Aldosterone biosynthesis in the rat brain

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Messenger RNA (mRNA) for enzymes involved in adrenal steroid biosynthesis are expressed in the brain, and the coded enzymes have been shown to be active. The expression of mRNA for the cytochrome P-450 enzyme aldosterone synthase, crucial for the final step in the synthesis of aldosterone and the sy...

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Autores principales:	Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Foecking, M.F., Gomez-Sanchez, E.P.
Formato:	Artículo publishedVersion
Publicado:	1997
Materias:	18 hydroxydeoxycorticosterone aldosterone corticosterone messenger RNA synthetase amygdaloid nucleus animal tissue article brain cerebellum controlled study gene expression hippocampus hormone synthesis hypothalamus male nonhuman priority journal rat reverse transcription polymerase chain reaction Southern blotting steroidogenesis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00137227_v138_n8_p3369_GomezSanchez http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00137227_v138_n8_p3369_GomezSanchez_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_00137227_v138_n8_p3369_GomezSanchez_oai
record_format	dspace
spelling	I28-R145-paper_00137227_v138_n8_p3369_GomezSanchez_oai2020-10-19 Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Foecking, M.F. Gomez-Sanchez, E.P. 1997 Messenger RNA (mRNA) for enzymes involved in adrenal steroid biosynthesis are expressed in the brain, and the coded enzymes have been shown to be active. The expression of mRNA for the cytochrome P-450 enzyme aldosterone synthase, crucial for the final step in the synthesis of aldosterone and the synthesis of aldosterone was studied in several anatomic areas of the rat brain. Expression of the mRNA for the aldosterone synthase was demonstrated by RT-PCR/Southern blot in adrenal, aorta, hypothalamus, hippocampus, amygdala, cerebrum, and cerebellum. Incubation of brain minces from intact and adrenalectomized rats demonstrated the synthesis of corticosterone and aldosterone from endogenous precursors. Incubations of brain mince, with [1,23H]-deoxycorticosterone, followed by extraction and three different successive TLCs, demonstrated the presence of labeled aldosterone, corticosterone, and 18-hydroxy-deoxycorticosterone. Incubation, in the presence of 10 μM cortisol or metyrapone, inhibited the synthesis of aldosterone or both aldosterone and corticosterone, respectively. These studies indicate that the rat brain has the enzymatic machinery for the synthesis of adrenal corticosteroids and is capable of synthesizing aldosterone. Aldosterone synthesized in the brain might play a paracrine role in the regulation of blood pressure. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00137227_v138_n8_p3369_GomezSanchez info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar ENDOCRINOLOGY 1997;138(8):3369-3373 18 hydroxydeoxycorticosterone aldosterone corticosterone messenger RNA synthetase amygdaloid nucleus animal tissue article brain cerebellum controlled study gene expression hippocampus hormone synthesis hypothalamus male nonhuman priority journal rat reverse transcription polymerase chain reaction Southern blotting steroidogenesis Aldosterone biosynthesis in the rat brain info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00137227_v138_n8_p3369_GomezSanchez_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	18 hydroxydeoxycorticosterone aldosterone corticosterone messenger RNA synthetase amygdaloid nucleus animal tissue article brain cerebellum controlled study gene expression hippocampus hormone synthesis hypothalamus male nonhuman priority journal rat reverse transcription polymerase chain reaction Southern blotting steroidogenesis
spellingShingle	18 hydroxydeoxycorticosterone aldosterone corticosterone messenger RNA synthetase amygdaloid nucleus animal tissue article brain cerebellum controlled study gene expression hippocampus hormone synthesis hypothalamus male nonhuman priority journal rat reverse transcription polymerase chain reaction Southern blotting steroidogenesis Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Foecking, M.F. Gomez-Sanchez, E.P. Aldosterone biosynthesis in the rat brain
topic_facet	18 hydroxydeoxycorticosterone aldosterone corticosterone messenger RNA synthetase amygdaloid nucleus animal tissue article brain cerebellum controlled study gene expression hippocampus hormone synthesis hypothalamus male nonhuman priority journal rat reverse transcription polymerase chain reaction Southern blotting steroidogenesis
description	Messenger RNA (mRNA) for enzymes involved in adrenal steroid biosynthesis are expressed in the brain, and the coded enzymes have been shown to be active. The expression of mRNA for the cytochrome P-450 enzyme aldosterone synthase, crucial for the final step in the synthesis of aldosterone and the synthesis of aldosterone was studied in several anatomic areas of the rat brain. Expression of the mRNA for the aldosterone synthase was demonstrated by RT-PCR/Southern blot in adrenal, aorta, hypothalamus, hippocampus, amygdala, cerebrum, and cerebellum. Incubation of brain minces from intact and adrenalectomized rats demonstrated the synthesis of corticosterone and aldosterone from endogenous precursors. Incubations of brain mince, with [1,23H]-deoxycorticosterone, followed by extraction and three different successive TLCs, demonstrated the presence of labeled aldosterone, corticosterone, and 18-hydroxy-deoxycorticosterone. Incubation, in the presence of 10 μM cortisol or metyrapone, inhibited the synthesis of aldosterone or both aldosterone and corticosterone, respectively. These studies indicate that the rat brain has the enzymatic machinery for the synthesis of adrenal corticosteroids and is capable of synthesizing aldosterone. Aldosterone synthesized in the brain might play a paracrine role in the regulation of blood pressure.
format	Artículo Artículo publishedVersion
author	Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Foecking, M.F. Gomez-Sanchez, E.P.
author_facet	Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Foecking, M.F. Gomez-Sanchez, E.P.
author_sort	Gomez-Sanchez, C.E.
title	Aldosterone biosynthesis in the rat brain
title_short	Aldosterone biosynthesis in the rat brain
title_full	Aldosterone biosynthesis in the rat brain
title_fullStr	Aldosterone biosynthesis in the rat brain
title_full_unstemmed	Aldosterone biosynthesis in the rat brain
title_sort	aldosterone biosynthesis in the rat brain
publishDate	1997
url	http://hdl.handle.net/20.500.12110/paper_00137227_v138_n8_p3369_GomezSanchez http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00137227_v138_n8_p3369_GomezSanchez_oai
work_keys_str_mv	AT gomezsanchezce aldosteronebiosynthesisintheratbrain AT zhoumy aldosteronebiosynthesisintheratbrain AT cozzaen aldosteronebiosynthesisintheratbrain AT moritah aldosteronebiosynthesisintheratbrain AT foeckingmf aldosteronebiosynthesisintheratbrain AT gomezsanchezep aldosteronebiosynthesisintheratbrain
_version_	1766026521047203840

Aldosterone biosynthesis in the rat brain

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