Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi
The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epim...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo publishedVersion |
| Publicado: |
1993
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00142956_v218_n3_p929_DeLederkremer https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00142956_v218_n3_p929_DeLederkremer_oai |
| Aporte de: |
| id |
I28-R145-paper_00142956_v218_n3_p929_DeLederkremer_oai |
|---|---|
| record_format |
dspace |
| spelling |
I28-R145-paper_00142956_v218_n3_p929_DeLederkremer_oai2024-08-16 De Lederkremer, R.M. Lima, C.E. Ramirez, M.I. Goncalvez, M.F. Colli, W. 1993 The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epimastigotes after the second day of culture growth. [3H]Palmitic acid was incorporated into 1‐O‐hexadecyl‐2‐O‐palmitoylglycerol in glycoinositolphospholipid A and into ceramide in glycoinositolphospholipid B. The lipids were released by incubation with glycosylphosphatidylinositol‐specific phospholipase C from Bacillus thuringiensis or by chemical methods. After alkaline hydrolysis, the lipids were analysed by GLC/MS. In glycoinositolphospholipid A the resulting lipids corresponded to 1‐O‐hexadecylglycerol and palmitic acid. The ceramide components in glycoinositolphospholipid B are sphinganine, palmitic acid and lignoceric acid. The oligosaccharides could be degraded by nitrous acid and further enzymic treatment showed that the two glycoinositolphospholipids isolated from T. cruzi share the common core structure of the glycosylphosphatidylinositol membrane anchors. The microheterogeneity was determined, as well as the substitution by galactose, and was mainly in the furanose configuration as was previously described for lipopeptidophosphoglycan. However, methylation analysis indicated that 20% of the galactose is in the pyranose from. Both glycoinositolphospholipids mainly differ in the lipid moiety. Copyright © 1993, Wiley Blackwell. All rights reserved Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramirez, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00142956_v218_n3_p929_DeLederkremer info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Eur. J. Biochem. 1993;218(3):929-936 ceramide glycoinositolphospholipid glycophospoinositol phospholipase c unclassified drug article epimastigote nonhuman priority journal trypanosoma cruzi Animal Carbohydrate Sequence Ceramides Chromatography, Gas Glyceryl Ethers Glycosylation Glycosylphosphatidylinositols Lipids Methylation Molecular Sequence Data Palmitates Polysaccharides Spectrum Analysis, Mass Support, Non-U.S. Gov't Trypanosoma cruzi Bacillus thuringiensis Trypanosoma Trypanosoma cruzi Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00142956_v218_n3_p929_DeLederkremer_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
ceramide glycoinositolphospholipid glycophospoinositol phospholipase c unclassified drug article epimastigote nonhuman priority journal trypanosoma cruzi Animal Carbohydrate Sequence Ceramides Chromatography, Gas Glyceryl Ethers Glycosylation Glycosylphosphatidylinositols Lipids Methylation Molecular Sequence Data Palmitates Polysaccharides Spectrum Analysis, Mass Support, Non-U.S. Gov't Trypanosoma cruzi Bacillus thuringiensis Trypanosoma Trypanosoma cruzi |
| spellingShingle |
ceramide glycoinositolphospholipid glycophospoinositol phospholipase c unclassified drug article epimastigote nonhuman priority journal trypanosoma cruzi Animal Carbohydrate Sequence Ceramides Chromatography, Gas Glyceryl Ethers Glycosylation Glycosylphosphatidylinositols Lipids Methylation Molecular Sequence Data Palmitates Polysaccharides Spectrum Analysis, Mass Support, Non-U.S. Gov't Trypanosoma cruzi Bacillus thuringiensis Trypanosoma Trypanosoma cruzi De Lederkremer, R.M. Lima, C.E. Ramirez, M.I. Goncalvez, M.F. Colli, W. Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi |
| topic_facet |
ceramide glycoinositolphospholipid glycophospoinositol phospholipase c unclassified drug article epimastigote nonhuman priority journal trypanosoma cruzi Animal Carbohydrate Sequence Ceramides Chromatography, Gas Glyceryl Ethers Glycosylation Glycosylphosphatidylinositols Lipids Methylation Molecular Sequence Data Palmitates Polysaccharides Spectrum Analysis, Mass Support, Non-U.S. Gov't Trypanosoma cruzi Bacillus thuringiensis Trypanosoma Trypanosoma cruzi |
| description |
The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epimastigotes after the second day of culture growth. [3H]Palmitic acid was incorporated into 1‐O‐hexadecyl‐2‐O‐palmitoylglycerol in glycoinositolphospholipid A and into ceramide in glycoinositolphospholipid B. The lipids were released by incubation with glycosylphosphatidylinositol‐specific phospholipase C from Bacillus thuringiensis or by chemical methods. After alkaline hydrolysis, the lipids were analysed by GLC/MS. In glycoinositolphospholipid A the resulting lipids corresponded to 1‐O‐hexadecylglycerol and palmitic acid. The ceramide components in glycoinositolphospholipid B are sphinganine, palmitic acid and lignoceric acid. The oligosaccharides could be degraded by nitrous acid and further enzymic treatment showed that the two glycoinositolphospholipids isolated from T. cruzi share the common core structure of the glycosylphosphatidylinositol membrane anchors. The microheterogeneity was determined, as well as the substitution by galactose, and was mainly in the furanose configuration as was previously described for lipopeptidophosphoglycan. However, methylation analysis indicated that 20% of the galactose is in the pyranose from. Both glycoinositolphospholipids mainly differ in the lipid moiety. Copyright © 1993, Wiley Blackwell. All rights reserved |
| format |
Artículo Artículo publishedVersion |
| author |
De Lederkremer, R.M. Lima, C.E. Ramirez, M.I. Goncalvez, M.F. Colli, W. |
| author_facet |
De Lederkremer, R.M. Lima, C.E. Ramirez, M.I. Goncalvez, M.F. Colli, W. |
| author_sort |
De Lederkremer, R.M. |
| title |
Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi |
| title_short |
Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi |
| title_full |
Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi |
| title_fullStr |
Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi |
| title_full_unstemmed |
Hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in Trypanosoma cruzi |
| title_sort |
hexadecylpalmitoyglycerol or ceramide is linked to similar glycophosphoinositol anchor‐like structures in trypanosoma cruzi |
| publishDate |
1993 |
| url |
http://hdl.handle.net/20.500.12110/paper_00142956_v218_n3_p929_DeLederkremer https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00142956_v218_n3_p929_DeLederkremer_oai |
| work_keys_str_mv |
AT delederkremerrm hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi AT limace hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi AT ramirezmi hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi AT goncalvezmf hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi AT colliw hexadecylpalmitoyglycerolorceramideislinkedtosimilarglycophosphoinositolanchorlikestructuresintrypanosomacruzi |
| _version_ |
1809357191194345472 |