Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer
Abstract: Triple negative breast cancer (TNBC) is the most aggressive breast cancer subtype. Currently, paclitaxel (PTX) represents the first-line therapy for TNBC; however it presents a hydrophobic behavior and produces severe adverse effects. The aim of this work is to improve the therapeutic i...
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| Lenguaje: | Inglés |
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2023
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| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/16409 |
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I33-R139-123456789-164092023-11-23T17:45:14Z Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer Nicoud, Melisa Beatriz Ospital, Ignacio Táquez Delgado, Mónica Alejandra Riedel, Jennifer Fuentes, Pedro Bernabeu, Ezequiel Rubinstein Guichón, Mara Roxana Lauretta, Paolo Martínez Vivot, Rocío Aguilar, María de los Angeles Salgueiro, María J. Speisky, Daniela Moretton, Marcela A. Chiappetta, Diego A. Medina, Vanina A. 0000-0003-2513-5975 CANCER DE MAMA TRIPLE NEGATIVO HISTAMINA FORMULACION NANOMICELAR TERAPIA DE COMBINACION MICELAS POLIMERICAS APOPTOSIS Abstract: Triple negative breast cancer (TNBC) is the most aggressive breast cancer subtype. Currently, paclitaxel (PTX) represents the first-line therapy for TNBC; however it presents a hydrophobic behavior and produces severe adverse effects. The aim of this work is to improve the therapeutic index of PTX through the design and characterization of novel nanomicellar polymeric formulations composed of a biocompatible copolymer Soluplus® (S), surface-decorated with glucose (GS), and co-loaded either with histamine (HA, 5 mg/mL) and/or PTX (4 mg/mL). Their micellar size, evaluated by dynamic light scattering, showed a hydrodynamic diameter between 70 and 90 nm for loaded nanoformulations with a unimodal size distribution. Cytotoxicity and apoptosis assays were performed to assess their efficacy in vitro in human MDA-MB-231 and murine 4T1 TNBC cells rendering optimal antitumor efficacy in both cell lines for the nanoformulations with both drugs. In a model of TNBC developed in BALB/c mice with 4T1 cells, we found that all loaded micellar systems reduced tumor volume and that both HA and HA-PTX-loaded SG micelles reduced tumor weight and neovascularization compared with the empty micelles. We conclude that HA-PTX co-loaded micelles in addition to HA-loaded formulations present promising potential as nano-drug delivery systems for cancer chemotherapy. 2023-05-29T10:44:51Z 2023-05-29T10:44:51Z 2023 Artículo Nicoud, M.B., et al. Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer [en línea]. International Journal of Molecular Sciences. 2023, 24(4) doi: 10.3390/ijms24043546. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16409 1422-0067 https://repositorio.uca.edu.ar/handle/123456789/16409 10.3390/ijms24043546 36834958 eng Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf MDPI International Journal of Molecular Sciences. 2023, 24(4) |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
CANCER DE MAMA TRIPLE NEGATIVO HISTAMINA FORMULACION NANOMICELAR TERAPIA DE COMBINACION MICELAS POLIMERICAS APOPTOSIS |
| spellingShingle |
CANCER DE MAMA TRIPLE NEGATIVO HISTAMINA FORMULACION NANOMICELAR TERAPIA DE COMBINACION MICELAS POLIMERICAS APOPTOSIS Nicoud, Melisa Beatriz Ospital, Ignacio Táquez Delgado, Mónica Alejandra Riedel, Jennifer Fuentes, Pedro Bernabeu, Ezequiel Rubinstein Guichón, Mara Roxana Lauretta, Paolo Martínez Vivot, Rocío Aguilar, María de los Angeles Salgueiro, María J. Speisky, Daniela Moretton, Marcela A. Chiappetta, Diego A. Medina, Vanina A. Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer |
| topic_facet |
CANCER DE MAMA TRIPLE NEGATIVO HISTAMINA FORMULACION NANOMICELAR TERAPIA DE COMBINACION MICELAS POLIMERICAS APOPTOSIS |
| description |
Abstract: Triple negative breast cancer (TNBC) is the most aggressive breast cancer subtype. Currently,
paclitaxel (PTX) represents the first-line therapy for TNBC; however it presents a hydrophobic
behavior and produces severe adverse effects. The aim of this work is to improve the therapeutic
index of PTX through the design and characterization of novel nanomicellar polymeric formulations
composed of a biocompatible copolymer Soluplus® (S), surface-decorated with glucose (GS),
and co-loaded either with histamine (HA, 5 mg/mL) and/or PTX (4 mg/mL). Their micellar size,
evaluated by dynamic light scattering, showed a hydrodynamic diameter between 70 and 90 nm
for loaded nanoformulations with a unimodal size distribution. Cytotoxicity and apoptosis assays
were performed to assess their efficacy in vitro in human MDA-MB-231 and murine 4T1 TNBC cells
rendering optimal antitumor efficacy in both cell lines for the nanoformulations with both drugs. In a
model of TNBC developed in BALB/c mice with 4T1 cells, we found that all loaded micellar systems
reduced tumor volume and that both HA and HA-PTX-loaded SG micelles reduced tumor weight
and neovascularization compared with the empty micelles. We conclude that HA-PTX co-loaded
micelles in addition to HA-loaded formulations present promising potential as nano-drug delivery
systems for cancer chemotherapy. |
| author2 |
0000-0003-2513-5975 |
| author_facet |
0000-0003-2513-5975 Nicoud, Melisa Beatriz Ospital, Ignacio Táquez Delgado, Mónica Alejandra Riedel, Jennifer Fuentes, Pedro Bernabeu, Ezequiel Rubinstein Guichón, Mara Roxana Lauretta, Paolo Martínez Vivot, Rocío Aguilar, María de los Angeles Salgueiro, María J. Speisky, Daniela Moretton, Marcela A. Chiappetta, Diego A. Medina, Vanina A. |
| format |
Artículo |
| author |
Nicoud, Melisa Beatriz Ospital, Ignacio Táquez Delgado, Mónica Alejandra Riedel, Jennifer Fuentes, Pedro Bernabeu, Ezequiel Rubinstein Guichón, Mara Roxana Lauretta, Paolo Martínez Vivot, Rocío Aguilar, María de los Angeles Salgueiro, María J. Speisky, Daniela Moretton, Marcela A. Chiappetta, Diego A. Medina, Vanina A. |
| author_sort |
Nicoud, Melisa Beatriz |
| title |
Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer |
| title_short |
Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer |
| title_full |
Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer |
| title_fullStr |
Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer |
| title_full_unstemmed |
Nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer |
| title_sort |
nanomicellar formulations loaded with histamine and paclitaxel as a new strategy to improve chemotherapy for breast cáncer |
| publisher |
MDPI |
| publishDate |
2023 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/16409 |
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1807949175730470912 |