ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis

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In organello starch biosynthesis was studied using intact chloroplasts isolated from spinach leaves (Spinacia oleracea). Immunoblot analysis using a specific antiserum against the mitochondrial adenylate (ADP/ATP) translocator of Neurospora crassa shows the presence of an adenylate translocator prot...

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Autores principales: Pozueta-Romero, J., Ardila, F., Akazawa, T.
Formato: Artículo publishedVersion
Publicado: 1991
Materias:
Acer
Acer pseudoplatanus
Neurospora crassa
Spinacia oleracea
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00320889_v97_n4_p1565_PozuetaRomero
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00320889_v97_n4_p1565_PozuetaRomero_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00320889_v97_n4_p1565_PozuetaRomero_oai
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spelling I28-R145-paper_00320889_v97_n4_p1565_PozuetaRomero_oai2024-08-16 Pozueta-Romero, J. Ardila, F. Akazawa, T. 1991 In organello starch biosynthesis was studied using intact chloroplasts isolated from spinach leaves (Spinacia oleracea). Immunoblot analysis using a specific antiserum against the mitochondrial adenylate (ADP/ATP) translocator of Neurospora crassa shows the presence of an adenylate translocator protein in the chloroplast envelope membranes, similar to that existing in mitochondria and amyloplasts from cultured cells of sycamore (Acer pseudoplatanus). The double silicone oil layer-filtering centrifugation technique was employed to study the kinetic properties of adenylate transport in the purified chloroplasts; ATP, ADP, AMP, and most importantly ADP-Glc were shown to be recognized by the adenylate translocator. Similar to the situation with sycamore amyloplasts, only ATP and ADP-Glc uptake was inhibited by carboxyatractyloside, an inhibitor of the mitochondrial adenylate translocator. Evidence is presented to show that the ADP-Glc transported into the chloroplast stroma is utilized for starch synthesis catalyzed by starch synthase (ADP-Glc: 1,4-α-d-glucan 4-α-d-glucosyltransferase). The high activity of sucrose synthase producing ADP-Glc observed in the extrachloroplastic fractions suggests that starch biosynthesis in chloroplasts may be coupled with the direct import of ADP-Glc from the cytosol. Fil:Ardila, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00320889_v97_n4_p1565_PozuetaRomero info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Plant Physiol. 1991;97(4):1565-1572 Acer Acer pseudoplatanus Neurospora crassa Spinacia oleracea ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00320889_v97_n4_p1565_PozuetaRomero_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic Acer
Acer pseudoplatanus
Neurospora crassa
Spinacia oleracea
spellingShingle Acer
Acer pseudoplatanus
Neurospora crassa
Spinacia oleracea
Pozueta-Romero, J.
Ardila, F.
Akazawa, T.
ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
topic_facet Acer
Acer pseudoplatanus
Neurospora crassa
Spinacia oleracea
description In organello starch biosynthesis was studied using intact chloroplasts isolated from spinach leaves (Spinacia oleracea). Immunoblot analysis using a specific antiserum against the mitochondrial adenylate (ADP/ATP) translocator of Neurospora crassa shows the presence of an adenylate translocator protein in the chloroplast envelope membranes, similar to that existing in mitochondria and amyloplasts from cultured cells of sycamore (Acer pseudoplatanus). The double silicone oil layer-filtering centrifugation technique was employed to study the kinetic properties of adenylate transport in the purified chloroplasts; ATP, ADP, AMP, and most importantly ADP-Glc were shown to be recognized by the adenylate translocator. Similar to the situation with sycamore amyloplasts, only ATP and ADP-Glc uptake was inhibited by carboxyatractyloside, an inhibitor of the mitochondrial adenylate translocator. Evidence is presented to show that the ADP-Glc transported into the chloroplast stroma is utilized for starch synthesis catalyzed by starch synthase (ADP-Glc: 1,4-α-d-glucan 4-α-d-glucosyltransferase). The high activity of sucrose synthase producing ADP-Glc observed in the extrachloroplastic fractions suggests that starch biosynthesis in chloroplasts may be coupled with the direct import of ADP-Glc from the cytosol.
format Artículo
Artículo
publishedVersion
author Pozueta-Romero, J.
Ardila, F.
Akazawa, T.
author_facet Pozueta-Romero, J.
Ardila, F.
Akazawa, T.
author_sort Pozueta-Romero, J.
title ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
title_short ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
title_full ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
title_fullStr ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
title_full_unstemmed ADP-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
title_sort adp-glucose transport by the chloroplast adenylate translocator is linked to starch biosynthesis
publishDate 1991
url http://hdl.handle.net/20.500.12110/paper_00320889_v97_n4_p1565_PozuetaRomero
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00320889_v97_n4_p1565_PozuetaRomero_oai
work_keys_str_mv AT pozuetaromeroj adpglucosetransportbythechloroplastadenylatetranslocatorislinkedtostarchbiosynthesis
AT ardilaf adpglucosetransportbythechloroplastadenylatetranslocatorislinkedtostarchbiosynthesis
AT akazawat adpglucosetransportbythechloroplastadenylatetranslocatorislinkedtostarchbiosynthesis
_version_ 1809356798741708800

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