Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis

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We assessed the effects of different arcA mutations on poly(3- hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some...

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Autores principales:	Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S.
Formato:	Artículo publishedVersion
Publicado:	2006
Materias:	Biomass Cell culture Escherichia coli Mutagenesis Strain Synthesis (chemical) ArcA mutants Leaky mutation Microaerobiosis Poly(3-hydroxybutyrate) synthesis Organic polymers poly(3 hydroxybutyric acid) bacteriology gene mutagenicity recombination aerobic metabolism allele arcA gene arcA2 gene article Azotobacter Azotobacter FA8 bacterial gene bacterial growth bacterial strain biomass bioreactor dry weight gene deletion gene mutation nonhuman pha gene phenotype synthesis wild type Aerobiosis Bacterial Outer Membrane Proteins Culture Media Escherichia coli Proteins Gene Expression Regulation, Bacterial Hydroxybutyrates Mutation Polyesters Recombination, Genetic Repressor Proteins Arca Bacteria (microorganisms)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v72_n4_p2614_Nikel https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v72_n4_p2614_Nikel_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-paper_00992240_v72_n4_p2614_Nikel_oai2024-08-16 Nikel, P.I. Pettinari, M.J. Galvagno, M.A. Méndez, B.S. 2006 We assessed the effects of different arcA mutations on poly(3- hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis was not detected in the wild-type strain in shaken flask cultures under low-oxygen conditions, while ArcA mutants gave rise to polymer accumulation of up to 24% of their cell dry weight. When grown under microaerobic conditions in a bioreactor, the arcA deletion mutant reached a PHB content of 27% ± 2%. Under the same conditions, higher biomass and PHB concentrations were observed for the strain bearing the arcA2 allele, resulting in a PHB content of 35% ± 3%. This strain grew in a simple medium at a specific growth rate of 0.69 ± 0.07 h-1, whereas the deletion mutant needed several nutritional additives and snowed a specific growth rate of 0.56 ± 0.06 h-1. The results presented here suggest that arcA mutations could play a role in heterologous PHB synthesis in microaerobiosis. Copyright © 2006, American Society for Microbiology. All Rights Reserved. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00992240_v72_n4_p2614_Nikel info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Appl. Environ. Microbiol. 2006;72(4):2614-2620 Biomass Cell culture Escherichia coli Mutagenesis Strain Synthesis (chemical) ArcA mutants Leaky mutation Microaerobiosis Poly(3-hydroxybutyrate) synthesis Organic polymers poly(3 hydroxybutyric acid) bacteriology gene mutagenicity recombination aerobic metabolism allele arcA gene arcA2 gene article Azotobacter Azotobacter FA8 bacterial gene bacterial growth bacterial strain biomass bioreactor dry weight Escherichia coli gene deletion gene mutation nonhuman pha gene phenotype synthesis wild type Aerobiosis Bacterial Outer Membrane Proteins Biomass Culture Media Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Hydroxybutyrates Mutation Polyesters Recombination, Genetic Repressor Proteins Arca Azotobacter Bacteria (microorganisms) Escherichia coli Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v72_n4_p2614_Nikel_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Biomass Cell culture Escherichia coli Mutagenesis Strain Synthesis (chemical) ArcA mutants Leaky mutation Microaerobiosis Poly(3-hydroxybutyrate) synthesis Organic polymers poly(3 hydroxybutyric acid) bacteriology gene mutagenicity recombination aerobic metabolism allele arcA gene arcA2 gene article Azotobacter Azotobacter FA8 bacterial gene bacterial growth bacterial strain biomass bioreactor dry weight Escherichia coli gene deletion gene mutation nonhuman pha gene phenotype synthesis wild type Aerobiosis Bacterial Outer Membrane Proteins Biomass Culture Media Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Hydroxybutyrates Mutation Polyesters Recombination, Genetic Repressor Proteins Arca Azotobacter Bacteria (microorganisms) Escherichia coli
