Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fra...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01455680_v44_n3_p513_Parussini_oai |
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I28-R145-paper_01455680_v44_n3_p513_Parussini_oai2020-10-19 Parussini, F. Duschak, V.G. Cazzulo, J.J. 1998 Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite. Fil:Parussini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Cell. Mol. Biol. (Noisy-le-grand) 1998;44(3):513-519 cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01455680_v44_n3_p513_Parussini_oai |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-145 |
collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
topic |
cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi |
spellingShingle |
cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi Parussini, F. Duschak, V.G. Cazzulo, J.J. Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. |
topic_facet |
cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi |
description |
Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite. |
format |
Artículo Artículo publishedVersion |
author |
Parussini, F. Duschak, V.G. Cazzulo, J.J. |
author_facet |
Parussini, F. Duschak, V.G. Cazzulo, J.J. |
author_sort |
Parussini, F. |
title |
Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. |
title_short |
Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. |
title_full |
Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. |
title_fullStr |
Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. |
title_full_unstemmed |
Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. |
title_sort |
membrane-bound cysteine proteinase isoforms in different developmental stages of trypanosoma cruzi. |
publishDate |
1998 |
url |
http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01455680_v44_n3_p513_Parussini_oai |
work_keys_str_mv |
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1766026666860085248 |