Fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes

Abstract: In attempting to interrogate the structural and functional properties of cell membranes with minimal invasiveness, fluorescence microscopy remains the gold-standard technique. Fluorescence is a highly versatile spectroscopic property covering a wide range of the spectrum, with inherent adv...

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Autor principal: Barrantes, Francisco José
Formato: Parte de libro
Lenguaje:Inglés
Publicado: Academic Press 2022
Materias:
FLUORESCENCIA
COLESTEROL
MEMBRANAS CELULARES
LIPIDOS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14434
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14434
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic FLUORESCENCIA
COLESTEROL
MEMBRANAS CELULARES
LIPIDOS
spellingShingle FLUORESCENCIA
COLESTEROL
MEMBRANAS CELULARES
LIPIDOS
Barrantes, Francisco José
Fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes
topic_facet FLUORESCENCIA
COLESTEROL
MEMBRANAS CELULARES
LIPIDOS
description Abstract: In attempting to interrogate the structural and functional properties of cell membranes with minimal invasiveness, fluorescence microscopy remains the gold-standard technique. Fluorescence is a highly versatile spectroscopic property covering a wide range of the spectrum, with inherent advantages in both the spatial and time domains. Fluorescent probes have been key elements in the constellation of tools used to push the limits of spatial resolution down to single-molecule localization, optimize visualization of delicate biological structures in the living cell, and enable the tracking of molecular motions with high temporal precision. Cholesterol is an essential component of eukaryotic membranes; it interacts with other lipids and proteins and has a modulatory function on many membrane-resident hormone and neurotransmitter receptors. Fluorescent sensors have played an important role in revealing different biological and physicochemical properties of cholesterol. In cell biology applications, fluorescent cholesterol sensors helped determine uptake mechanisms and topography in the plasma membrane and follow the intracellular trafficking of cholesterol. Biophysical investigations have resorted to fluorescent probes to establish orientation, rotational, and translational motions in the membrane, order/disorder, and physical changes brought about by the presence of the sterol in the phospholipid bilayer. The ample spectral range and varying specificities of direct and indirect probes designed to visualize cholesterol enable the experimentalist to study a wide array of cholesterol properties and/or learn about the influence of the sterol on other lipids, membrane proteins, and transient supramolecular assemblies like membrane-resident lipid domains.
format Parte de libro
author Barrantes, Francisco José
author_facet Barrantes, Francisco José
author_sort Barrantes, Francisco José
title Fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes
title_short Fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes
title_full Fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes
title_fullStr Fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes
title_full_unstemmed Fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes
title_sort fluorescent probes for microscopy visualization of cholesterol topography and dynamics in membranes
publisher Academic Press
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14434
work_keys_str_mv AT barrantesfranciscojose fluorescentprobesformicroscopyvisualizationofcholesteroltopographyanddynamicsinmembranes
bdutipo_str Repositorios
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