Cell wall modifications during osmotic stress in Lactobacillus casei

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Aims: To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. Methods and Results: Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l -1 NaCl; N condition) and control (MRS; C condition) conditions were deter...

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Autores principales:	Piuri, M., Sanchez-Rivas, C., Ruzal, S.M.
Formato:	Artículo publishedVersion
Publicado:	2005
Materias:	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Microscopy, Electron Osmosis Penicillin-Binding Proteins Bacteria (microorganisms) Lactobacillus Posibacteria
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13645072_v98_n1_p84_Piuri http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_13645072_v98_n1_p84_Piuri_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_13645072_v98_n1_p84_Piuri_oai
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spelling	I28-R145-paper_13645072_v98_n1_p84_Piuri_oai2020-10-19 Piuri, M. Sanchez-Rivas, C. Ruzal, S.M. 2005 Aims: To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. Methods and Results: Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l -1 NaCl; N condition) and control (MRS; C condition) conditions were determined by transmission electronic microscopy and analytical procedures. Lactobacillus casei cells grown in N condition were significantly larger than cells grown under unstressed C condition. Increased sensitivity to mutanolysin and antibiotics with target in the cell wall was observed in N condition. Purified cell wall also showed the increased sensitivity to lysis by mutanolysin. Analysis of peptidoglycan (PG) from stressed cells showed that modification was at the structural level in accordance with a decreased PG cross-link involving penicillin-binding proteins (PBP). Nine PBP were first described in this species and these proteins were expressed in low percentages or presented a modified pattern of saturation with penicillin G (Pen G) during growth in high salt. Three of the essential PBP were fully saturated in N condition at lower Pen G concentrations than in C condition, suggesting differences in functionality in vivo. Conclusions: The results show that growth in high salt modified the structural properties of the cell wall. Significance and Impact of Study: Advances in understanding the adaptation to high osmolarity, in particular those involving sensitivity to lysis of lactic acid bacteria. Fil:Piuri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_13645072_v98_n1_p84_Piuri info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Appl. Microbiol. 2005;98(1):84-95 Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria Cell wall modifications during osmotic stress in Lactobacillus casei info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_13645072_v98_n1_p84_Piuri_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria
spellingShingle	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria Piuri, M. Sanchez-Rivas, C. Ruzal, S.M. Cell wall modifications during osmotic stress in Lactobacillus casei
topic_facet	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria
description	Aims: To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. Methods and Results: Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l -1 NaCl; N condition) and control (MRS; C condition) conditions were determined by transmission electronic microscopy and analytical procedures. Lactobacillus casei cells grown in N condition were significantly larger than cells grown under unstressed C condition. Increased sensitivity to mutanolysin and antibiotics with target in the cell wall was observed in N condition. Purified cell wall also showed the increased sensitivity to lysis by mutanolysin. Analysis of peptidoglycan (PG) from stressed cells showed that modification was at the structural level in accordance with a decreased PG cross-link involving penicillin-binding proteins (PBP). Nine PBP were first described in this species and these proteins were expressed in low percentages or presented a modified pattern of saturation with penicillin G (Pen G) during growth in high salt. Three of the essential PBP were fully saturated in N condition at lower Pen G concentrations than in C condition, suggesting differences in functionality in vivo. Conclusions: The results show that growth in high salt modified the structural properties of the cell wall. Significance and Impact of Study: Advances in understanding the adaptation to high osmolarity, in particular those involving sensitivity to lysis of lactic acid bacteria.
format	Artículo Artículo publishedVersion
author	Piuri, M. Sanchez-Rivas, C. Ruzal, S.M.
author_facet	Piuri, M. Sanchez-Rivas, C. Ruzal, S.M.
author_sort	Piuri, M.
title	Cell wall modifications during osmotic stress in Lactobacillus casei
title_short	Cell wall modifications during osmotic stress in Lactobacillus casei
title_full	Cell wall modifications during osmotic stress in Lactobacillus casei
title_fullStr	Cell wall modifications during osmotic stress in Lactobacillus casei
title_full_unstemmed	Cell wall modifications during osmotic stress in Lactobacillus casei
title_sort	cell wall modifications during osmotic stress in lactobacillus casei
publishDate	2005
url	http://hdl.handle.net/20.500.12110/paper_13645072_v98_n1_p84_Piuri http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_13645072_v98_n1_p84_Piuri_oai
work_keys_str_mv	AT piurim cellwallmodificationsduringosmoticstressinlactobacilluscasei AT sanchezrivasc cellwallmodificationsduringosmoticstressinlactobacilluscasei AT ruzalsm cellwallmodificationsduringosmoticstressinlactobacilluscasei
_version_	1766026759797473280

Cell wall modifications during osmotic stress in Lactobacillus casei

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