spellingShingle	Biomass Cell culture Escherichia coli Mutagenesis Strain Synthesis (chemical) ArcA mutants Leaky mutation Microaerobiosis Poly(3-hydroxybutyrate) synthesis Organic polymers poly(3 hydroxybutyric acid) bacteriology gene mutagenicity recombination aerobic metabolism allele arcA gene arcA2 gene article Azotobacter Azotobacter FA8 bacterial gene bacterial growth bacterial strain biomass bioreactor dry weight Escherichia coli gene deletion gene mutation nonhuman pha gene phenotype synthesis wild type Aerobiosis Bacterial Outer Membrane Proteins Biomass Culture Media Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Hydroxybutyrates Mutation Polyesters Recombination, Genetic Repressor Proteins Arca Azotobacter Bacteria (microorganisms) Escherichia coli Nikel, P.I. Pettinari, M.J. Galvagno, M.A. Méndez, B.S. Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis
topic_facet	Biomass Cell culture Escherichia coli Mutagenesis Strain Synthesis (chemical) ArcA mutants Leaky mutation Microaerobiosis Poly(3-hydroxybutyrate) synthesis Organic polymers poly(3 hydroxybutyric acid) bacteriology gene mutagenicity recombination aerobic metabolism allele arcA gene arcA2 gene article Azotobacter Azotobacter FA8 bacterial gene bacterial growth bacterial strain biomass bioreactor dry weight Escherichia coli gene deletion gene mutation nonhuman pha gene phenotype synthesis wild type Aerobiosis Bacterial Outer Membrane Proteins Biomass Culture Media Escherichia coli Escherichia coli Proteins Gene Expression Regulation, Bacterial Hydroxybutyrates Mutation Polyesters Recombination, Genetic Repressor Proteins Arca Azotobacter Bacteria (microorganisms) Escherichia coli
description	We assessed the effects of different arcA mutations on poly(3- hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis was not detected in the wild-type strain in shaken flask cultures under low-oxygen conditions, while ArcA mutants gave rise to polymer accumulation of up to 24% of their cell dry weight. When grown under microaerobic conditions in a bioreactor, the arcA deletion mutant reached a PHB content of 27% ± 2%. Under the same conditions, higher biomass and PHB concentrations were observed for the strain bearing the arcA2 allele, resulting in a PHB content of 35% ± 3%. This strain grew in a simple medium at a specific growth rate of 0.69 ± 0.07 h-1, whereas the deletion mutant needed several nutritional additives and snowed a specific growth rate of 0.56 ± 0.06 h-1. The results presented here suggest that arcA mutations could play a role in heterologous PHB synthesis in microaerobiosis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
format	Artículo Artículo publishedVersion
author	Nikel, P.I. Pettinari, M.J. Galvagno, M.A. Méndez, B.S.
author_facet	Nikel, P.I. Pettinari, M.J. Galvagno, M.A. Méndez, B.S.
author_sort	Nikel, P.I.
title	Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis
title_short	Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis
title_full	Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis
title_fullStr	Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis
title_full_unstemmed	Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis
title_sort	poly(3-hydroxybutyrate) synthesis by recombinant escherichia coli arca mutants in microaerobiosis
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_00992240_v72_n4_p2614_Nikel https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v72_n4_p2614_Nikel_oai
work_keys_str_mv	AT nikelpi poly3hydroxybutyratesynthesisbyrecombinantescherichiacoliarcamutantsinmicroaerobiosis AT pettinarimj poly3hydroxybutyratesynthesisbyrecombinantescherichiacoliarcamutantsinmicroaerobiosis AT galvagnoma poly3hydroxybutyratesynthesisbyrecombinantescherichiacoliarcamutantsinmicroaerobiosis AT mendezbs poly3hydroxybutyratesynthesisbyrecombinantescherichiacoliarcamutantsinmicroaerobiosis
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Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis

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