Antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4T1 murine breast cancer model. Comparative studies using Doxil® and free doxorubicin

Abstract: Doxorubicin-loaded mixed micelles (DOX-micelles) were formulated employing TPGS and a poloxamine (Tetronic® T1107) in order to investigate their pharmaceutical application as an alternative anticancer nanotherapy. DOX-micelles presented unimodal behaviour, exhibiting average size values of...

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Detalles Bibliográficos
Autores principales: Cagel, Maximiliano, Moretton, Marcela A., Bernabeu, Ezequiel, Zubillaga, Marcelo, Lagomarsino, Eduardo, Vanzulli, Silvia, Nicoud, Melisa Beatriz, Medina, Vanina Araceli, Salgueiro, María J., Chiappetta, Diego A.
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
CANCER DE MAMA
TRATAMIENTO FARMACOLOGICO
MICELAS
DOXORRUBICINA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14229
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14229
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic CANCER DE MAMA
TRATAMIENTO FARMACOLOGICO
MICELAS
DOXORRUBICINA
spellingShingle CANCER DE MAMA
TRATAMIENTO FARMACOLOGICO
MICELAS
DOXORRUBICINA
Cagel, Maximiliano
Moretton, Marcela A.
Bernabeu, Ezequiel
Zubillaga, Marcelo
Lagomarsino, Eduardo
Vanzulli, Silvia
Nicoud, Melisa Beatriz
Medina, Vanina Araceli
Salgueiro, María J.
Chiappetta, Diego A.
Antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4T1 murine breast cancer model. Comparative studies using Doxil® and free doxorubicin
topic_facet CANCER DE MAMA
TRATAMIENTO FARMACOLOGICO
MICELAS
DOXORRUBICINA
description Abstract: Doxorubicin-loaded mixed micelles (DOX-micelles) were formulated employing TPGS and a poloxamine (Tetronic® T1107) in order to investigate their pharmaceutical application as an alternative anticancer nanotherapy. DOX-micelles presented unimodal behaviour, exhibiting average size values of 10.7 ± 0.2 nm and 10.4 ± 0.1 nm, before and after lyophilization, respectively. The in vitro cytotoxic effect of DOX-micelles was significantly higher (p < 0.05) than Doxil® on 4T1 murine breast cancer cells. The in vitro cell-based internalization assays showed a significant augment (p < 0.05) of the intracellular DOX content for the DOX-micelles in comparison to the liposomes and free DOX in these 4T1 cells at 2 and 6 h. Besides, DOX-micelles presented higher in vivo anticancer effect than Doxil® and equivalent potency to the free drug. Moreover, the in vivo histopathological toxicity studies revealed that the DOX-micelles produced significant less cardiac damage than free DOX, while the in vivo immunohistochemical assays exhibited a significantly higher DNA damage in the cardiac tissue with the free DOX than with our DOX-micelles. All these results demonstrate that our novel nanotechnological platform could successfully deliver DOX to the tumor with reduced cardiotoxicity, thus showing promising clinical potential as an anticancer drug delivery carrier.
format Artículo
author Cagel, Maximiliano
Moretton, Marcela A.
Bernabeu, Ezequiel
Zubillaga, Marcelo
Lagomarsino, Eduardo
Vanzulli, Silvia
Nicoud, Melisa Beatriz
Medina, Vanina Araceli
Salgueiro, María J.
Chiappetta, Diego A.
author_facet Cagel, Maximiliano
Moretton, Marcela A.
Bernabeu, Ezequiel
Zubillaga, Marcelo
Lagomarsino, Eduardo
Vanzulli, Silvia
Nicoud, Melisa Beatriz
Medina, Vanina Araceli
Salgueiro, María J.
Chiappetta, Diego A.
author_sort Cagel, Maximiliano
title Antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4T1 murine breast cancer model. Comparative studies using Doxil® and free doxorubicin
title_short Antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4T1 murine breast cancer model. Comparative studies using Doxil® and free doxorubicin
title_full Antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4T1 murine breast cancer model. Comparative studies using Doxil® and free doxorubicin
title_fullStr Antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4T1 murine breast cancer model. Comparative studies using Doxil® and free doxorubicin
title_full_unstemmed Antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4T1 murine breast cancer model. Comparative studies using Doxil® and free doxorubicin
title_sort antitumor efficacy and cardiotoxic effect of doxorubicin-loaded mixed micelles in 4t1 murine breast cancer model. comparative studies using doxil® and free doxorubicin
publisher Elsevier
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14229
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