A slow RNA polymerase II affects alternative splicing in vivo

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Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation...

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Autores principales:	De La Mata, M., Alonso, C.R., Kadener, S., Fededa, J.P., Blaustein, M., Pelisch, F., Cramer, P., Bentley, D., Kornblihtt, A.R.
Formato:	Artículo publishedVersion
Publicado:	2003
Materias:	elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10972765_v12_n2_p525_DeLaMata_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_10972765_v12_n2_p525_DeLaMata_oai
record_format	dspace
spelling	I28-R145-paper_10972765_v12_n2_p525_DeLaMata_oai2020-10-19 De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R. 2003 Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo. Fil:De La Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alonso, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Mol. Cell 2003;12(2):525-532 elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae A slow RNA polymerase II affects alternative splicing in vivo info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10972765_v12_n2_p525_DeLaMata_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae
spellingShingle	elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R. A slow RNA polymerase II affects alternative splicing in vivo
topic_facet	elongation factor fibronectin protein subunit RNA RNA polymerase II Adenovirus article cell culture Drosophila embryo cell exon gene genetic transcription genetic transfection Hox gene human in vivo study point mutation protein expression reverse transcription polymerase chain reaction RNA splicing RNA translation Western blotting Adenoviridae
description	Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.
format	Artículo Artículo publishedVersion
author	De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R.
author_facet	De La Mata, M. Alonso, C.R. Kadener, S. Fededa, J.P. Blaustein, M. Pelisch, F. Cramer, P. Bentley, D. Kornblihtt, A.R.
author_sort	De La Mata, M.
title	A slow RNA polymerase II affects alternative splicing in vivo
title_short	A slow RNA polymerase II affects alternative splicing in vivo
title_full	A slow RNA polymerase II affects alternative splicing in vivo
title_fullStr	A slow RNA polymerase II affects alternative splicing in vivo
title_full_unstemmed	A slow RNA polymerase II affects alternative splicing in vivo
title_sort	slow rna polymerase ii affects alternative splicing in vivo
publishDate	2003
url	http://hdl.handle.net/20.500.12110/paper_10972765_v12_n2_p525_DeLaMata http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10972765_v12_n2_p525_DeLaMata_oai
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A slow RNA polymerase II affects alternative splicing in vivo

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