Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | Artículo publishedVersion |
| Publicado: |
2009
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14248220_v9_n3_p1599_Kormes https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_14248220_v9_n3_p1599_Kormes_oai |
| Aporte de: |
| id |
I28-R145-paper_14248220_v9_n3_p1599_Kormes_oai |
|---|---|
| record_format |
dspace |
| spelling |
I28-R145-paper_14248220_v9_n3_p1599_Kormes_oai2024-08-16 Kormes, D.J. Cortón, E. 2009 Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed. © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_14248220_v9_n3_p1599_Kormes info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Sensors 2009;9(3):1599-1608 Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_14248220_v9_n3_p1599_Kormes_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors |
| spellingShingle |
Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors Kormes, D.J. Cortón, E. Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach |
| topic_facet |
Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors |
| description |
Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed. © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. |
| format |
Artículo Artículo publishedVersion |
| author |
Kormes, D.J. Cortón, E. |
| author_facet |
Kormes, D.J. Cortón, E. |
| author_sort |
Kormes, D.J. |
| title |
Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach |
| title_short |
Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach |
| title_full |
Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach |
| title_fullStr |
Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach |
| title_full_unstemmed |
Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach |
| title_sort |
development of a novel method for in vivo determination of activation energy of glucose transport across s. cerevisiae cellular membranes. a biosensor-like approach |
| publishDate |
2009 |
| url |
http://hdl.handle.net/20.500.12110/paper_14248220_v9_n3_p1599_Kormes https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_14248220_v9_n3_p1599_Kormes_oai |
| work_keys_str_mv |
AT kormesdj developmentofanovelmethodforinvivodeterminationofactivationenergyofglucosetransportacrossscerevisiaecellularmembranesabiosensorlikeapproach AT cortone developmentofanovelmethodforinvivodeterminationofactivationenergyofglucosetransportacrossscerevisiaecellularmembranesabiosensorlikeapproach |
| _version_ |
1809357235151699968 |