Caracterización de péptidos de la piel de anfibios de la Patagonia mediante técnicas de biología molecular. Su aplicación como antimicrobianos
The development of multidrug-resistant bacteria represents a threat to health, making the research for new therapeutic agents imperative. Antimicrobial peptides constitute a promising option. This thesis presents the first isolation and characterization of skin peptides from Pleurodema somuncurense...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2023
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7898 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7898.dir/7898.PDF |
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| Sumario: | The development of multidrug-resistant bacteria represents a threat to health, making the research for new therapeutic agents imperative. Antimicrobial peptides constitute a promising option.
This thesis presents the first isolation and characterization of skin peptides from Pleurodema somuncurense and Odontoprhynus occidentalis. RNA was extracted from the skin, and cDNA were obtained, transfected, and cloned into competent cells. Insert were selected, amplified, and sequenced. Through in silico analysis of the prepro-peptides, certain were selected for Fmoc chemistry synthesis. The peptides were purified by RP-HPLC/MSMS and tested for antimicrobial activity. Three peptides inhibited the growth of E. coli, and one inhibited the growth of S. aureus. Circular dichroism studies showed that two of them adopt ?-helix type structure under specific conditions. Hemolytic activity was determined and one showed low hemolysis percentage at the MIC. The results of several biophysical studies suggest a fusogenic mechanism of action leading to aggregation and alteration of cell morphology.
The sequences obtained enrich the databases that serve as a starting point in the design of new therapeutic agents against multidrug-resistant bacteria. |
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