Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster...
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2012
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00220949_v215_n13_p2220_Arias http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220949_v215_n13_p2220_Arias_oai |
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I28-R145-paper_00220949_v215_n13_p2220_Arias_oai2020-10-19 Arias, L.N. Sambucetti, P. Scannapieco, A.C. Loeschcke, V. Norry, F.M. 2012 Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E-16F6; 30A3-34C2; 49C-50C). Previously found thermotolerance QTL were also significant for flies from HD cultures. © 2012. Published by The Company of Biologists Ltd. Fil:Arias, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sambucetti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scannapieco, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00220949_v215_n13_p2220_Arias info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Exp. Biol. 2012;215(13):2220-2225 High temperature stress Quantitative trait loci Thermal resistance Thermotolerance adaptation animal article Drosophila melanogaster genetics heat larva phenotype physiological stress physiology quantitative trait locus Adaptation, Physiological Animals Drosophila melanogaster Hot Temperature Larva Phenotype Quantitative Trait Loci Stress, Physiological Drosophila melanogaster Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220949_v215_n13_p2220_Arias_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
High temperature stress Quantitative trait loci Thermal resistance Thermotolerance adaptation animal article Drosophila melanogaster genetics heat larva phenotype physiological stress physiology quantitative trait locus Adaptation, Physiological Animals Drosophila melanogaster Hot Temperature Larva Phenotype Quantitative Trait Loci Stress, Physiological Drosophila melanogaster |
| spellingShingle |
High temperature stress Quantitative trait loci Thermal resistance Thermotolerance adaptation animal article Drosophila melanogaster genetics heat larva phenotype physiological stress physiology quantitative trait locus Adaptation, Physiological Animals Drosophila melanogaster Hot Temperature Larva Phenotype Quantitative Trait Loci Stress, Physiological Drosophila melanogaster Arias, L.N. Sambucetti, P. Scannapieco, A.C. Loeschcke, V. Norry, F.M. Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density |
| topic_facet |
High temperature stress Quantitative trait loci Thermal resistance Thermotolerance adaptation animal article Drosophila melanogaster genetics heat larva phenotype physiological stress physiology quantitative trait locus Adaptation, Physiological Animals Drosophila melanogaster Hot Temperature Larva Phenotype Quantitative Trait Loci Stress, Physiological Drosophila melanogaster |
| description |
Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E-16F6; 30A3-34C2; 49C-50C). Previously found thermotolerance QTL were also significant for flies from HD cultures. © 2012. Published by The Company of Biologists Ltd. |
| format |
Artículo Artículo publishedVersion |
| author |
Arias, L.N. Sambucetti, P. Scannapieco, A.C. Loeschcke, V. Norry, F.M. |
| author_facet |
Arias, L.N. Sambucetti, P. Scannapieco, A.C. Loeschcke, V. Norry, F.M. |
| author_sort |
Arias, L.N. |
| title |
Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density |
| title_short |
Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density |
| title_full |
Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density |
| title_fullStr |
Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density |
| title_full_unstemmed |
Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density |
| title_sort |
survival of heat stress with and without heat hardening in drosophila melanogaster:interactions with larval density |
| publishDate |
2012 |
| url |
http://hdl.handle.net/20.500.12110/paper_00220949_v215_n13_p2220_Arias http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00220949_v215_n13_p2220_Arias_oai |
| work_keys_str_mv |
AT ariasln survivalofheatstresswithandwithoutheathardeningindrosophilamelanogasterinteractionswithlarvaldensity AT sambucettip survivalofheatstresswithandwithoutheathardeningindrosophilamelanogasterinteractionswithlarvaldensity AT scannapiecoac survivalofheatstresswithandwithoutheathardeningindrosophilamelanogasterinteractionswithlarvaldensity AT loeschckev survivalofheatstresswithandwithoutheathardeningindrosophilamelanogasterinteractionswithlarvaldensity AT norryfm survivalofheatstresswithandwithoutheathardeningindrosophilamelanogasterinteractionswithlarvaldensity |
| _version_ |
1766026582487465984 |