Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice

Abstract: Introduction: The circadian system synchronizes behavior and physiology to the 24-h light– dark (LD) cycle. Timing of food intake and fasting periods provide strong signals for peripheral circadian clocks regulating nutrient assimilation, glucose, and lipid metabolism. Mice under 12h li...

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Autores principales: Trebucq, Laura Lucía, Lamberti, Melisa Luciana, Rota, Rosana, Borio, Cristina, Bilen, Marcos, Golombek, Diego A., Plano, Santiago Andrés, Chiesa, Juan José
Formato: Artículo
Lenguaje:Inglés
Publicado: Frontiers Media 2023
Materias:
RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/16477
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-16477
record_format dspace
spelling I33-R139-123456789-164772024-03-21T18:48:58Z Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice Trebucq, Laura Lucía Lamberti, Melisa Luciana Rota, Rosana Borio, Cristina Bilen, Marcos Golombek, Diego A. Plano, Santiago Andrés Chiesa, Juan José RITMO CIRCADIANO INTOLERANCIA GLUCOSA LIPIDOS NUTRIENTES AUMENTO DE PESO METABOLISMO Abstract: Introduction: The circadian system synchronizes behavior and physiology to the 24-h light– dark (LD) cycle. Timing of food intake and fasting periods provide strong signals for peripheral circadian clocks regulating nutrient assimilation, glucose, and lipid metabolism. Mice under 12h light:12h dark (LD) cycles exhibit behavioral activity and feeding during the dark period, while fasting occurs at rest during light. Disruption of energy metabolism, leading to an increase in body mass, was reported in experimental models of circadian desynchronization. In this work, the effects of chronic advances of the LD cycles (chronic jet-lag protocol, CJL) were studied on the daily homeostasis of energy metabolism and weight gain. Methods: Male C57 mice were subjected to a CJL or LD schedule, measuring IPGTT, insulinemia, microbiome composition and lipidemia. Results: Mice under CJL show behavioral desynchronization and feeding activity distributed similarly at the light and dark hours and, although feeding a similar daily amount of food as compared to controls, show an increase in weight gain. In addition, ad libitum glycemia rhythm was abolished in CJL-subjected mice, showing similar blood glucose values at light and dark. CJL also generated glucose intolerance at dark in an intraperitoneal glucose tolerance test (IPGTT), with increased insulin release at both light and dark periods. Low-density lipoprotein (LDL) cholesterolemia was increased under this condition, but no changes in HDL cholesterolemia were observed. Firmicutes/ Bacteroidetes ratio was analyzed as a marker of circadian disruption of microbiota composition, showing opposite phases at the light and dark when comparing LD vs. CJL. Discussion: Chronic misalignment of feeding/fasting rhythm leads to metabolic disturbances generating nocturnal hyperglycemia, glucose intolerance and hyperinsulinemia in a IPGTT, increased LDL cholesterolemia, and increased weight gain, underscoring the importance of the timing of food consumption with respect to the circadian system for metabolic health. 2023-06-06T13:40:54Z 2023-06-06T13:40:54Z 2023 Artículo Trebucq, L. L. et al. Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice [en línea]. Frontiers in Nutrition. 2023, 10. doi:10.3389/fnut.2023.1154647. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16477 2296-861X (online) https://repositorio.uca.edu.ar/handle/123456789/16477 10.3389/fnut.2023.1154647 37125029 eng Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Frontiers Media Frontiers in Nutrition. 2023, 10
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
spellingShingle RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
Trebucq, Laura Lucía
Lamberti, Melisa Luciana
Rota, Rosana
Borio, Cristina
Bilen, Marcos
Golombek, Diego A.
Plano, Santiago Andrés
Chiesa, Juan José
Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
topic_facet RITMO CIRCADIANO
INTOLERANCIA
GLUCOSA
LIPIDOS
NUTRIENTES
AUMENTO DE PESO
METABOLISMO
description Abstract: Introduction: The circadian system synchronizes behavior and physiology to the 24-h light– dark (LD) cycle. Timing of food intake and fasting periods provide strong signals for peripheral circadian clocks regulating nutrient assimilation, glucose, and lipid metabolism. Mice under 12h light:12h dark (LD) cycles exhibit behavioral activity and feeding during the dark period, while fasting occurs at rest during light. Disruption of energy metabolism, leading to an increase in body mass, was reported in experimental models of circadian desynchronization. In this work, the effects of chronic advances of the LD cycles (chronic jet-lag protocol, CJL) were studied on the daily homeostasis of energy metabolism and weight gain. Methods: Male C57 mice were subjected to a CJL or LD schedule, measuring IPGTT, insulinemia, microbiome composition and lipidemia. Results: Mice under CJL show behavioral desynchronization and feeding activity distributed similarly at the light and dark hours and, although feeding a similar daily amount of food as compared to controls, show an increase in weight gain. In addition, ad libitum glycemia rhythm was abolished in CJL-subjected mice, showing similar blood glucose values at light and dark. CJL also generated glucose intolerance at dark in an intraperitoneal glucose tolerance test (IPGTT), with increased insulin release at both light and dark periods. Low-density lipoprotein (LDL) cholesterolemia was increased under this condition, but no changes in HDL cholesterolemia were observed. Firmicutes/ Bacteroidetes ratio was analyzed as a marker of circadian disruption of microbiota composition, showing opposite phases at the light and dark when comparing LD vs. CJL. Discussion: Chronic misalignment of feeding/fasting rhythm leads to metabolic disturbances generating nocturnal hyperglycemia, glucose intolerance and hyperinsulinemia in a IPGTT, increased LDL cholesterolemia, and increased weight gain, underscoring the importance of the timing of food consumption with respect to the circadian system for metabolic health.
format Artículo
author Trebucq, Laura Lucía
Lamberti, Melisa Luciana
Rota, Rosana
Borio, Cristina
Bilen, Marcos
Golombek, Diego A.
Plano, Santiago Andrés
Chiesa, Juan José
author_facet Trebucq, Laura Lucía
Lamberti, Melisa Luciana
Rota, Rosana
Borio, Cristina
Bilen, Marcos
Golombek, Diego A.
Plano, Santiago Andrés
Chiesa, Juan José
author_sort Trebucq, Laura Lucía
title Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_short Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_full Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_fullStr Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_full_unstemmed Chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, LDL cholesterolemia and microbiota composition in mice
title_sort chronic circadian desynchronization of feeding-fasting rhythm generates alterations in daily glycemia, ldl cholesterolemia and microbiota composition in mice
publisher Frontiers Media
publishDate 2023
url https://repositorio.uca.edu.ar/handle/123456789/16477
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