A novel procedure to measure the antioxidant capacity of yerba maté extracts

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Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult t...

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Autores principales:	Hartwig, V.G., Brumovsky, L.A., Fretes, R.M., Boado, L.S.
Formato:	Artículo publishedVersion
Publicado:	2012
Materias:	Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01012061_v32_n1_p126_Hartwig_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_01012061_v32_n1_p126_Hartwig_oai
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spelling	I28-R145-paper_01012061_v32_n1_p126_Hartwig_oai2024-08-16 Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S. 2012 Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 μL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 μM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay. application/pdf http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Cienc. Tecnol. Aliment. 2012;32(1):126-133 Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis A novel procedure to measure the antioxidant capacity of yerba maté extracts info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01012061_v32_n1_p126_Hartwig_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis
spellingShingle	Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S. A novel procedure to measure the antioxidant capacity of yerba maté extracts
topic_facet	Antioxidant capacity Dpph Ilex paraguariensis Yerba maté Croton ovalifolius Ilex paraguariensis
description	Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 μL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 μM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay.
format	Artículo Artículo publishedVersion
author	Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S.
author_facet	Hartwig, V.G. Brumovsky, L.A. Fretes, R.M. Boado, L.S.
author_sort	Hartwig, V.G.
title	A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_short	A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_full	A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_fullStr	A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_full_unstemmed	A novel procedure to measure the antioxidant capacity of yerba maté extracts
title_sort	novel procedure to measure the antioxidant capacity of yerba maté extracts
publishDate	2012
url	http://hdl.handle.net/20.500.12110/paper_01012061_v32_n1_p126_Hartwig https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_01012061_v32_n1_p126_Hartwig_oai
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A novel procedure to measure the antioxidant capacity of yerba maté extracts

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