Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine

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The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35...

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Autores principales:	Cardillo, S.B., Moretti, M.B., García, S.C.
Formato:	Artículo publishedVersion
Publicado:	2010
Materias:	4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae protein binding article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region Saccharomyces cerevisiae drug effects DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15359778_v9_n8_p1262_Cardillo https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_15359778_v9_n8_p1262_Cardillo_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_15359778_v9_n8_p1262_Cardillo_oai
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spelling	I28-R145-paper_15359778_v9_n8_p1262_Cardillo_oai2024-08-16 Cardillo, S.B. Moretti, M.B. García, S.C. 2010 The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35/Dal81. UGA4 is subjected to a very complex regulation, and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that the Uga3 and Uga35/Dal81 transcription factors bind to the UGA4 promoter in a GABA-dependent manner and that this binding is impaired by the presence of leucine. We also found that the Leu3 transcription factor negatively regulates UGA4 transcription, although this seems to be through an indirect mechanism. © 2010, American Society for Microbiology. Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_15359778_v9_n8_p1262_Cardillo info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Eukaryotic Cell 2010;9(8):1262-1271 4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_15359778_v9_n8_p1262_Cardillo_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic
spellingShingle	4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Cardillo, S.B. Moretti, M.B. García, S.C. Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine
topic_facet	4 aminobutyric acid 4 aminobutyric acid carrier DAL81 protein, S cerevisiae DNA binding protein leucine Saccharomyces cerevisiae protein transcription factor UGA3 protein, S cerevisiae UGA4 protein, S cerevisiae 4 aminobutyric acid 4 aminobutyric acid carrier DNA binding protein leucine protein binding Saccharomyces cerevisiae protein transcription factor article biological model drug effect fungal gene gene expression regulation genetic transcription genetics metabolism mutation promoter region protein binding Saccharomyces cerevisiae drug effects genetic transcription genetics metabolism Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae DNA-Binding Proteins GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Fungal Genes, Fungal Leucine Models, Genetic Mutation Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic
description	The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35/Dal81. UGA4 is subjected to a very complex regulation, and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that the Uga3 and Uga35/Dal81 transcription factors bind to the UGA4 promoter in a GABA-dependent manner and that this binding is impaired by the presence of leucine. We also found that the Leu3 transcription factor negatively regulates UGA4 transcription, although this seems to be through an indirect mechanism. © 2010, American Society for Microbiology.
format	Artículo Artículo publishedVersion
author	Cardillo, S.B. Moretti, M.B. García, S.C.
author_facet	Cardillo, S.B. Moretti, M.B. García, S.C.
author_sort	Cardillo, S.B.
title	Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine
title_short	Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine
title_full	Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine
title_fullStr	Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine
title_full_unstemmed	Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine
title_sort	uga3 and uga35/dal81 transcription factors regulate uga4 transcription in response to γ-aminobutyric acid and leucine
publishDate	2010
url	http://hdl.handle.net/20.500.12110/paper_15359778_v9_n8_p1262_Cardillo https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_15359778_v9_n8_p1262_Cardillo_oai
work_keys_str_mv	AT cardillosb uga3anduga35dal81transcriptionfactorsregulateuga4transcriptioninresponsetogaminobutyricacidandleucine AT morettimb uga3anduga35dal81transcriptionfactorsregulateuga4transcriptioninresponsetogaminobutyricacidandleucine AT garciasc uga3anduga35dal81transcriptionfactorsregulateuga4transcriptioninresponsetogaminobutyricacidandleucine
_version_	1809356929742405632

Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine

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