Proteólisis en plantas bajo condiciones de estrés producidas por metales
Plant proteolytic system includes proteases, mainly localized inside the organelles, and the ubiquitin-proteasome pathway in both, the cytoplasm and the nucleus. In cotyledons and leaves of sunflower plants treated with AlCl3, CoCl2, CuCl2, CrCl3, HgCl2, NiCl2, PbCl2 or ZnCl2 were produced for prote...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2009
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7823 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7823.dir/7823.PDF |
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I28-R145-HWA_78232025-08-01 Plant proteolytic system includes proteases, mainly localized inside the organelles, and the ubiquitin-proteasome pathway in both, the cytoplasm and the nucleus. In cotyledons and leaves of sunflower plants treated with AlCl3, CoCl2, CuCl2, CrCl3, HgCl2, NiCl2, PbCl2 or ZnCl2 were produced for protein oxidative damage. It is concluded that impairment of proteasome functionality and altered proteases activities seem to be a common feature involved in metal phytotoxicity, but heavy metal effects on proteolytic system cannot be generalized. Particularly, cadmium increased protease specific activity in sunflower cotyledons and leaves; nevertheless, this increase was not relevant enough to avoid accumulation of oxidized proteins. Oxidation of proteins is one of the most important effects of cadmium treatment. Leaves maize treatment with 50 -M CdCl2 increased two of the three 20S proteasome activities. The incremental changes in proteasome activities were probably caused by an increased level of proteasome oxidation, with this being responsible for degradation of oxidized proteins. Leaf treated with 100 -M CdCl2 decreased two 20S proteasome activities, with a concomitant increase in accumulation of carbonylated and ubiquitinated proteins. These results confirm evidence for the involvement of this proteolytic system in cadmium-stressed plants. Fil: Pena, Liliana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Gallego, Susana Mabel Pena, Liliana Beatriz 2009-08-14 El sistema proteolítico de plantas está compuesto por proteasas, localizadas principalmente en organelas, y la ruta ubicuitina-proteasoma en citoplasma y núcleo. En cotiledones y hojas de girasol tratados con AlCl3, CoCl2, CuCl2, CrCl3, HgCl2, NiCl2, PbCl2 o ZnCl2 se produjo daño oxidativo a proteínas. El deterioro de la funcionalidad del proteasoma y la alteración de la actividad de proteasas parecen ser una característica común en la fitotoxicidad por metales, si bien los efectos de los mismos no se pueden generalizar. El cadmio aumentó la actividad de proteasas específicas en cotiledones y hojas de girasol, pero este incremento no evitó la acumulación de proteínas oxidadas, uno de los efectos más importantes de este tipo de estrés. El tratamiento de segmentos de hoja de maíz con 50 -M CdCl2 aumentó dos de las tres actividades del proteasoma 20S. Este incremento fue causado probablemente por un aumento en el nivel de oxidación del mismo, que sería responsable de la degradación de proteínas oxidadas. Hojas tratadas con 100 -M CdCl2 disminuyeron dos de las actividades del proteasoma 20S, con el consiguiente aumento de la acumulación de proteínas carboniladas y ubicuitinadas. Estos resultados confirman la participación de este sistema proteolítico en estrés por cadmio en plantas. application/pdf Gurni, Alberto Verstraeten, Sandra Civello, Pedro Proteasas Proteasoma Metales Cadmio Proteínas oxidadas Proteases Proteasome Metals Cadmium Oxidized proteins spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Doctora de la Universidad de Buenos Aires en Bioquímica Vegetal Proteólisis en plantas bajo condiciones de estrés producidas por metales info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7823 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7823.dir/7823.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Proteasas Proteasoma Metales Cadmio Proteínas oxidadas Proteases Proteasome Metals Cadmium Oxidized proteins Ciencias de la vida |
| spellingShingle |
Proteasas Proteasoma Metales Cadmio Proteínas oxidadas Proteases Proteasome Metals Cadmium Oxidized proteins Ciencias de la vida Pena, Liliana Beatriz Proteólisis en plantas bajo condiciones de estrés producidas por metales |
| topic_facet |
Proteasas Proteasoma Metales Cadmio Proteínas oxidadas Proteases Proteasome Metals Cadmium Oxidized proteins Ciencias de la vida |
| description |
Plant proteolytic system includes proteases, mainly localized inside the
organelles, and the ubiquitin-proteasome pathway in both, the cytoplasm and the
nucleus. In cotyledons and leaves of sunflower plants treated with AlCl3, CoCl2,
CuCl2, CrCl3, HgCl2, NiCl2, PbCl2 or ZnCl2 were produced for protein oxidative
damage. It is concluded that impairment of proteasome functionality and altered
proteases activities seem to be a common feature involved in metal phytotoxicity,
but heavy metal effects on proteolytic system cannot be generalized.
Particularly, cadmium increased protease specific activity in sunflower
cotyledons and leaves; nevertheless, this increase was not relevant enough to
avoid accumulation of oxidized proteins. Oxidation of proteins is one of the most
important effects of cadmium treatment. Leaves maize treatment with 50 -M
CdCl2 increased two of the three 20S proteasome activities. The incremental
changes in proteasome activities were probably caused by an increased level of
proteasome oxidation, with this being responsible for degradation of oxidized
proteins. Leaf treated with 100 -M CdCl2 decreased two 20S proteasome activities,
with a concomitant increase in accumulation of carbonylated and ubiquitinated
proteins. These results confirm evidence for the involvement of this proteolytic
system in cadmium-stressed plants. |
| author2 |
Gallego, Susana Mabel |
| author_facet |
Gallego, Susana Mabel Pena, Liliana Beatriz |
| format |
Tesis doctoral Tesis doctoral acceptedVersion |
| author |
Pena, Liliana Beatriz |
| author_sort |
Pena, Liliana Beatriz |
| title |
Proteólisis en plantas bajo condiciones de estrés producidas por metales |
| title_short |
Proteólisis en plantas bajo condiciones de estrés producidas por metales |
| title_full |
Proteólisis en plantas bajo condiciones de estrés producidas por metales |
| title_fullStr |
Proteólisis en plantas bajo condiciones de estrés producidas por metales |
| title_full_unstemmed |
Proteólisis en plantas bajo condiciones de estrés producidas por metales |
| title_sort |
proteólisis en plantas bajo condiciones de estrés producidas por metales |
| publisher |
Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica |
| publishDate |
2009 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7823 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7823.dir/7823.PDF |
| work_keys_str_mv |
AT penalilianabeatriz proteolisisenplantasbajocondicionesdeestresproducidaspormetales |
| _version_ |
1840330196018790400 |