Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology

Abstract: The haloarchaeal diversity of four hypersaline alkaline lakes from the Wadi El-Natrun depression (Northern Egypt) was investigated using culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes, which was combined with rem...

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Autores principales: Elshafey, Naglaa, Selim, Samy, Mohammed, Asmaa H., Hagagy, Nashwa, Samy, Mennatalla, Mostafa, Ehab M., Safhi, Fatmah A., Saddiq, Amna, Alsharari, Salam S., Aseel, Dalia G., Hafiz, Iram, Elkelish, Amr, Pérez, Leonardo Martín
Formato: Artículo
Lenguaje:Inglés
Publicado: MDPI 2022
Materias:
HALOARQUEAS
BIOTECNOLOGIA
BIODIVERSIDAD
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14809
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14809
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic HALOARQUEAS
BIOTECNOLOGIA
BIODIVERSIDAD
spellingShingle HALOARQUEAS
BIOTECNOLOGIA
BIODIVERSIDAD
Elshafey, Naglaa
Selim, Samy
Mohammed, Asmaa H.
Hagagy, Nashwa
Samy, Mennatalla
Mostafa, Ehab M.
Safhi, Fatmah A.
Saddiq, Amna
Alsharari, Salam S.
Aseel, Dalia G.
Hafiz, Iram
Elkelish, Amr
Pérez, Leonardo Martín
Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology
topic_facet HALOARQUEAS
BIOTECNOLOGIA
BIODIVERSIDAD
description Abstract: The haloarchaeal diversity of four hypersaline alkaline lakes from the Wadi El-Natrun depression (Northern Egypt) was investigated using culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes, which was combined with remote sensing and geographic information system (GIS) data to highlight the distribution pattern of the microbial diversity in water and sediment samples. The majority of archaeal sequences identified in all four lakes belonged to the phyla Euryarchaeota and Crenarchaeota. Sediment samples from Beida Lake and water samples from El-Hamra Lake showed the highest levels of archaeal diversity. Sequence similarities ≥ 95% were found between six of the acquired clones and uncultured Halorhabdus, Euryarchaeota, and archaeon clones. In addition, two clones shared a high level of sequence similarity (97%) with unclassified archaea, while other nine clones exhibited 96% to 99% sequence similarity with uncultured archaeon clones, and only one clone showed 97% identity with an uncultured Crenarchaeota. Likewise, 7 DGGE bands presented a sequence similarity of 90 to 98% to Halogranum sp., Halalkalicoccus tibetensis, Halalkalicoccus jeotgali, uncultured Halorubrum, Halobacteriaceae sp., or uncultured haloarchaeon. In conclusion, while the variety of alkaliphilic haloarchaea in the examined soda lakes was restricted, the possibility of uncovering novel species for biotechnological applications from these extreme habitats remains promising.
format Artículo
author Elshafey, Naglaa
Selim, Samy
Mohammed, Asmaa H.
Hagagy, Nashwa
Samy, Mennatalla
Mostafa, Ehab M.
Safhi, Fatmah A.
Saddiq, Amna
Alsharari, Salam S.
Aseel, Dalia G.
Hafiz, Iram
Elkelish, Amr
Pérez, Leonardo Martín
author_facet Elshafey, Naglaa
Selim, Samy
Mohammed, Asmaa H.
Hagagy, Nashwa
Samy, Mennatalla
Mostafa, Ehab M.
Safhi, Fatmah A.
Saddiq, Amna
Alsharari, Salam S.
Aseel, Dalia G.
Hafiz, Iram
Elkelish, Amr
Pérez, Leonardo Martín
author_sort Elshafey, Naglaa
title Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology
title_short Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology
title_full Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology
title_fullStr Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology
title_full_unstemmed Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology
title_sort mapping archaeal diversity in soda lakes by coupling 16s rrna pcr-dgge analysis with remote sensing and gis technology
publisher MDPI
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14809
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