Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages

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Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of...

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Autores principales:	Rodríguez, M.V., Mendiondo, G.M., Maskin, L., Gudesblat, G.E., Iusem, N.D., Benech-Arnold, R.L.
Formato:	Artículo publishedVersion
Publicado:	2009
Materias:	ABI5 Abscisic acid Hormone signalling Pre-harvest sprouting Seed dormancy Sorghum bicolor abscisic acid phytohormone signal transducing adaptor protein vegetable protein developmental stage embryo gene expression harvesting hormone imbibition inhibition intraspecific variation protein seed dormancy sorghum article gene expression regulation genetics germination growth, development and aging physiology plant gene plant seed polymerase chain reaction Western blotting Abscisic Acid Adaptor Proteins, Signal Transducing Blotting, Western Gene Expression Regulation, Plant Genes, Plant Germination Plant Growth Regulators Plant Proteins Polymerase Chain Reaction Seeds Sorghum Sorghum bicolor bicolor
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03057364_v104_n5_p975_Rodriguez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03057364_v104_n5_p975_Rodriguez_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_03057364_v104_n5_p975_Rodriguez_oai
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spelling	I28-R145-paper_03057364_v104_n5_p975_Rodriguez_oai2024-08-16 Rodríguez, M.V. Mendiondo, G.M. Maskin, L. Gudesblat, G.E. Iusem, N.D. Benech-Arnold, R.L. 2009 Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed. Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mendiondo, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_03057364_v104_n5_p975_Rodriguez info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Ann. Bot. 2009;104(5):975-985 ABI5 Abscisic acid Hormone signalling Pre-harvest sprouting Seed dormancy Sorghum bicolor abscisic acid phytohormone signal transducing adaptor protein vegetable protein abscisic acid developmental stage embryo gene expression harvesting hormone imbibition inhibition intraspecific variation protein seed dormancy sorghum article gene expression regulation genetics germination growth, development and aging physiology plant gene plant seed polymerase chain reaction sorghum Western blotting Abscisic Acid Adaptor Proteins, Signal Transducing Blotting, Western Gene Expression Regulation, Plant Genes, Plant Germination Plant Growth Regulators Plant Proteins Polymerase Chain Reaction Seeds Sorghum Sorghum bicolor Sorghum bicolor bicolor Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03057364_v104_n5_p975_Rodriguez_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	ABI5 Abscisic acid Hormone signalling Pre-harvest sprouting Seed dormancy Sorghum bicolor abscisic acid phytohormone signal transducing adaptor protein vegetable protein abscisic acid developmental stage embryo gene expression harvesting hormone imbibition inhibition intraspecific variation protein seed dormancy sorghum article gene expression regulation genetics germination growth, development and aging physiology plant gene plant seed polymerase chain reaction sorghum Western blotting Abscisic Acid Adaptor Proteins, Signal Transducing Blotting, Western Gene Expression Regulation, Plant Genes, Plant Germination Plant Growth Regulators Plant Proteins Polymerase Chain Reaction Seeds Sorghum Sorghum bicolor Sorghum bicolor bicolor
spellingShingle	ABI5 Abscisic acid Hormone signalling Pre-harvest sprouting Seed dormancy Sorghum bicolor abscisic acid phytohormone signal transducing adaptor protein vegetable protein abscisic acid developmental stage embryo gene expression harvesting hormone imbibition inhibition intraspecific variation protein seed dormancy sorghum article gene expression regulation genetics germination growth, development and aging physiology plant gene plant seed polymerase chain reaction sorghum Western blotting Abscisic Acid Adaptor Proteins, Signal Transducing Blotting, Western Gene Expression Regulation, Plant Genes, Plant Germination Plant Growth Regulators Plant Proteins Polymerase Chain Reaction Seeds Sorghum Sorghum bicolor Sorghum bicolor bicolor Rodríguez, M.V. Mendiondo, G.M. Maskin, L. Gudesblat, G.E. Iusem, N.D. Benech-Arnold, R.L. Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
topic_facet	ABI5 Abscisic acid Hormone signalling Pre-harvest sprouting Seed dormancy Sorghum bicolor abscisic acid phytohormone signal transducing adaptor protein vegetable protein abscisic acid developmental stage embryo gene expression harvesting hormone imbibition inhibition intraspecific variation protein seed dormancy sorghum article gene expression regulation genetics germination growth, development and aging physiology plant gene plant seed polymerase chain reaction sorghum Western blotting Abscisic Acid Adaptor Proteins, Signal Transducing Blotting, Western Gene Expression Regulation, Plant Genes, Plant Germination Plant Growth Regulators Plant Proteins Polymerase Chain Reaction Seeds Sorghum Sorghum bicolor Sorghum bicolor bicolor
description	Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.
format	Artículo Artículo publishedVersion
author	Rodríguez, M.V. Mendiondo, G.M. Maskin, L. Gudesblat, G.E. Iusem, N.D. Benech-Arnold, R.L.
author_facet	Rodríguez, M.V. Mendiondo, G.M. Maskin, L. Gudesblat, G.E. Iusem, N.D. Benech-Arnold, R.L.
author_sort	Rodríguez, M.V.
title	Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_short	Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_full	Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_fullStr	Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_full_unstemmed	Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_sort	expression of aba signalling genes and abi5 protein levels in imbibed sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
publishDate	2009
url	http://hdl.handle.net/20.500.12110/paper_03057364_v104_n5_p975_Rodriguez https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03057364_v104_n5_p975_Rodriguez_oai
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_version_	1809356816250830848

Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages

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