Developmental regulation of nicotinic synapses on cochlear inner hair cells
In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that...
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2004
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02706474_v24_n36_p7814_Katz http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_02706474_v24_n36_p7814_Katz_oai |
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I28-R145-paper_02706474_v24_n36_p7814_Katz_oai2020-10-19 Katz, E. Elgoyhen, A.B. Gómez-Casati, M.E. Knipper, M. Vetter, D.E. Fuchs, P.A. Glowatzki, E. 2004 In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that may contain both α9 and α10 subunits. Calcium influx through that receptor activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse is thought to disappear after the onset of hearing [after postnatal day 12 (P12)]. We documented this developmental transition using whole-cell recordings from IHCs in apical turns of the rat organ of Corti. Acetylcholine elicited ionic currents in 88-100% of IHCs between P3 and P14, but in only 1 of 11 IHCs at P16-P22. Potassium depolarization of efferent terminals caused IPSCs in 67% of IHCs at P3, in 100% at P7-P9, in 93% at P10-P12, but in only 40% at P13-P14 and in none of the IHCs tested between P16 and P22. Earlier work had shown by in situ hybridization that α9 mRNA is expressed in adult IHCs but that α10 mRNA disappears after the onset of hearing. In the present study, antibodies to α10 and to the associated calcium-dependent (SK2) potassium channel showed a similar developmental loss. The correlated expression of these gene products with functional innervation suggests that Alpha10 and SK2, but not Alpha9, are regulated by synaptic activity. Furthermore, this developmental knock-out of α10, but not α9, supports the hypothesis that functional nicotinic acetylcholine receptors in hair cells are heteromers containing both these subunits. Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gómez-Casati, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_02706474_v24_n36_p7814_Katz info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Neurosci. 2004;24(36):7814-7820 α9α10 nAChR Ca2+-activated K+ channel Cholinergic Efferent innervation IHC Mammalian cochlea Neonatal development Transient synapse acetylcholine calcium calcium activated potassium channel messenger RNA nicotine nicotinic receptor nicotinic receptor alpha10 subunit nicotinic receptor alpha9 subunit potassium receptor subunit unclassified drug Alpha10 gene Alpha9 gene animal cell animal tissue article auditory stimulation calcium transport cochlea Corti organ depolarization efferent nerve gene hair cell hearing in situ hybridization ion current knockout gene newborn nonhuman postnatal development priority journal protein expression rat receptor potential sensory nerve signal transduction sk2 gene synapse synaptogenesis Acetylcholine Action Potentials Age Factors Animals Cochlea Gene Expression Regulation, Developmental Hair Cells, Inner Hearing Patch-Clamp Techniques Potassium Potassium Channels, Calcium-Activated Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Small-Conductance Calcium-Activated Potassium Channels Synaptic Transmission Developmental regulation of nicotinic synapses on cochlear inner hair cells info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_02706474_v24_n36_p7814_Katz_oai |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-145 |
collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
topic |
α9α10 nAChR Ca2+-activated K+ channel Cholinergic Efferent innervation IHC Mammalian cochlea Neonatal development Transient synapse acetylcholine calcium calcium activated potassium channel messenger RNA nicotine nicotinic receptor nicotinic receptor alpha10 subunit nicotinic receptor alpha9 subunit potassium receptor subunit unclassified drug Alpha10 gene Alpha9 gene animal cell animal tissue article auditory stimulation calcium transport cochlea Corti organ depolarization efferent nerve gene hair cell hearing in situ hybridization ion current knockout gene newborn nonhuman postnatal development priority journal protein expression rat receptor potential sensory nerve signal transduction sk2 gene synapse synaptogenesis Acetylcholine Action Potentials Age Factors Animals Cochlea Gene Expression Regulation, Developmental Hair Cells, Inner Hearing Patch-Clamp Techniques Potassium Potassium Channels, Calcium-Activated Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Small-Conductance Calcium-Activated Potassium Channels Synaptic Transmission |
spellingShingle |
