Angiotensin-II-evoked Ca2+ entry in murine cardiac gibroblasts does not depend on TRPC channels

Abstract: TRPC proteins form cation conducting channels regulated by different stimuli and are regulators of the cellular calcium homeostasis. TRPC are expressed in cardiac cells including cardiac fibroblasts (CFs) and have been implicated in the development of pathological cardiac remodeling includ...

Descripción completa

Detalles Bibliográficos
Autores principales: Camacho Londoño, Juan E., Marx, André, Kraft, Axel E., Schürger, Alexander, Richter, Christin, Dietrich, Alexander, Lipp, Peter, Birnbaumer, Lutz, Freichel, Marc
Formato: Artículo
Lenguaje:Español
Publicado: MDPI 2022
Materias:
CALCIO
MIOCARDIO
GENES
TRPC
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14246
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14246
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Español
topic CALCIO
MIOCARDIO
GENES
TRPC
spellingShingle CALCIO
MIOCARDIO
GENES
TRPC
Camacho Londoño, Juan E.
Marx, André
Kraft, Axel E.
Schürger, Alexander
Richter, Christin
Dietrich, Alexander
Lipp, Peter
Birnbaumer, Lutz
Freichel, Marc
Angiotensin-II-evoked Ca2+ entry in murine cardiac gibroblasts does not depend on TRPC channels
topic_facet CALCIO
MIOCARDIO
GENES
TRPC
description Abstract: TRPC proteins form cation conducting channels regulated by different stimuli and are regulators of the cellular calcium homeostasis. TRPC are expressed in cardiac cells including cardiac fibroblasts (CFs) and have been implicated in the development of pathological cardiac remodeling including fibrosis. Using Ca2+ imaging and several compound TRPC knockout mouse lines we analyzed the involvement of TRPC proteins for the angiotensin II (AngII)-induced changes in Ca2+ homeostasis in CFs isolated from adult mice. Using qPCR we detected transcripts of all Trpc genes in CFs; Trpc1, Trpc3 and Trpc4 being the most abundant ones. We show that the AngII-induced Ca2+ entry but also Ca2+ release from intracellular stores are critically dependent on the density of CFs in culture and are inversely correlated with the expression of the myofibroblast marker α-smooth muscle actin. Our Ca2+ measurements depict that the AngII- and thrombin-induced Ca2+ transients, and the AngII-induced Ca2+ entry and Ca2+ release are not affected in CFs isolated from mice lacking all seven TRPC proteins (TRPC-hepta KO) compared to control cells. However, pre-incubation with GSK7975A (10 µM), which sufficiently inhibits CRAC channels in other cells, abolished AngII-induced Ca2+ entry. Consequently, we conclude the dispensability of the TRPC channels for the acute neurohumoral Ca2+ signaling evoked by AngII in isolated CFs and suggest the contribution of members of the Orai channel family as molecular constituents responsible for this pathophysiologically important Ca2+ entry pathway.
format Artículo
author Camacho Londoño, Juan E.
Marx, André
Kraft, Axel E.
Schürger, Alexander
Richter, Christin
Dietrich, Alexander
Lipp, Peter
Birnbaumer, Lutz
Freichel, Marc
author_facet Camacho Londoño, Juan E.
Marx, André
Kraft, Axel E.
Schürger, Alexander
Richter, Christin
Dietrich, Alexander
Lipp, Peter
Birnbaumer, Lutz
Freichel, Marc
author_sort Camacho Londoño, Juan E.
title Angiotensin-II-evoked Ca2+ entry in murine cardiac gibroblasts does not depend on TRPC channels
title_short Angiotensin-II-evoked Ca2+ entry in murine cardiac gibroblasts does not depend on TRPC channels
title_full Angiotensin-II-evoked Ca2+ entry in murine cardiac gibroblasts does not depend on TRPC channels
title_fullStr Angiotensin-II-evoked Ca2+ entry in murine cardiac gibroblasts does not depend on TRPC channels
title_full_unstemmed Angiotensin-II-evoked Ca2+ entry in murine cardiac gibroblasts does not depend on TRPC channels
title_sort angiotensin-ii-evoked ca2+ entry in murine cardiac gibroblasts does not depend on trpc channels
publisher MDPI
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14246
work_keys_str_mv AT camacholondonojuane angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT marxandre angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT kraftaxele angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT schurgeralexander angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT richterchristin angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT dietrichalexander angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT lipppeter angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT birnbaumerlutz angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
AT freichelmarc angiotensiniievokedca2entryinmurinecardiacgibroblastsdoesnotdependontrpcchannels
bdutipo_str Repositorios
_version_ 1764820524027871233

Ejemplares similares