High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors

Abstract: Industrial anaerobic digestion (AD) produces biogas and a digestate that is usually applied as a biofertilizer. However, the study and application of this by-product in terms of its rich microbial diversity and high metabolic activity have been barely investigated. In this work, the digest...

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Autores principales: Olivera, Camila, Tondo, María Laura, Girardi, Valentina, Herrero, María Sol, Balaban, Cecilia Lucía, Salvatierra, Lucas Matías
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2024
Materias:
BIODEGRADACION
BIOGAS
CONSORCIO
DIESEL
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/18077
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-18077
record_format dspace
spelling I33-R139-123456789-180772024-05-10T05:01:49Z High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors Olivera, Camila Tondo, María Laura Girardi, Valentina Herrero, María Sol Balaban, Cecilia Lucía Salvatierra, Lucas Matías BIODEGRADACION BIOGAS CONSORCIO DIESEL Abstract: Industrial anaerobic digestion (AD) produces biogas and a digestate that is usually applied as a biofertilizer. However, the study and application of this by-product in terms of its rich microbial diversity and high metabolic activity have been barely investigated. In this work, the digestate regarded as an inoculum-without any further manipulation-was faced to a target hydrocarbon (i.e., diesel oil) to explore its biodegradation capability and potential application in bioaugmentation strategies. Lab-scale single batch bioreactors with solid support (i.e., sand or gravel) embedded with the inoculum and diesel were used to improve bioaccessibility and biofilm formation. In addition, different experimental conditions were assayed varying the initial diesel concentration, microbial load, type of solid support, inoculum aging time, and presence or absence of oxygen. Remaining diesel concentration, dehydrogenase activity and microbial community structure were periodically determined. Remarkably, this low-cost consortium was capable of a significant reduction (>90%) in the concentration of diesel, within 14 days and when the initial load was as high as 6950 mg/kg dry solid support. Furthermore, a 10-fold increment in dehydrogenase activity, alongside an increase in the abundance of hydrocarbon-degrading bacterial groups, and the enrichment of genes for alkane monooxygenase and aromatic ring-hydroxylating dioxygenases, encourage further study of this consortium for bioremediation purposes. 2024-05-09T18:54:59Z 2024-05-09T18:54:59Z 2024 Artículo Olivera, C., Tondo, M. L., Girardi, V., Herrero, M. S., Balaban, C. L., Salvatierra, L. M. High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors [en línea]. Postprint del artículo publicado en: Chemosphere. 2024 Mar;352:141384. doi: 10.1016/j.chemosphere.2024.141384. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/18077 https://repositorio.uca.edu.ar/handle/123456789/18077 10.1016/j.chemosphere.2024.141384 38350516 eng Acceso abierto. Embargo 24 meses http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Elsevier Postprint del artículo publicado en: Chemosphere. 2024 Mar;352:141384
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic BIODEGRADACION
BIOGAS
CONSORCIO
DIESEL
spellingShingle BIODEGRADACION
BIOGAS
CONSORCIO
DIESEL
Olivera, Camila
Tondo, María Laura
Girardi, Valentina
Herrero, María Sol
Balaban, Cecilia Lucía
Salvatierra, Lucas Matías
High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors
topic_facet BIODEGRADACION
BIOGAS
CONSORCIO
DIESEL
description Abstract: Industrial anaerobic digestion (AD) produces biogas and a digestate that is usually applied as a biofertilizer. However, the study and application of this by-product in terms of its rich microbial diversity and high metabolic activity have been barely investigated. In this work, the digestate regarded as an inoculum-without any further manipulation-was faced to a target hydrocarbon (i.e., diesel oil) to explore its biodegradation capability and potential application in bioaugmentation strategies. Lab-scale single batch bioreactors with solid support (i.e., sand or gravel) embedded with the inoculum and diesel were used to improve bioaccessibility and biofilm formation. In addition, different experimental conditions were assayed varying the initial diesel concentration, microbial load, type of solid support, inoculum aging time, and presence or absence of oxygen. Remaining diesel concentration, dehydrogenase activity and microbial community structure were periodically determined. Remarkably, this low-cost consortium was capable of a significant reduction (>90%) in the concentration of diesel, within 14 days and when the initial load was as high as 6950 mg/kg dry solid support. Furthermore, a 10-fold increment in dehydrogenase activity, alongside an increase in the abundance of hydrocarbon-degrading bacterial groups, and the enrichment of genes for alkane monooxygenase and aromatic ring-hydroxylating dioxygenases, encourage further study of this consortium for bioremediation purposes.
format Artículo
author Olivera, Camila
Tondo, María Laura
Girardi, Valentina
Herrero, María Sol
Balaban, Cecilia Lucía
Salvatierra, Lucas Matías
author_facet Olivera, Camila
Tondo, María Laura
Girardi, Valentina
Herrero, María Sol
Balaban, Cecilia Lucía
Salvatierra, Lucas Matías
author_sort Olivera, Camila
title High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors
title_short High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors
title_full High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors
title_fullStr High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors
title_full_unstemmed High-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors
title_sort high-performance diesel biodegradation using biogas digestate as microbial inoculum in lab-scale solid supported bioreactors
publisher Elsevier
publishDate 2024
url https://repositorio.uca.edu.ar/handle/123456789/18077
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