Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats

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Abstract: Background: Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expres...

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Autores principales: Jiménez Ortega, Vanesa, Ríos Lugo, María J., Cano Barquilla, Pilar, Cardinali, Daniel Pedro, Esquifino, Ana I., Fernández Mateos, María P., Spinedi, Eduardo J.
Formato: Artículo
Lenguaje:Inglés
Inglés
Publicado: De Gruyter 2019
Materias:
MELATONINA
GRASAS EN LA DIETA
ALIMENTACION
EXPRESION GENICA
NEUROPEPTIDO Y
PROOPIOMELANOCORTINA
PROLACTINA
LEPTINA
ADIPONECTINA
HIPOTALAMO MEDIO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/1638
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-1638
record_format dspace
spelling I33-R139-123456789-16382024-03-12T11:30:13Z Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats Jiménez Ortega, Vanesa Ríos Lugo, María J. Cano Barquilla, Pilar Cardinali, Daniel Pedro Esquifino, Ana I. Fernández Mateos, María P. Spinedi, Eduardo J. MELATONINA GRASAS EN LA DIETA ALIMENTACION EXPRESION GENICA NEUROPEPTIDO Y PROOPIOMELANOCORTINA PROLACTINA LEPTINA ADIPONECTINA HIPOTALAMO MEDIO Abstract: Background: Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulinreceptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured. Methods: Adult Wistar male rats were divided into four groups (n= 16/group): (i) control diet (3 % fat); (ii) high-fat (35 %) diet; (iii) high-fat diet + melatonin; (iv) control diet + melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin. Results: After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated body weight increase in high-fat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats. Conclusions: The results indicate that an effect on gene expression of feeding behavior signals at the CNS may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats 2019-05-02T14:01:13Z 2019-05-02T14:01:13Z 2015 Artículo Ríos Lugo, M. J., et al. Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats [en línea]. Preprint del artículo publicado en Hormone Molecular Biology and Clinical Investigation. 2015, 21 (3). doi:10.1515/hmbci-2014-0041. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1638 1868-1883 (impreso) 1868-1891 (online) https://repositorio.uca.edu.ar/handle/123456789/1638 10.1515/hmbci-2014-0041 eng eng Acceso Abierto https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf De Gruyter Preprint del artículo publicado en Hormone Molecular Biology and Clinical Investigation, Vol. 21, N° 3, 2015
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
Inglés
topic MELATONINA
GRASAS EN LA DIETA
ALIMENTACION
EXPRESION GENICA
NEUROPEPTIDO Y
PROOPIOMELANOCORTINA
PROLACTINA
LEPTINA
ADIPONECTINA
HIPOTALAMO MEDIO
spellingShingle MELATONINA
GRASAS EN LA DIETA
ALIMENTACION
EXPRESION GENICA
NEUROPEPTIDO Y
PROOPIOMELANOCORTINA
PROLACTINA
LEPTINA
ADIPONECTINA
HIPOTALAMO MEDIO
Jiménez Ortega, Vanesa
Ríos Lugo, María J.
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
Fernández Mateos, María P.
Spinedi, Eduardo J.
Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
topic_facet MELATONINA
GRASAS EN LA DIETA
ALIMENTACION
EXPRESION GENICA
NEUROPEPTIDO Y
PROOPIOMELANOCORTINA
PROLACTINA
LEPTINA
ADIPONECTINA
HIPOTALAMO MEDIO
description Abstract: Background: Previous studies indicate that the administration of melatonin caused body weight and abdominal visceral fat reductions in rodent models of hyperadiposity. The objective of the present study performed in high-fat fed rats was to evaluate the activity of melatonin on gene expression of some medial basal hypothalamus (MBH) signals involved in feeding behavior regulation, including neuropeptide Y (NPY), proopiomelanocortin (POMC), prolactin-releasing peptide (PrRP), leptin- and insulinreceptors (R) and insulin-R substrate (IRS)-1 and -2. Blood levels of leptin and adiponectin were also measured. Methods: Adult Wistar male rats were divided into four groups (n= 16/group): (i) control diet (3 % fat); (ii) high-fat (35 %) diet; (iii) high-fat diet + melatonin; (iv) control diet + melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions: (a) tap water; (b) 25 μg/mL of melatonin. Results: After 10 weeks, the high-fat fed rats showed augmented MBH mRNA levels of NPY, leptin-R, PrRP, insulin-R, IRS-1 and IRS-2. The concomitant administration of melatonin counteracted this increase. Feeding of rats with a high-fat diet augmented expression of MBH POMC gene through an effect insensitive to melatonin treatment. The augmented levels of circulating leptin and adiponectin seen in high-fat fed rats were counteracted by melatonin as was the augmented body weight: melatonin significantly attenuated body weight increase in high-fat fed rats without affecting chow or water consumption. Melatonin augmented plasma leptin and adiponectin in control rats. Conclusions: The results indicate that an effect on gene expression of feeding behavior signals at the CNS may complement a peripheral rise of the energy expenditure produced by melatonin to decrease body weight in high-fat fed rats
format Artículo
author Jiménez Ortega, Vanesa
Ríos Lugo, María J.
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
Fernández Mateos, María P.
Spinedi, Eduardo J.
author_facet Jiménez Ortega, Vanesa
Ríos Lugo, María J.
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
Fernández Mateos, María P.
Spinedi, Eduardo J.
author_sort Jiménez Ortega, Vanesa
title Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_short Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_full Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_fullStr Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_full_unstemmed Melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
title_sort melatonin counteracts changes in hypothalamic gene expression of signals regulating feeding behavior in high-fat fed rats
publisher De Gruyter
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/1638
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AT spinedieduardoj melatonincounteractschangesinhypothalamicgeneexpressionofsignalsregulatingfeedingbehaviorinhighfatfedrats
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