Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana susp...
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2005
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00320781_v46_n9_p1494_Zhang_oai |
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I28-R145-paper_00320781_v46_n9_p1494_Zhang_oai2024-08-16 Zhang, Z. Ramirez, J. Reboutier, D. Brault, M. Trouverie, J. Pennarun, A.-M. Amiar, Z. Biligui, B. Galagovsky, L. Rona, J.-P. 2005 Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana suspension cells. We also show that cell expansions induced by HBL and HCS are correlated with the amplitude of the plasma membrane hyperpolarization they elicited. HBL, which promoted the larger cell expansion, also provoked the larger hyperpolarization. We observed that membrane hyperpolarization and cell expansion were partially inhibited by the proton pump inhibitor erythrosin B, suggesting that proton pumps were not the only ion transport system modulated by the two BRs. We used a voltage clamp approach in order to find the other ion transport systems involved in the PM hyperpolarization elicited by HBL and HCS. Interestingly, while anion currents were inhibited by both HBL and HCS, outward rectifying K+ currents were increased by HBL but inhibited by HCS. The different electrophysiological behavior shown by HBL and HCS indicates that small changes in the BR skeleton might be responsible for changes in bioactivity. © 2005 JSPP. Fil:Ramirez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Plant Cell Physiol. 2005;46(9):1494-1504 Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00320781_v46_n9_p1494_Zhang_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana |
| spellingShingle |
Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana Zhang, Z. Ramirez, J. Reboutier, D. Brault, M. Trouverie, J. Pennarun, A.-M. Amiar, Z. Biligui, B. Galagovsky, L. Rona, J.-P. Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells |
| topic_facet |
Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana |
| description |
Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana suspension cells. We also show that cell expansions induced by HBL and HCS are correlated with the amplitude of the plasma membrane hyperpolarization they elicited. HBL, which promoted the larger cell expansion, also provoked the larger hyperpolarization. We observed that membrane hyperpolarization and cell expansion were partially inhibited by the proton pump inhibitor erythrosin B, suggesting that proton pumps were not the only ion transport system modulated by the two BRs. We used a voltage clamp approach in order to find the other ion transport systems involved in the PM hyperpolarization elicited by HBL and HCS. Interestingly, while anion currents were inhibited by both HBL and HCS, outward rectifying K+ currents were increased by HBL but inhibited by HCS. The different electrophysiological behavior shown by HBL and HCS indicates that small changes in the BR skeleton might be responsible for changes in bioactivity. © 2005 JSPP. |
| format |
Artículo Artículo publishedVersion |
| author |
Zhang, Z. Ramirez, J. Reboutier, D. Brault, M. Trouverie, J. Pennarun, A.-M. Amiar, Z. Biligui, B. Galagovsky, L. Rona, J.-P. |
| author_facet |
Zhang, Z. Ramirez, J. Reboutier, D. Brault, M. Trouverie, J. Pennarun, A.-M. Amiar, Z. Biligui, B. Galagovsky, L. Rona, J.-P. |
| author_sort |
Zhang, Z. |
| title |
Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells |
| title_short |
Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells |
| title_full |
Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells |
| title_fullStr |
Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells |
| title_full_unstemmed |
Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells |
| title_sort |
brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of arabidopsis thaliana cells |
| publishDate |
2005 |
| url |
http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00320781_v46_n9_p1494_Zhang_oai |
| work_keys_str_mv |
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