Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and...
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1996
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03279545_v20_n3_p265_AldanaMarcos_oai |
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I28-R145-paper_03279545_v20_n3_p265_AldanaMarcos_oai2024-08-16 Aldana Marcos, H.J. Ferrari, C.C. Benitez, I. Affanni, J.M. 1996 This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories. Fil:Aldana Marcos, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrari, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Biocell 1996;20(3):265-272 Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates Standardization of fixation, processing and staining methods for the central nervous system of vertebrates info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03279545_v20_n3_p265_AldanaMarcos_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates |
| spellingShingle |
Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates Aldana Marcos, H.J. Ferrari, C.C. Benitez, I. Affanni, J.M. Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| topic_facet |
Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates |
| description |
This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories. |
| format |
Artículo Artículo publishedVersion |
| author |
Aldana Marcos, H.J. Ferrari, C.C. Benitez, I. Affanni, J.M. |
| author_facet |
Aldana Marcos, H.J. Ferrari, C.C. Benitez, I. Affanni, J.M. |
| author_sort |
Aldana Marcos, H.J. |
| title |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_short |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_full |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_fullStr |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_full_unstemmed |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_sort |
standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| publishDate |
1996 |
| url |
http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03279545_v20_n3_p265_AldanaMarcos_oai |
| work_keys_str_mv |
AT aldanamarcoshj standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates AT ferraricc standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates AT benitezi standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates AT affannijm standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates |
| _version_ |
1809357097087795200 |