α9α10 nAChR Ca2+-activated K+ channel Cholinergic Efferent innervation IHC Mammalian cochlea Neonatal development Transient synapse acetylcholine calcium calcium activated potassium channel messenger RNA nicotine nicotinic receptor nicotinic receptor alpha10 subunit nicotinic receptor alpha9 subunit potassium receptor subunit unclassified drug Alpha10 gene Alpha9 gene animal cell animal tissue article auditory stimulation calcium transport cochlea Corti organ depolarization efferent nerve gene hair cell hearing in situ hybridization ion current knockout gene newborn nonhuman postnatal development priority journal protein expression rat receptor potential sensory nerve signal transduction sk2 gene synapse synaptogenesis Acetylcholine Action Potentials Age Factors Animals Cochlea Gene Expression Regulation, Developmental Hair Cells, Inner Hearing Patch-Clamp Techniques Potassium Potassium Channels, Calcium-Activated Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Small-Conductance Calcium-Activated Potassium Channels Synaptic Transmission Katz, E. Elgoyhen, A.B. Gómez-Casati, M.E. Knipper, M. Vetter, D.E. Fuchs, P.A. Glowatzki, E. Developmental regulation of nicotinic synapses on cochlear inner hair cells |
topic_facet |
α9α10 nAChR Ca2+-activated K+ channel Cholinergic Efferent innervation IHC Mammalian cochlea Neonatal development Transient synapse acetylcholine calcium calcium activated potassium channel messenger RNA nicotine nicotinic receptor nicotinic receptor alpha10 subunit nicotinic receptor alpha9 subunit potassium receptor subunit unclassified drug Alpha10 gene Alpha9 gene animal cell animal tissue article auditory stimulation calcium transport cochlea Corti organ depolarization efferent nerve gene hair cell hearing in situ hybridization ion current knockout gene newborn nonhuman postnatal development priority journal protein expression rat receptor potential sensory nerve signal transduction sk2 gene synapse synaptogenesis Acetylcholine Action Potentials Age Factors Animals Cochlea Gene Expression Regulation, Developmental Hair Cells, Inner Hearing Patch-Clamp Techniques Potassium Potassium Channels, Calcium-Activated Protein Subunits Rats Rats, Sprague-Dawley Receptors, Nicotinic Small-Conductance Calcium-Activated Potassium Channels Synaptic Transmission |
description |
In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that may contain both α9 and α10 subunits. Calcium influx through that receptor activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse is thought to disappear after the onset of hearing [after postnatal day 12 (P12)]. We documented this developmental transition using whole-cell recordings from IHCs in apical turns of the rat organ of Corti. Acetylcholine elicited ionic currents in 88-100% of IHCs between P3 and P14, but in only 1 of 11 IHCs at P16-P22. Potassium depolarization of efferent terminals caused IPSCs in 67% of IHCs at P3, in 100% at P7-P9, in 93% at P10-P12, but in only 40% at P13-P14 and in none of the IHCs tested between P16 and P22. Earlier work had shown by in situ hybridization that α9 mRNA is expressed in adult IHCs but that α10 mRNA disappears after the onset of hearing. In the present study, antibodies to α10 and to the associated calcium-dependent (SK2) potassium channel showed a similar developmental loss. The correlated expression of these gene products with functional innervation suggests that Alpha10 and SK2, but not Alpha9, are regulated by synaptic activity. Furthermore, this developmental knock-out of α10, but not α9, supports the hypothesis that functional nicotinic acetylcholine receptors in hair cells are heteromers containing both these subunits. |
format |
Artículo Artículo publishedVersion |
author |
Katz, E. Elgoyhen, A.B. Gómez-Casati, M.E. Knipper, M. Vetter, D.E. Fuchs, P.A. Glowatzki, E. |
author_facet |
Katz, E. Elgoyhen, A.B. Gómez-Casati, M.E. Knipper, M. Vetter, D.E. Fuchs, P.A. Glowatzki, E. |
author_sort |
Katz, E. |
title |
Developmental regulation of nicotinic synapses on cochlear inner hair cells |
title_short |
Developmental regulation of nicotinic synapses on cochlear inner hair cells |
title_full |
Developmental regulation of nicotinic synapses on cochlear inner hair cells |
title_fullStr |
Developmental regulation of nicotinic synapses on cochlear inner hair cells |
title_full_unstemmed |
Developmental regulation of nicotinic synapses on cochlear inner hair cells |
title_sort |
developmental regulation of nicotinic synapses on cochlear inner hair cells |
publishDate |
2004 |
url |
http://hdl.handle.net/20.500.12110/paper_02706474_v24_n36_p7814_Katz http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_02706474_v24_n36_p7814_Katz_oai |
work_keys_str_mv |
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_version_ |
1766026682727137280 |