates the value of various fluorescence techniques to extract information on the topography and motion of plasma membrane receptors. To this end I resort to a paradigmatic membrane-bound neurotransmitter receptor, the nicotinic acetylcholine receptor (nAChR). The structural and dynamic picture emerging from studies of this prototypic pentameric ligand-gated ion channel can be extrapolated not only to other members of this superfamily of ion channels but to other membrane-bound proteins. I also briefly discuss the various emerging techniques in the field of biomembrane labeling with new organic chemistry strategies oriented to applications in fluorescence nanoscopy, the form of fluorescence microscopy that is expanding the depth and scope of interrogation of membrane-associated phenomena. 2024-05-23T12:03:05Z 2024-05-23T12:03:05Z 2022 Artículo Barrantes, F. J. Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu [en línea]. Frontiers in Molecular Biosciences. 2022, 9:1014659. doi: 10.3389/fmolb.2022.1014659. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/18149 https://repositorio.uca.edu.ar/handle/123456789/18149 10.3389/fmolb.2022.1014659 eng Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Frontiers Media Frontiers in Molecular Biosciences. 2022, 9:1014659
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic MEMBRANA PLASMATICA
INTERACCIONES PROTEÍNA-PROTEÍNA
COLESTEROL
NANOSCOPIA
MICROSCOPIO FLUORESCENTE
RECEPTOR DE ACETILCOLINA
spellingShingle MEMBRANA PLASMATICA
INTERACCIONES PROTEÍNA-PROTEÍNA
COLESTEROL
NANOSCOPIA
MICROSCOPIO FLUORESCENTE
RECEPTOR DE ACETILCOLINA
Barrantes, Francisco José
Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu
topic_facet MEMBRANA PLASMATICA
INTERACCIONES PROTEÍNA-PROTEÍNA
COLESTEROL
NANOSCOPIA
MICROSCOPIO FLUORESCENTE
RECEPTOR DE ACETILCOLINA
description Abstract: Hampered by the diffraction phenomenon, as expressed in 1873 by Abbe, applications of optical microscopy to image biological structures were for a long time limited to resolutions above the ~200 nm barrier and restricted to the observation of stained specimens. The introduction of fluorescence was a game changer, and since its inception it became the gold standard technique in biological microscopy. The plasma membrane is a tenuous envelope of 4 nm–10 nm in thickness surrounding the cell. Because of its highly versatile spectroscopic properties and availability of suitable instrumentation, fluorescence techniques epitomize the current approach to study this delicate structure and its molecular constituents. The wide spectral range covered by fluorescence, intimately linked to the availability of appropriate intrinsic and extrinsic probes, provides the ability to dissect membrane constituents at the molecular scale in the spatial domain. In addition, the time resolution capabilities of fluorescence methods provide complementary high precision for studying the behavior of membrane molecules in the time domain. This review illustrates the value of various fluorescence techniques to extract information on the topography and motion of plasma membrane receptors. To this end I resort to a paradigmatic membrane-bound neurotransmitter receptor, the nicotinic acetylcholine receptor (nAChR). The structural and dynamic picture emerging from studies of this prototypic pentameric ligand-gated ion channel can be extrapolated not only to other members of this superfamily of ion channels but to other membrane-bound proteins. I also briefly discuss the various emerging techniques in the field of biomembrane labeling with new organic chemistry strategies oriented to applications in fluorescence nanoscopy, the form of fluorescence microscopy that is expanding the depth and scope of interrogation of membrane-associated phenomena.
format Artículo
author Barrantes, Francisco José
author_facet Barrantes, Francisco José
author_sort Barrantes, Francisco José
title Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu
title_short Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu
title_full Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu
title_fullStr Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu
title_full_unstemmed Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu
title_sort fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu
publisher Frontiers Media
publishDate 2024
url https://repositorio.uca.edu.ar/handle/123456789/18149
work_keys_str_mv AT barrantesfranciscojose fluorescencemicroscopyimagingofaneurotransmitterreceptoranditscellmembranelipidmilieu
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