Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization

"Imaging of fluorescent small molecule transport into electropermeabilized cells reveals polarized patterns of entry, which must reflect in some way the mechanisms of the migration of these molecules across the compromised membrane barrier. In some reports, transport occurs primarily across the...

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Autores principales: Sözer, Esin B., Pocetti, Florencia, Vernier, P. Thomas
Formato: Artículos de Publicaciones Periódicas publishedVersion
Lenguaje:Inglés
Publicado: 2019
Materias:
MOLECULAS
TRANSPORTE
ELECTROPORACION
MEMBRANAS
Acceso en línea:http://ri.itba.edu.ar/handle/123456789/1644
Aporte de:
Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA) de Instituto Tecnológico de Buenos Aires (ITBA)
id I32-R138-123456789-1644
record_format dspace
spelling I32-R138-123456789-16442022-12-07T13:05:48Z Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization Sözer, Esin B. Pocetti, Florencia Vernier, P. Thomas MOLECULAS TRANSPORTE ELECTROPORACION MEMBRANAS "Imaging of fluorescent small molecule transport into electropermeabilized cells reveals polarized patterns of entry, which must reflect in some way the mechanisms of the migration of these molecules across the compromised membrane barrier. In some reports, transport occurs primarily across the areas of the membrane nearest the positive electrode (anode), but in others cathode-facing entry dominates. Here we compare YO-PRO-1, propidium, and calcein uptake into U-937 cells after nanosecond (6 ns) and microsecond (220 ls) electric pulse exposures. Each of the three dyes exhibits a different pattern. Calcein shows no preference for anode- or cathode-facing entry that is detectable with our measurement system. Immediately after a microsecond pulse, YO-PRO-1 and propidium enter the cell roughly equally from the positive and negative poles, but transport through the cathode-facing side dominates in less than 1 s. After nanosecond pulse permeabilization, YO-PRO-1 and propidium enter primarily on the anode-facing side of the cell." 2019-07-11T20:23:20Z 2019-07-11T20:23:20Z 2018 Artículos de Publicaciones Periódicas info:eu-repo/semantics/publishedVersion 0022-2631 http://ri.itba.edu.ar/handle/123456789/1644 en info:eu-repo/semantics/reference/doi/10.1007/s00232-017-9962-1 info:eu-repo/grantAgreement/AFOSR/FA9550-14-1-0123/US. Arlington, VA. info:eu-repo/grantAgreement/AFOSR/MURI/FA9550-15-1-0517/US. Arlington, VA. info:eu-repo/grantAgreement/MECCyT/BEC.AR/AR.Ciudad Autónoma de Buenos Aires https://creativecommons.org/licenses/by/4.0/ application/pdf
institution Instituto Tecnológico de Buenos Aires (ITBA)
institution_str I-32
repository_str R-138
collection Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA)
language Inglés
topic MOLECULAS
TRANSPORTE
ELECTROPORACION
MEMBRANAS
spellingShingle MOLECULAS
TRANSPORTE
ELECTROPORACION
MEMBRANAS
Sözer, Esin B.
Pocetti, Florencia
Vernier, P. Thomas
Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
topic_facet MOLECULAS
TRANSPORTE
ELECTROPORACION
MEMBRANAS
description "Imaging of fluorescent small molecule transport into electropermeabilized cells reveals polarized patterns of entry, which must reflect in some way the mechanisms of the migration of these molecules across the compromised membrane barrier. In some reports, transport occurs primarily across the areas of the membrane nearest the positive electrode (anode), but in others cathode-facing entry dominates. Here we compare YO-PRO-1, propidium, and calcein uptake into U-937 cells after nanosecond (6 ns) and microsecond (220 ls) electric pulse exposures. Each of the three dyes exhibits a different pattern. Calcein shows no preference for anode- or cathode-facing entry that is detectable with our measurement system. Immediately after a microsecond pulse, YO-PRO-1 and propidium enter the cell roughly equally from the positive and negative poles, but transport through the cathode-facing side dominates in less than 1 s. After nanosecond pulse permeabilization, YO-PRO-1 and propidium enter primarily on the anode-facing side of the cell."
format Artículos de Publicaciones Periódicas
publishedVersion
author Sözer, Esin B.
Pocetti, Florencia
Vernier, P. Thomas
author_facet Sözer, Esin B.
Pocetti, Florencia
Vernier, P. Thomas
author_sort Sözer, Esin B.
title Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
title_short Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
title_full Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
title_fullStr Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
title_full_unstemmed Asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
title_sort asymmetric patterns of small molecule transport after nanosecond and microsecond electropermeabilization
publishDate 2019
url http://ri.itba.edu.ar/handle/123456789/1644
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AT pocettiflorencia asymmetricpatternsofsmallmoleculetransportafternanosecondandmicrosecondelectropermeabilization
AT vernierpthomas asymmetricpatternsofsmallmoleculetransportafternanosecondandmicrosecondelectropermeabilization
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