Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model
Abstract: Background: Hindpaw injection of formalin in rodents is used to assess acute persistent pain. The response to formalin is biphasic. The initial response (first minutes) is thought to be linked to inflammatory, peripheral mechanisms, while the latter (around 30 min after the injection),...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Multidisciplinary Digital Publishing Institute
2023
|
| Materias: | |
| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/16062 |
| Aporte de: |
| id |
I33-R139-123456789-16062 |
|---|---|
| record_format |
dspace |
| spelling |
I33-R139-123456789-160622023-11-23T17:43:17Z Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model Gobetto, María Natalia Salinas Castellanos, Libia Catalina Contreras, Natalia Estefanía Sodero, Alejandro Omar Cambiagno, Damián Alejandro Mingolo Malnati, Georgina Oriana Montes, Mayra Micaela Uchitel, Osvaldo Daniel Weissmann, Carina CANALES ASIC1 miIR485-5p miARN DOLOR FORMALINA RATON Abstract: Background: Hindpaw injection of formalin in rodents is used to assess acute persistent pain. The response to formalin is biphasic. The initial response (first minutes) is thought to be linked to inflammatory, peripheral mechanisms, while the latter (around 30 min after the injection), is linked to central mechanisms. This model is useful to analyze the effect of drugs at one or both phases, and the involvement of ion channels in the response. Acid-sensing ion channels (ASICs) regulate synaptic activities and play important roles in pain conditions. Recently, psalmotoxin-1 (Pctx-1), a toxin that inhibits ASIC1a-constituted channels, and antisense ASIC1a-RNA, intrathecal administered in mice were shown to affect both phases of the test. Methods: The mouse formalin test was performed on C57/BL6 7- to 9-week-old mice. Behavioral tests were conducted and tissue was extracted to detect proteins (ASIC1 and pERK) and ASIC1-mRNA and mir485-5p levels. Results: The injection of formalin was accompanied by an increase in ASIC1 levels. This was detected at the contralateral anterior cingulate cortex (ACC) compared to the ipsilateral side, and both sides of the ACC of vehicle-injected animals. At the spinal cord and dorsal root ganglia, ASIC1 levels followed a gradient stronger at lumbar (L) 3 and decreased towards L5. Gender differences were detected at the ACC; with female mice showing higher ASIC1a levels at the ACC. No significant changes in ASIC1-mRNA levels were detected. Evidence suggests ASIC1 upregulation depends on regulatory microRNAs. Conclusion: This work highlights the important role of ASIC1 in pain and the potential role of pharmacological therapies aimed at this channel. 2023-03-23T11:45:51Z 2023-03-23T11:45:51Z 2022 Artículo Gobetto, M. N. Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model [en línea]. Pharmaceuticals. 2022, 15 (12). doi:10.3390/ph15121539. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16062 1424-8247 https://repositorio.uca.edu.ar/handle/123456789/16062 10.3390/ph15121539 36558990 eng Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Pharmaceuticals Vol.15, No.12, 2022 |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
CANALES ASIC1 miIR485-5p miARN DOLOR FORMALINA RATON |
| spellingShingle |
CANALES ASIC1 miIR485-5p miARN DOLOR FORMALINA RATON Gobetto, María Natalia Salinas Castellanos, Libia Catalina Contreras, Natalia Estefanía Sodero, Alejandro Omar Cambiagno, Damián Alejandro Mingolo Malnati, Georgina Oriana Montes, Mayra Micaela Uchitel, Osvaldo Daniel Weissmann, Carina Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model |
| topic_facet |
CANALES ASIC1 miIR485-5p miARN DOLOR FORMALINA RATON |
| description |
Abstract: Background: Hindpaw injection of formalin in rodents is used to assess acute persistent
pain. The response to formalin is biphasic. The initial response (first minutes) is thought to be
linked to inflammatory, peripheral mechanisms, while the latter (around 30 min after the injection),
is linked to central mechanisms. This model is useful to analyze the effect of drugs at one or both
phases, and the involvement of ion channels in the response. Acid-sensing ion channels (ASICs)
regulate synaptic activities and play important roles in pain conditions. Recently, psalmotoxin-1
(Pctx-1), a toxin that inhibits ASIC1a-constituted channels, and antisense ASIC1a-RNA, intrathecal
administered in mice were shown to affect both phases of the test. Methods: The mouse formalin
test was performed on C57/BL6 7- to 9-week-old mice. Behavioral tests were conducted and tissue
was extracted to detect proteins (ASIC1 and pERK) and ASIC1-mRNA and mir485-5p levels. Results:
The injection of formalin was accompanied by an increase in ASIC1 levels. This was detected at the
contralateral anterior cingulate cortex (ACC) compared to the ipsilateral side, and both sides of the
ACC of vehicle-injected animals. At the spinal cord and dorsal root ganglia, ASIC1 levels followed
a gradient stronger at lumbar (L) 3 and decreased towards L5. Gender differences were detected
at the ACC; with female mice showing higher ASIC1a levels at the ACC. No significant changes in
ASIC1-mRNA levels were detected. Evidence suggests ASIC1 upregulation depends on regulatory
microRNAs. Conclusion: This work highlights the important role of ASIC1 in pain and the potential
role of pharmacological therapies aimed at this channel. |
| format |
Artículo |
| author |
Gobetto, María Natalia Salinas Castellanos, Libia Catalina Contreras, Natalia Estefanía Sodero, Alejandro Omar Cambiagno, Damián Alejandro Mingolo Malnati, Georgina Oriana Montes, Mayra Micaela Uchitel, Osvaldo Daniel Weissmann, Carina |
| author_facet |
Gobetto, María Natalia Salinas Castellanos, Libia Catalina Contreras, Natalia Estefanía Sodero, Alejandro Omar Cambiagno, Damián Alejandro Mingolo Malnati, Georgina Oriana Montes, Mayra Micaela Uchitel, Osvaldo Daniel Weissmann, Carina |
| author_sort |
Gobetto, María Natalia |
| title |
Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model |
| title_short |
Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model |
| title_full |
Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model |
| title_fullStr |
Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model |
| title_full_unstemmed |
Segmental upregulation of ASIC1 channels in the formalin acute pain mouse model |
| title_sort |
segmental upregulation of asic1 channels in the formalin acute pain mouse model |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2023 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/16062 |
| work_keys_str_mv |
AT gobettomarianatalia segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT salinascastellanoslibiacatalina segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT contrerasnataliaestefania segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT soderoalejandroomar segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT cambiagnodamianalejandro segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT mingolomalnatigeorginaoriana segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT montesmayramicaela segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT uchitelosvaldodaniel segmentalupregulationofasic1channelsintheformalinacutepainmousemodel AT weissmanncarina segmentalupregulationofasic1channelsintheformalinacutepainmousemodel |
| _version_ |
1807949169042653184 |