UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix

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Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spect...

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Autores principales:	Landoni, M., Duschak, V.G., Erra-Balsells, R., Couto, A.S.
Formato:	Artículo publishedVersion
Publicado:	2008
Materias:	Amides Computer networks Desorption Lipids Mass spectrometers Mass spectrometry Matrix algebra Microfluidics Network protocols Pulsed laser deposition Spectrometers Spectrometry Spectrum analysis American society Analyte ions Ceramide Epimastigote Glycosphingolipids (GSL) High quality (HQ) MALDI-MS Mass spectrometry analysis Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Matrix-assisted laser-desorption/ionization (MALDI) Molecular processes new applications Sphingolipids Trypanosoma cruzi Ultra-violet (UV) High performance liquid chromatography acetylceramide ceramide ceramide derivative fluorescent dye galactosylceramide galactosyldihydroceramide glucosylceramide glycosphingolipid ion nitrobenz 2 oxa 1,3 diazole pyrrole derivative article calibration covalent bond epimastigote instrumentation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman signal detection Animals Glycosphingolipids Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Sphingoidea
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10440305_v19_n7_p923_Landoni http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v19_n7_p923_Landoni_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_10440305_v19_n7_p923_Landoni_oai
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spelling	I28-R145-paper_10440305_v19_n7_p923_Landoni_oai2020-10-19 Landoni, M. Duschak, V.G. Erra-Balsells, R. Couto, A.S. 2008 Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals. © 2008 American Society for Mass Spectrometry. Fil:Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_10440305_v19_n7_p923_Landoni info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Am. Soc. Mass Spectrom. 2008;19(7):923-926 Amides Computer networks Desorption Lipids Mass spectrometers Mass spectrometry Matrix algebra Microfluidics Network protocols Pulsed laser deposition Spectrometers Spectrometry Spectrum analysis American society Analyte ions Ceramide Epimastigote Glycosphingolipids (GSL) High quality (HQ) MALDI-MS Mass spectrometry analysis Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Matrix-assisted laser-desorption/ionization (MALDI) Molecular processes new applications Sphingolipids Trypanosoma cruzi Ultra-violet (UV) High performance liquid chromatography acetylceramide ceramide ceramide derivative fluorescent dye galactosylceramide galactosyldihydroceramide glucosylceramide glycosphingolipid ion nitrobenz 2 oxa 1,3 diazole pyrrole derivative article calibration covalent bond epimastigote instrumentation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman signal detection Trypanosoma cruzi Animals Glycosphingolipids Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Trypanosoma cruzi Sphingoidea Trypanosoma cruzi UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v19_n7_p923_Landoni_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Amides Computer networks Desorption Lipids Mass spectrometers Mass spectrometry Matrix algebra Microfluidics Network protocols Pulsed laser deposition Spectrometers Spectrometry Spectrum analysis American society Analyte ions Ceramide Epimastigote Glycosphingolipids (GSL) High quality (HQ) MALDI-MS Mass spectrometry analysis Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Matrix-assisted laser-desorption/ionization (MALDI) Molecular processes new applications Sphingolipids Trypanosoma cruzi Ultra-violet (UV) High performance liquid chromatography acetylceramide ceramide ceramide derivative fluorescent dye galactosylceramide galactosyldihydroceramide glucosylceramide glycosphingolipid ion nitrobenz 2 oxa 1,3 diazole pyrrole derivative article calibration covalent bond epimastigote instrumentation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman signal detection Trypanosoma cruzi Animals Glycosphingolipids Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Trypanosoma cruzi Sphingoidea Trypanosoma cruzi
spellingShingle	Amides Computer networks Desorption Lipids Mass spectrometers Mass spectrometry Matrix algebra Microfluidics Network protocols Pulsed laser deposition Spectrometers Spectrometry Spectrum analysis American society Analyte ions Ceramide Epimastigote Glycosphingolipids (GSL) High quality (HQ) MALDI-MS Mass spectrometry analysis Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Matrix-assisted laser-desorption/ionization (MALDI) Molecular processes new applications Sphingolipids Trypanosoma cruzi Ultra-violet (UV) High performance liquid chromatography acetylceramide ceramide ceramide derivative fluorescent dye galactosylceramide galactosyldihydroceramide glucosylceramide glycosphingolipid ion nitrobenz 2 oxa 1,3 diazole pyrrole derivative article calibration covalent bond epimastigote instrumentation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman signal detection Trypanosoma cruzi Animals Glycosphingolipids Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Trypanosoma cruzi Sphingoidea Trypanosoma cruzi Landoni, M. Duschak, V.G. Erra-Balsells, R. Couto, A.S. UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix
topic_facet	Amides Computer networks Desorption Lipids Mass spectrometers Mass spectrometry Matrix algebra Microfluidics Network protocols Pulsed laser deposition Spectrometers Spectrometry Spectrum analysis American society Analyte ions Ceramide Epimastigote Glycosphingolipids (GSL) High quality (HQ) MALDI-MS Mass spectrometry analysis Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Matrix-assisted laser-desorption/ionization (MALDI) Molecular processes new applications Sphingolipids Trypanosoma cruzi Ultra-violet (UV) High performance liquid chromatography acetylceramide ceramide ceramide derivative fluorescent dye galactosylceramide galactosyldihydroceramide glucosylceramide glycosphingolipid ion nitrobenz 2 oxa 1,3 diazole pyrrole derivative article calibration covalent bond epimastigote instrumentation mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman signal detection Trypanosoma cruzi Animals Glycosphingolipids Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Trypanosoma cruzi Sphingoidea Trypanosoma cruzi
description	Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals. © 2008 American Society for Mass Spectrometry.
format	Artículo Artículo publishedVersion
author	Landoni, M. Duschak, V.G. Erra-Balsells, R. Couto, A.S.
author_facet	Landoni, M. Duschak, V.G. Erra-Balsells, R. Couto, A.S.
author_sort	Landoni, M.
title	UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix
title_short	UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix
title_full	UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix
title_fullStr	UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix
title_full_unstemmed	UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix
title_sort	uv-maldi mass spectrometry analysis of nbd-glycosphingolipids without an external matrix
publishDate	2008
url	http://hdl.handle.net/20.500.12110/paper_10440305_v19_n7_p923_Landoni http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v19_n7_p923_Landoni_oai
work_keys_str_mv	AT landonim uvmaldimassspectrometryanalysisofnbdglycosphingolipidswithoutanexternalmatrix AT duschakvg uvmaldimassspectrometryanalysisofnbdglycosphingolipidswithoutanexternalmatrix AT errabalsellsr uvmaldimassspectrometryanalysisofnbdglycosphingolipidswithoutanexternalmatrix AT coutoas uvmaldimassspectrometryanalysisofnbdglycosphingolipidswithoutanexternalmatrix
_version_	1766026731872845824

UV-MALDI Mass Spectrometry Analysis of NBD-Glycosphingolipids Without an External Matrix

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