Microsoft Word - Manuscrito 25-mar-14.docx

Dehydroleucodine (DhL) is a sesquiterpene lactone isolated from the leaves of the herb Artemisia douglasiana Besser, which grows in Argentina. Previously, our laboratory has shown that DhL inhibited the proliferation of tumor cells. In this work we analyzed the anti-migratory activity of DhL. As st...

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Autor principal: Amaya, Celina
Otros Autores: Davio, Carlos
Lenguaje:Español
Publicado: 2014
Materias:
Migración
Metástasis
Lactonas sesquiterpénicas
Deshidroleucodina
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afitesis&cl=CL1&d=HWA_783
https://repositoriouba.sisbi.uba.ar/gsdl/collect/afitesis/index/assoc/HWA_783.dir/783.PDF
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_783
record_format dspace
spelling I28-R145-HWA_7832024-12-20 Microsoft Word - Manuscrito 25-mar-14.docx Dehydroleucodine (DhL) is a sesquiterpene lactone isolated from the leaves of the herb Artemisia douglasiana Besser, which grows in Argentina. Previously, our laboratory has shown that DhL inhibited the proliferation of tumor cells. In this work we analyzed the anti-migratory activity of DhL. As study models, tumor cells in culture (HeLa, B16-F0, B16-F10, MCF-7 and MDA-MB-231), arrested in G1/S phase of the cell cycle, were used. In cells treated with DhL were analyzed the properties of adhesion, invasion and migration. DhL did not induce apoptosis or senescence and neither affected the cell viability. DhL inhibited the matrigel invasion and the migration in a wound healing assay. DhL inhibited the membrane protrusion in the cell front due to affected the organization of actin cytoskeleton and inhibited the signalling proteins as Rac1, ERK and p38. These results indicated that DhL, affects the adhesion, invasion and migration of tumor cells in culture due to the inhibition of ERK signalling pathway. This work provides valuable information to consider DhL as a potential pharmacology drug to be used as a antimetastatic agent in cancer therapies. Fil: Amaya, Celina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Davio, Carlos López, Luis Alberto Amaya, Celina 2014-05-27 Deshidroleucodina (DhL) es una lactona sesquiterpénica aislada de las hojas de la planta Artemisia douglasiana Besser, que crece en Argentina. Previamente, nuestro laboratorio ha demostrado que DhL inhibe la proliferación de células tumorales en cultivo y el crecimiento de tumores en ratones. Con el fin de demostrar que DhL posee potencial para el desarrollo de fármacos antitumorales, en esta tesis analizamos las propiedades antimigratorias de DhL en células tumorales. Como modelo de estudio utilizamos células en cultivo, arrestadas en la fase G1/S del ciclo celular. En las células tratadas con DhL fueron analizadas las propiedades de adhesión, invasión y migración. Como resultado, observamos que DhL no indujo apoptosis ni senescencia y no afectó la viabilidad celular. Luego, en una superficie recubierta con fibronectina, comprobamos que DhL inhibió la adhesión y la formación de adhesiones focales. Posteriormente, observamos que DhL inhibió la invasión del matrigel por las células tumorales y la migración celular. Además, DhL inhibió la protrusión de la membrana en el frente de avance, por la alteración de la organización del citoesqueleto de actina y la actividad de proteínas señaladotas como Rac1, ERK y p38. Estos resultados indican que DhL, a través de la inhibición de la señalización de ERK, afecta la adhesión, invasión y migración de las células tumorales en cultivo, confirmando la hipótesis que esta lactona sesquiterpénica tiene un gran potencial farmacológico como agente antimetastásico. Moreno, Silvia Simian, Marina Genaro, Ana Migración Metástasis Lactonas sesquiterpénicas Deshidroleucodina spa Efecto inhibitorio de Deshidroleucodina en la migración e invasión de las células tumorales en cultivo http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afitesis&cl=CL1&d=HWA_783 https://repositoriouba.sisbi.uba.ar/gsdl/collect/afitesis/index/assoc/HWA_783.dir/783.PDF
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic Migración
Metástasis
Lactonas sesquiterpénicas
Deshidroleucodina
spellingShingle Migración
Metástasis
Lactonas sesquiterpénicas
Deshidroleucodina
Amaya, Celina
Microsoft Word - Manuscrito 25-mar-14.docx
topic_facet Migración
Metástasis
Lactonas sesquiterpénicas
Deshidroleucodina
description Dehydroleucodine (DhL) is a sesquiterpene lactone isolated from the leaves of the herb Artemisia douglasiana Besser, which grows in Argentina. Previously, our laboratory has shown that DhL inhibited the proliferation of tumor cells. In this work we analyzed the anti-migratory activity of DhL. As study models, tumor cells in culture (HeLa, B16-F0, B16-F10, MCF-7 and MDA-MB-231), arrested in G1/S phase of the cell cycle, were used. In cells treated with DhL were analyzed the properties of adhesion, invasion and migration. DhL did not induce apoptosis or senescence and neither affected the cell viability. DhL inhibited the matrigel invasion and the migration in a wound healing assay. DhL inhibited the membrane protrusion in the cell front due to affected the organization of actin cytoskeleton and inhibited the signalling proteins as Rac1, ERK and p38. These results indicated that DhL, affects the adhesion, invasion and migration of tumor cells in culture due to the inhibition of ERK signalling pathway. This work provides valuable information to consider DhL as a potential pharmacology drug to be used as a antimetastatic agent in cancer therapies.
author2 Davio, Carlos
author_facet Davio, Carlos
Amaya, Celina
author Amaya, Celina
author_sort Amaya, Celina
title Microsoft Word - Manuscrito 25-mar-14.docx
title_short Microsoft Word - Manuscrito 25-mar-14.docx
title_full Microsoft Word - Manuscrito 25-mar-14.docx
title_fullStr Microsoft Word - Manuscrito 25-mar-14.docx
title_full_unstemmed Microsoft Word - Manuscrito 25-mar-14.docx
title_sort microsoft word - manuscrito 25-mar-14.docx
publishDate 2014
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afitesis&cl=CL1&d=HWA_783
https://repositoriouba.sisbi.uba.ar/gsdl/collect/afitesis/index/assoc/HWA_783.dir/783.PDF
work_keys_str_mv AT amayacelina microsoftwordmanuscrito25mar14docx
AT amayacelina efectoinhibitoriodedeshidroleucodinaenlamigracioneinvasiondelascelulastumoralesencultivo
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