Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information

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We investigated the taxonomic status of two sympatric morphospecies of squat lobsters from southern South America (Beagle Channel, Strait of Magellan, and Burdwood Bank), Munida gregaria and Munida subrugosa, by DNA sequence analysis of three mitochondrial (mt)DNA gene fragments [416 bp of 1...

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Autores principales: Pérez-Barros, P., D'Amato, M.E., Guzmán, N.V., Lovrich, G.A.
Formato: Artículo publishedVersion
Publicado: 2008
Materias:
16S
COI
Genetic variation
ITS1
ND1
Speciation
DNA
genetic analysis
genetic variation
lobster
mitochondrial DNA
morphology
speciation (biology)
sympatry
taxonomy
Atlantic Ocean
Beagle Channel
Burdwood Bank
Magellan Strait
South America
Crustacea
Decapoda (Crustacea)
Galatheidae
Munida gregaria
Munida subrugosa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00244066_v94_n2_p421_PerezBarros
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00244066_v94_n2_p421_PerezBarros_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00244066_v94_n2_p421_PerezBarros_oai
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spelling I28-R145-paper_00244066_v94_n2_p421_PerezBarros_oai2024-08-16 Pérez-Barros, P. D'Amato, M.E. Guzmán, N.V. Lovrich, G.A. 2008 We investigated the taxonomic status of two sympatric morphospecies of squat lobsters from southern South America (Beagle Channel, Strait of Magellan, and Burdwood Bank), Munida gregaria and Munida subrugosa, by DNA sequence analysis of three mitochondrial (mt)DNA gene fragments [416 bp of 16S rDNA(165), 566 bp of cytochrome c oxidase subunit I(COI) and 418 bp of NADH dehydrogenase subunit 1 (ND1)]; and the nuclear rDNA internal transcribed spacer (ITS) 1 (883-952 bp). We obtained a total of 79 sequences from 32 individuals. The 16S sequences of all M. gregaria and M. subrugosa were invariant and identical, whereas COI and ND1 showed 12 and 15 variable sites, respectively. These polymorphisms were shared between morphospecies. Interspecific Tamura-Nei distances for COI and ND1 sequences were 0.0024 and 0.0032, respectively, and were not significantly different from intraspecific distances (Kruskal-Wallis tests: P = 0.58 and P = 0.69, for COI and ND1, respectively). Similar to the results obtained from the mtDNA sequences, no relationship was found between the ITS1 maximum parsimony tree topology and the morphologic classification of specimens in M. gregaria and M. subrugosa. We conclude that M. gregaria and M. subrugosa from southern South America may either represent a case of a dimorphic species, or a case of incomplete lineage sorting. The fact that these two morphospecies did not show fixed differences over a total of 1947 bp analysed reinforces the hypothesis of a single dimorphic species. © 2008 The Linnean Society of London. Fil:Pérez-Barros, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guzmán, N.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lovrich, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00244066_v94_n2_p421_PerezBarros info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Biol. J. Linn. Soc. 2008;94(2):421-434 16S COI Genetic variation ITS1 ND1 Speciation DNA genetic analysis genetic variation lobster mitochondrial DNA morphology speciation (biology) sympatry taxonomy Atlantic Ocean Beagle Channel Burdwood Bank Magellan Strait South America Crustacea Decapoda (Crustacea) Galatheidae Munida gregaria Munida subrugosa Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00244066_v94_n2_p421_PerezBarros_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic 16S
COI
Genetic variation
ITS1
ND1
Speciation
DNA
genetic analysis
genetic variation
lobster
mitochondrial DNA
morphology
speciation (biology)
sympatry
taxonomy
Atlantic Ocean
Beagle Channel
Burdwood Bank
Magellan Strait
South America
Crustacea
Decapoda (Crustacea)
Galatheidae
Munida gregaria
Munida subrugosa
spellingShingle 16S
COI
Genetic variation
ITS1
ND1
Speciation
DNA
genetic analysis
genetic variation
lobster
mitochondrial DNA
morphology
speciation (biology)
sympatry
taxonomy
Atlantic Ocean
Beagle Channel
Burdwood Bank
Magellan Strait
South America
Crustacea
Decapoda (Crustacea)
Galatheidae
Munida gregaria
Munida subrugosa
Pérez-Barros, P.
D'Amato, M.E.
Guzmán, N.V.
Lovrich, G.A.
Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information
topic_facet 16S
COI
Genetic variation
ITS1
ND1
Speciation
DNA
genetic analysis
genetic variation
lobster
mitochondrial DNA
morphology
speciation (biology)
sympatry
taxonomy
Atlantic Ocean
Beagle Channel
Burdwood Bank
Magellan Strait
South America
Crustacea
Decapoda (Crustacea)
Galatheidae
Munida gregaria
Munida subrugosa
description We investigated the taxonomic status of two sympatric morphospecies of squat lobsters from southern South America (Beagle Channel, Strait of Magellan, and Burdwood Bank), Munida gregaria and Munida subrugosa, by DNA sequence analysis of three mitochondrial (mt)DNA gene fragments [416 bp of 16S rDNA(165), 566 bp of cytochrome c oxidase subunit I(COI) and 418 bp of NADH dehydrogenase subunit 1 (ND1)]; and the nuclear rDNA internal transcribed spacer (ITS) 1 (883-952 bp). We obtained a total of 79 sequences from 32 individuals. The 16S sequences of all M. gregaria and M. subrugosa were invariant and identical, whereas COI and ND1 showed 12 and 15 variable sites, respectively. These polymorphisms were shared between morphospecies. Interspecific Tamura-Nei distances for COI and ND1 sequences were 0.0024 and 0.0032, respectively, and were not significantly different from intraspecific distances (Kruskal-Wallis tests: P = 0.58 and P = 0.69, for COI and ND1, respectively). Similar to the results obtained from the mtDNA sequences, no relationship was found between the ITS1 maximum parsimony tree topology and the morphologic classification of specimens in M. gregaria and M. subrugosa. We conclude that M. gregaria and M. subrugosa from southern South America may either represent a case of a dimorphic species, or a case of incomplete lineage sorting. The fact that these two morphospecies did not show fixed differences over a total of 1947 bp analysed reinforces the hypothesis of a single dimorphic species. © 2008 The Linnean Society of London.
format Artículo
Artículo
publishedVersion
author Pérez-Barros, P.
D'Amato, M.E.
Guzmán, N.V.
Lovrich, G.A.
author_facet Pérez-Barros, P.
D'Amato, M.E.
Guzmán, N.V.
Lovrich, G.A.
author_sort Pérez-Barros, P.
title Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information
title_short Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information
title_full Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information
title_fullStr Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information
title_full_unstemmed Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information
title_sort taxonomic status of two south american sympatric squat lobsters, munida gregaria and munida subrugosa (crustacea: decapoda: galatheidae), challenged by dna sequence information
publishDate 2008
url http://hdl.handle.net/20.500.12110/paper_00244066_v94_n2_p421_PerezBarros
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00244066_v94_n2_p421_PerezBarros_oai
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AT damatome taxonomicstatusoftwosouthamericansympatricsquatlobstersmunidagregariaandmunidasubrugosacrustaceadecapodagalatheidaechallengedbydnasequenceinformation
AT guzmannv taxonomicstatusoftwosouthamericansympatricsquatlobstersmunidagregariaandmunidasubrugosacrustaceadecapodagalatheidaechallengedbydnasequenceinformation
AT lovrichga taxonomicstatusoftwosouthamericansympatricsquatlobstersmunidagregariaandmunidasubrugosacrustaceadecapodagalatheidaechallengedbydnasequenceinformation
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