The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida

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To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code...

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Autores principales:	Raiger-Iustman, L.J., Ruiz, J.A.
Formato:	Artículo publishedVersion
Publicado:	2008
Materias:	Polyhydroxyalkanoate Pseudomonas putida Regulation RpoS Stress resistance bacterial enzyme polyhydroxyalkanoate depolymerase polyhydroxyalkanoate synthase polyhydroxyalkanoate synthase 1 polyhydroxyalkanoic acid sigma factor sigma factor RpoS article bacterial metabolism bacterial strain bacterial survival controlled study degradation gene inactivation nonhuman oxidative stress priority journal promoter region protein expression Bacterial Proteins Cloning, Molecular Gene Expression Regulation, Bacterial Gene Silencing Hydrogen Peroxide Microbial Viability Oxidative Stress Plasmids Polyhydroxyalkanoates Promoter Regions (Genetics) Recombinant Fusion Proteins Sigma Factor Time
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03781097_v284_n2_p218_RaigerIustman https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v284_n2_p218_RaigerIustman_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-paper_03781097_v284_n2_p218_RaigerIustman_oai2024-08-16 Raiger-Iustman, L.J. Ruiz, J.A. 2008 To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). By comparison with the wild-type, the rpoS mutant showed a higher polyhydroxyalkanoate degradation rate and increased expression of the translational fusion during the stationary growth phase. These results suggest that σS might control the genes involved in polyhydroxyalkanoate metabolism, possibly in an indirect manner. In addition, survival and oxidative stress assays performed under polyhydroxyalkanoate- and nonpolyhydroxyalkanoate- accumulating conditions demonstrated that the accumulated polyhydroxyalkanoate increased the survival and stress tolerance of the rpoS mutant. According to this, polyhydroxyalkanoate accumulation would help cells to overcome the adverse conditions encountered during the stationary phase in the strain that lacks RpoS. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. Fil:Raiger-Iustman, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_03781097_v284_n2_p218_RaigerIustman info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar FEMS Microbiol. Lett. 2008;284(2):218-224 Polyhydroxyalkanoate Pseudomonas putida Regulation RpoS Stress resistance bacterial enzyme polyhydroxyalkanoate depolymerase polyhydroxyalkanoate synthase polyhydroxyalkanoate synthase 1 polyhydroxyalkanoic acid sigma factor sigma factor RpoS article bacterial metabolism bacterial strain bacterial survival controlled study degradation gene inactivation nonhuman oxidative stress priority journal promoter region protein expression Pseudomonas putida Bacterial Proteins Cloning, Molecular Gene Expression Regulation, Bacterial Gene Silencing Hydrogen Peroxide Microbial Viability Oxidative Stress Plasmids Polyhydroxyalkanoates Promoter Regions (Genetics) Pseudomonas putida Recombinant Fusion Proteins Sigma Factor Time Pseudomonas putida The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v284_n2_p218_RaigerIustman_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Polyhydroxyalkanoate Pseudomonas putida Regulation RpoS Stress resistance bacterial enzyme polyhydroxyalkanoate depolymerase polyhydroxyalkanoate synthase polyhydroxyalkanoate synthase 1 polyhydroxyalkanoic acid sigma factor sigma factor RpoS article bacterial metabolism bacterial strain bacterial survival controlled study degradation gene inactivation nonhuman oxidative stress priority journal promoter region protein expression Pseudomonas putida Bacterial Proteins Cloning, Molecular Gene Expression Regulation, Bacterial Gene Silencing Hydrogen Peroxide Microbial Viability Oxidative Stress Plasmids Polyhydroxyalkanoates Promoter Regions (Genetics) Pseudomonas putida Recombinant Fusion Proteins Sigma Factor Time Pseudomonas putida
spellingShingle	Polyhydroxyalkanoate Pseudomonas putida Regulation RpoS Stress resistance bacterial enzyme polyhydroxyalkanoate depolymerase polyhydroxyalkanoate synthase polyhydroxyalkanoate synthase 1 polyhydroxyalkanoic acid sigma factor sigma factor RpoS article bacterial metabolism bacterial strain bacterial survival controlled study degradation gene inactivation nonhuman oxidative stress priority journal promoter region protein expression Pseudomonas putida Bacterial Proteins Cloning, Molecular Gene Expression Regulation, Bacterial Gene Silencing Hydrogen Peroxide Microbial Viability Oxidative Stress Plasmids Polyhydroxyalkanoates Promoter Regions (Genetics) Pseudomonas putida Recombinant Fusion Proteins Sigma Factor Time Pseudomonas putida Raiger-Iustman, L.J. Ruiz, J.A. The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
topic_facet	Polyhydroxyalkanoate Pseudomonas putida Regulation RpoS Stress resistance bacterial enzyme polyhydroxyalkanoate depolymerase polyhydroxyalkanoate synthase polyhydroxyalkanoate synthase 1 polyhydroxyalkanoic acid sigma factor sigma factor RpoS article bacterial metabolism bacterial strain bacterial survival controlled study degradation gene inactivation nonhuman oxidative stress priority journal promoter region protein expression Pseudomonas putida Bacterial Proteins Cloning, Molecular Gene Expression Regulation, Bacterial Gene Silencing Hydrogen Peroxide Microbial Viability Oxidative Stress Plasmids Polyhydroxyalkanoates Promoter Regions (Genetics) Pseudomonas putida Recombinant Fusion Proteins Sigma Factor Time Pseudomonas putida
description	To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). By comparison with the wild-type, the rpoS mutant showed a higher polyhydroxyalkanoate degradation rate and increased expression of the translational fusion during the stationary growth phase. These results suggest that σS might control the genes involved in polyhydroxyalkanoate metabolism, possibly in an indirect manner. In addition, survival and oxidative stress assays performed under polyhydroxyalkanoate- and nonpolyhydroxyalkanoate- accumulating conditions demonstrated that the accumulated polyhydroxyalkanoate increased the survival and stress tolerance of the rpoS mutant. According to this, polyhydroxyalkanoate accumulation would help cells to overcome the adverse conditions encountered during the stationary phase in the strain that lacks RpoS. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
format	Artículo Artículo publishedVersion
author	Raiger-Iustman, L.J. Ruiz, J.A.
author_facet	Raiger-Iustman, L.J. Ruiz, J.A.
author_sort	Raiger-Iustman, L.J.
title	The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
title_short	The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
title_full	The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
title_fullStr	The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
title_full_unstemmed	The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida
title_sort	alternative sigma factor, σs, affects polyhydroxyalkanoate metabolism in pseudomonas putida
publishDate	2008
url	http://hdl.handle.net/20.500.12110/paper_03781097_v284_n2_p218_RaigerIustman https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v284_n2_p218_RaigerIustman_oai
work_keys_str_mv	AT raigeriustmanlj thealternativesigmafactorssaffectspolyhydroxyalkanoatemetabolisminpseudomonasputida AT ruizja thealternativesigmafactorssaffectspolyhydroxyalkanoatemetabolisminpseudomonasputida AT raigeriustmanlj alternativesigmafactorssaffectspolyhydroxyalkanoatemetabolisminpseudomonasputida AT ruizja alternativesigmafactorssaffectspolyhydroxyalkanoatemetabolisminpseudomonasputida
_version_	1809356819649265664

The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida

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