Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers

"A label-free molecular imprinted polymer (MIP) sensor was fabricated for the detection of progesterone in aqueous solutions, by polymerization inside the void spaces of colloidal crystals, which gave them photonic properties. The prepolymerization mixture was prepared from acrylic acid as the...

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Autores principales: Qasim, Sally, Hsu, Shu-Yu, Rossi, Ezequiel, Lin, Chung-Ho, Polo Parada, Luis, Fidalgo de Cortalezzi, María Marta, Salahshoor, Zahra
Formato: Artículos de Publicaciones Periódicas acceptedVersion
Lenguaje:Inglés
Publicado: info
Materias:
POLIMEROS
PROGESTERONA
Acceso en línea:https://ri.itba.edu.ar/handle/123456789/4009
Aporte de:
Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA) de Instituto Tecnológico de Buenos Aires (ITBA)
id I32-R138-123456789-4009
record_format dspace
spelling I32-R138-123456789-40092022-12-07T13:06:49Z Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers Qasim, Sally Hsu, Shu-Yu Rossi, Ezequiel Lin, Chung-Ho Polo Parada, Luis Fidalgo de Cortalezzi, María Marta Salahshoor, Zahra POLIMEROS PROGESTERONA "A label-free molecular imprinted polymer (MIP) sensor was fabricated for the detection of progesterone in aqueous solutions, by polymerization inside the void spaces of colloidal crystals, which gave them photonic properties. The prepolymerization mixture was prepared from acrylic acid as the functional monomer, ethylene glycol as the cross-linker agent, ethanol as solvent, and progesterone as the imprinted template. After polymerization, the colloidal crystal was removed by acid etching and the target eluted with a solvent. Material characterization included as follows: attenuated total reflectance-Fourier-transform infrared spectroscopy, dynamic light scattering, swelling experiments, and environmental scanning electron microscopy. MIPs were investigated by equilibrium binding, kinetics experiments, and UV–visible spectra to investigate Bragg diffraction peak shift that occurs with the rebinding at different progesterone concentrations in deionized water and 150-mM NaCl solutions. The MIP response was investigated with progesterone concentration in the 1–100 μg L−1 range, with LOD of 0.5 μg L−1, reaching the detected range of hormone in natural waters. Furthermore, hydrogel MIP films were successfully tested in various real water matrices with satisfactory results. Moreover, the MIP film exhibited good selectivity toward the progesterone hormone evidenced by a larger response than when exposed to structurally similar molecules. Computational studies suggested that size along with surface potential influenced the binding of analog compounds. Due to their ease of use and low cost, the sensors are promising as screening tools for the presence of progesterone in aqueous samples." info:eu-repo/date/embargoEnd/2023-04-02 2022-11-16T13:17:13Z 2022-11-16T13:17:13Z 2022-04 Artículos de Publicaciones Periódicas info:eu-repo/semantics/acceptedVersion 1436-5073 https://ri.itba.edu.ar/handle/123456789/4009 en info:eu-repo/semantics/altIdentifier/doi/10.1007/s00604-022-05290-w info:eu-repo/semantics/embargoedAccess application/pdf application/pdf
institution Instituto Tecnológico de Buenos Aires (ITBA)
institution_str I-32
repository_str R-138
collection Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA)
language Inglés
topic POLIMEROS
PROGESTERONA
spellingShingle POLIMEROS
PROGESTERONA
Qasim, Sally
Hsu, Shu-Yu
Rossi, Ezequiel
Lin, Chung-Ho
Polo Parada, Luis
Fidalgo de Cortalezzi, María Marta
Salahshoor, Zahra
Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
topic_facet POLIMEROS
PROGESTERONA
description "A label-free molecular imprinted polymer (MIP) sensor was fabricated for the detection of progesterone in aqueous solutions, by polymerization inside the void spaces of colloidal crystals, which gave them photonic properties. The prepolymerization mixture was prepared from acrylic acid as the functional monomer, ethylene glycol as the cross-linker agent, ethanol as solvent, and progesterone as the imprinted template. After polymerization, the colloidal crystal was removed by acid etching and the target eluted with a solvent. Material characterization included as follows: attenuated total reflectance-Fourier-transform infrared spectroscopy, dynamic light scattering, swelling experiments, and environmental scanning electron microscopy. MIPs were investigated by equilibrium binding, kinetics experiments, and UV–visible spectra to investigate Bragg diffraction peak shift that occurs with the rebinding at different progesterone concentrations in deionized water and 150-mM NaCl solutions. The MIP response was investigated with progesterone concentration in the 1–100 μg L−1 range, with LOD of 0.5 μg L−1, reaching the detected range of hormone in natural waters. Furthermore, hydrogel MIP films were successfully tested in various real water matrices with satisfactory results. Moreover, the MIP film exhibited good selectivity toward the progesterone hormone evidenced by a larger response than when exposed to structurally similar molecules. Computational studies suggested that size along with surface potential influenced the binding of analog compounds. Due to their ease of use and low cost, the sensors are promising as screening tools for the presence of progesterone in aqueous samples."
format Artículos de Publicaciones Periódicas
acceptedVersion
author Qasim, Sally
Hsu, Shu-Yu
Rossi, Ezequiel
Lin, Chung-Ho
Polo Parada, Luis
Fidalgo de Cortalezzi, María Marta
Salahshoor, Zahra
author_facet Qasim, Sally
Hsu, Shu-Yu
Rossi, Ezequiel
Lin, Chung-Ho
Polo Parada, Luis
Fidalgo de Cortalezzi, María Marta
Salahshoor, Zahra
author_sort Qasim, Sally
title Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
title_short Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
title_full Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
title_fullStr Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
title_full_unstemmed Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
title_sort detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
publishDate info
url https://ri.itba.edu.ar/handle/123456789/4009
work_keys_str_mv AT qasimsally detectionofprogesteroneinaqueoussamplesbymolecularlyimprintedphotonicpolymers
AT hsushuyu detectionofprogesteroneinaqueoussamplesbymolecularlyimprintedphotonicpolymers
AT rossiezequiel detectionofprogesteroneinaqueoussamplesbymolecularlyimprintedphotonicpolymers
AT linchungho detectionofprogesteroneinaqueoussamplesbymolecularlyimprintedphotonicpolymers
AT poloparadaluis detectionofprogesteroneinaqueoussamplesbymolecularlyimprintedphotonicpolymers
AT fidalgodecortalezzimariamarta detectionofprogesteroneinaqueoussamplesbymolecularlyimprintedphotonicpolymers
AT salahshoorzahra detectionofprogesteroneinaqueoussamplesbymolecularlyimprintedphotonicpolymers
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