Fluorescence sensors for imaging membrane lipid domains and cholesterol

Abstract: Lipid membrane domains are supramolecular lateral heterogeneities of biological membranes. Of nanoscopic dimensions, they constitute specialized hubs used by the cell as transient signaling platforms for a great variety of biologically important mechanisms. Their property to form and diss...

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Detalles Bibliográficos
Autor principal: Barrantes, Francisco José
Formato: Parte de libro
Lenguaje:Inglés
Publicado: Academic Press 2022
Materias:
BIOSENSORES
COLESTEROL
MICROSCOPIO FLUORESCENTE
SONDAS FLUORESCENTES
DISTRIBUCIÓN EN EL PLANO
SIMETRÍA DE LÍPIDOS
DOMINIO LIPÍDICO
DOMINIOS DE MEMBRANA
NANOSCOPIA
SENSORES
MICROSCOPÍA DE SUPERRESOLUCIÓN
DISTRIBUCIÓN TRANSBICAPA
TINCIÓN VIRTUAL
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14078
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14078
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic BIOSENSORES
COLESTEROL
MICROSCOPIO FLUORESCENTE
SONDAS FLUORESCENTES
DISTRIBUCIÓN EN EL PLANO
SIMETRÍA DE LÍPIDOS
DOMINIO LIPÍDICO
DOMINIOS DE MEMBRANA
NANOSCOPIA
SENSORES
MICROSCOPÍA DE SUPERRESOLUCIÓN
DISTRIBUCIÓN TRANSBICAPA
TINCIÓN VIRTUAL
spellingShingle BIOSENSORES
COLESTEROL
MICROSCOPIO FLUORESCENTE
SONDAS FLUORESCENTES
DISTRIBUCIÓN EN EL PLANO
SIMETRÍA DE LÍPIDOS
DOMINIO LIPÍDICO
DOMINIOS DE MEMBRANA
NANOSCOPIA
SENSORES
MICROSCOPÍA DE SUPERRESOLUCIÓN
DISTRIBUCIÓN TRANSBICAPA
TINCIÓN VIRTUAL
Barrantes, Francisco José
Fluorescence sensors for imaging membrane lipid domains and cholesterol
topic_facet BIOSENSORES
COLESTEROL
MICROSCOPIO FLUORESCENTE
SONDAS FLUORESCENTES
DISTRIBUCIÓN EN EL PLANO
SIMETRÍA DE LÍPIDOS
DOMINIO LIPÍDICO
DOMINIOS DE MEMBRANA
NANOSCOPIA
SENSORES
MICROSCOPÍA DE SUPERRESOLUCIÓN
DISTRIBUCIÓN TRANSBICAPA
TINCIÓN VIRTUAL
description Abstract: Lipid membrane domains are supramolecular lateral heterogeneities of biological membranes. Of nanoscopic dimensions, they constitute specialized hubs used by the cell as transient signaling platforms for a great variety of biologically important mechanisms. Their property to form and dissolve in the bulk lipid bilayer endow them with the ability to engage in highly dynamic processes, and temporarily recruit subpopulations of membrane proteins in reduced nanometric compartments that can coalesce to form larger mesoscale assemblies. Cholesterol is an essential component of these lipid domains; its unique molecular structure is suitable for interacting intricately with crevices and cavities of transmembrane protein surfaces through its rough β face while "talking" to fatty acid acyl chains of glycerophospholipids and sphingolipids via its smooth α face. Progress in the field of membrane domains has been closely associated with innovative improvements in fluorescence microscopy and new fluorescence sensors. These advances enabled the exploration of the biophysical properties of lipids and their supramolecular platforms. Here I review the rationale behind the use of biosensors over the last few decades and their contributions towards elucidation of the in-plane and transbilayer topography of cholesterol-enriched lipid domains and their molecular constituents. The challenges introduced by super-resolution optical microscopy are discussed, as well as possible scenarios for future developments in the field, including virtual ("no staining") staining.
format Parte de libro
author Barrantes, Francisco José
author_facet Barrantes, Francisco José
author_sort Barrantes, Francisco José
title Fluorescence sensors for imaging membrane lipid domains and cholesterol
title_short Fluorescence sensors for imaging membrane lipid domains and cholesterol
title_full Fluorescence sensors for imaging membrane lipid domains and cholesterol
title_fullStr Fluorescence sensors for imaging membrane lipid domains and cholesterol
title_full_unstemmed Fluorescence sensors for imaging membrane lipid domains and cholesterol
title_sort fluorescence sensors for imaging membrane lipid domains and cholesterol
publisher Academic Press
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14078
work_keys_str_mv AT barrantesfranciscojose fluorescencesensorsforimagingmembranelipiddomainsandcholesterol
bdutipo_str Repositorios
_version_ 1764820523823398915

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