ASR1, a stress-induced tomato protein, protects yeast from osmotic stress

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Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expressi...

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Autores principales: Moretti, M.B., Maskin, L., Gudesblat, G., García, S.C., Iusem, N.D.
Formato: Artículo publishedVersion
Publicado: 2006
Materias:
Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00319317_v127_n1_p111_Moretti_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00319317_v127_n1_p111_Moretti_oai
record_format dspace
spelling I28-R145-paper_00319317_v127_n1_p111_Moretti_oai2024-08-16 Moretti, M.B. Maskin, L. Gudesblat, G. García, S.C. Iusem, N.D. 2006 Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expression system Saccharomyces cerevisiae under the control of a galactose-inducible promoter. In a mutant yeast strain deficient in one component of the stress-responsive high-osmolarity glycerol (HOG) pathway, namely the MAP kinase Hog1, the synthesis of ASR1 protein restores growth under osmotic stress conditions such as 0.5 M NaCl and 1.2 M sorbitol. In contrast, the rescuing of this phenotype was less evident using a wild-type strain or the upstream MAP kinase kinase (Pbs2)-deficient strain. In both knock-out strains impaired in glycerol synthesis because of a dysfunctional HOG pathway, but not in wild-type, ASR1 led to the accumulation of endogenous glycerol in an osmotic stress-independent and unrestrained manner. These data suggest that ASR1 complements yeast HOG-deficient phenotypes by inducing downstream components of the HOG pathway. The results are discussed in terms of the function of ASR proteins in planta at the molecular and cellular level. Copyright © Physiologia Plantarum 2006. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gudesblat, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Physiol. Plant. 2006;127(1):111-118 Biosynthesis Fruits Genes Glycerol Osmosis Yeast Endogenous glycerol Osmotic stress Tomato protein Proteins Tomatoes Lycopersicon esculentum Saccharomyces cerevisiae ASR1, a stress-induced tomato protein, protects yeast from osmotic stress info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00319317_v127_n1_p111_Moretti_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
spellingShingle Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
Moretti, M.B.
Maskin, L.
Gudesblat, G.
García, S.C.
Iusem, N.D.
ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
topic_facet Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
description Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expression system Saccharomyces cerevisiae under the control of a galactose-inducible promoter. In a mutant yeast strain deficient in one component of the stress-responsive high-osmolarity glycerol (HOG) pathway, namely the MAP kinase Hog1, the synthesis of ASR1 protein restores growth under osmotic stress conditions such as 0.5 M NaCl and 1.2 M sorbitol. In contrast, the rescuing of this phenotype was less evident using a wild-type strain or the upstream MAP kinase kinase (Pbs2)-deficient strain. In both knock-out strains impaired in glycerol synthesis because of a dysfunctional HOG pathway, but not in wild-type, ASR1 led to the accumulation of endogenous glycerol in an osmotic stress-independent and unrestrained manner. These data suggest that ASR1 complements yeast HOG-deficient phenotypes by inducing downstream components of the HOG pathway. The results are discussed in terms of the function of ASR proteins in planta at the molecular and cellular level. Copyright © Physiologia Plantarum 2006.
format Artículo
Artículo
publishedVersion
author Moretti, M.B.
Maskin, L.
Gudesblat, G.
García, S.C.
Iusem, N.D.
author_facet Moretti, M.B.
Maskin, L.
Gudesblat, G.
García, S.C.
Iusem, N.D.
author_sort Moretti, M.B.
title ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_short ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_full ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_fullStr ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_full_unstemmed ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_sort asr1, a stress-induced tomato protein, protects yeast from osmotic stress
publishDate 2006
url http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00319317_v127_n1_p111_Moretti_oai
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