Homeostasis de ATP extracelular en eritrocitos: interregulación y consecuencias funcionales
Human erythrocytes (RBCs) release ATP in different stimuli.\nIn vitro, exposure to different pharmacological agents triggers intracellular\nevents that activate ATP release.\nIn this thesis factors controlling the temporal evolution of extracellular ATP\n(ATPe) concentration were analyzed. Cells wer...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2015
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_819 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_819.dir/819.PDF |
| Aporte de: |
| Sumario: | Human erythrocytes (RBCs) release ATP in different stimuli.\nIn vitro, exposure to different pharmacological agents triggers intracellular\nevents that activate ATP release.\nIn this thesis factors controlling the temporal evolution of extracellular ATP\n(ATPe) concentration were analyzed. Cells were exposed to two ATP release\nstimuli: 3V and the peptide mastoparán 7 (MST7). Both stimuli induce increases\nof cAMP, the main second messenger.\nMain results:\nUnder 3V exposure there is an increase of cAMP, which translated into an\nacute, transient release of ATP. When cells were pre-exposed to CBX\n(pannexin 1 inhibitor), ATP release induced by 3V was completely blocked.Thus\nunder 3V stimulation, RBCs exhibit a single ATP conduit mediated by pannexin\n1.\nUnder MST7 exposure, small cAMP increases correlated with a strong ATP\nrelease. CBX induced a partial blockage of ATP release, suggesting the\nexistence of two conduits for ATP release. In addition to cAMP, the potential\nrole of cell volume and cell adhesion on ATPe kinetics was studied.\nIn non-adhered cells, MST7 neither trigger ATP release nor affected cell\nvolume. On the other hand, in cells adhered to coverslips, and exposed to\nMST7, ATPe concentrations strongly increased and a volume increased was\nobserved. |
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