“Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta”

Small Ruminant Lentiviruses (SRLV) are present worldwide and recently described in Buenos \nAires province in 2017, however extension and infection importance in the Salta province is \nunknown. This virus infection can present four classic clinical forms: articular, mammary, \npulmonary and nervous...

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Autor principal: Dodero, Ana Mariela
Otros Autores: Reina Arias, Ramsés
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Universidad de Buenos Aires. Facultad de Ciencias Veterinarias 2023
Materias:
SRLV
CAEV
Caracterización molecular
Epidemiologia
Aislamiento
Salta
Molecula Character
Epidemiology
Isolation
Artritis
Encefalitis
Ciencias Veterinarias
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_7239
https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_7239.dir/7239.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_7239
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic SRLV
CAEV
Caracterización molecular
Epidemiologia
Aislamiento
Salta
SRLV
CAEV
Molecula Character
Epidemiology
Isolation
Salta
Artritis
Encefalitis
Ciencias Veterinarias
spellingShingle SRLV
CAEV
Caracterización molecular
Epidemiologia
Aislamiento
Salta
SRLV
CAEV
Molecula Character
Epidemiology
Isolation
Salta
Artritis
Encefalitis
Ciencias Veterinarias
Dodero, Ana Mariela
“Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta”
topic_facet SRLV
CAEV
Caracterización molecular
Epidemiologia
Aislamiento
Salta
SRLV
CAEV
Molecula Character
Epidemiology
Isolation
Salta
Artritis
Encefalitis
Ciencias Veterinarias
description Small Ruminant Lentiviruses (SRLV) are present worldwide and recently described in Buenos \nAires province in 2017, however extension and infection importance in the Salta province is \nunknown. This virus infection can present four classic clinical forms: articular, mammary, \npulmonary and nervous. This disease causes numerous production losses such as those \nresulting from reduced feed intake due to the pain of the arthritic pathology; the drop in milk \nproduction, which is also justified by mastitis that leads to early drying of the animal and loss \nof the mammary means; deaths caused by encephalitis and pulmonary diseases added to the \nimpact of premature discards induced by the virus. The aim of the study was to identify, isolate \nand molecularly characterize the SRLVs present in the Salta province in order to detect the \ncirculating strains and develop diagnostic methods to be applied in control programs. About \n927 adult animals were sampled from 32 herds distributed in three ecoregions, Chaco Salteño \n(CS), Valles y Quebradas áridas (VA) and Valles Templados (VT), together with a socio-economic and productive survey at each site. One of the farms was used as an experimental \nherd for biological sampling, practice of management measures and clinical follow-up of a \ncontrol case. Herds were screened with commercial indirect ELISAs and concordance tests \nwere performed. The overall seroprevalence was 8.26% and by ecoregion: 9.17% in CS, \n6.48% in VA and 11.35% in VT. Genomics sequences were obtained from the gag region, \nwhich allowed the molecular characterization of the strains as a B1 subtype, the development \nof specific diagnostic PCRs, and the expression and purification of the viral recombinant \nprotein p25, followed by the first steps in the evaluation of its potential use in an indirect ELISA. \nPrimary sheep and goat cultures were produced, characterised and challenged with viral \ninocula obtaining clear cytopathic effects, but unsuccessful in isolating infective strains. Based \non the survey data, risk factor analyses for the presence of the infection were carried out, being \nmore risky if the herd is not dewormed, mechanically milked or collectively bred. Finally, a \nmanagement protocol was proposed for the control of SRLVs based on the early identification \nof infected animals and the selection of uninfected animals for restocking.\nThe results reflect the presence of SRLVs in the Salta province, probably derived from animal \nimportations; the importance of livestock activity in the area studied; and the need to develop specific surveillance plans for SRLV control, thus avoiding the spread of the infection.
author2 Reina Arias, Ramsés
author_facet Reina Arias, Ramsés
Dodero, Ana Mariela
format Tesis doctoral
Tesis doctoral
acceptedVersion
author Dodero, Ana Mariela
author_sort Dodero, Ana Mariela
title “Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta”
title_short “Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta”
title_full “Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta”
title_fullStr “Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta”
title_full_unstemmed “Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta”
title_sort “identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de salta”
publisher Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
publishDate 2023
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_7239
https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_7239.dir/7239.PDF
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spelling I28-R145-HWA_72392024-08-27 “Identificacin, aislamiento y caracterizacin del virus de artritis y encefalitis caprina en la provincia de Salta” Small Ruminant Lentiviruses (SRLV) are present worldwide and recently described in Buenos \nAires province in 2017, however extension and infection importance in the Salta province is \nunknown. This virus infection can present four classic clinical forms: articular, mammary, \npulmonary and nervous. This disease causes numerous production losses such as those \nresulting from reduced feed intake due to the pain of the arthritic pathology; the drop in milk \nproduction, which is also justified by mastitis that leads to early drying of the animal and loss \nof the mammary means; deaths caused by encephalitis and pulmonary diseases added to the \nimpact of premature discards induced by the virus. The aim of the study was to identify, isolate \nand molecularly characterize the SRLVs present in the Salta province in order to detect the \ncirculating strains and develop diagnostic methods to be applied in control programs. About \n927 adult animals were sampled from 32 herds distributed in three ecoregions, Chaco Salteño \n(CS), Valles y Quebradas áridas (VA) and Valles Templados (VT), together with a socio-economic and productive survey at each site. One of the farms was used as an experimental \nherd for biological sampling, practice of management measures and clinical follow-up of a \ncontrol case. Herds were screened with commercial indirect ELISAs and concordance tests \nwere performed. The overall seroprevalence was 8.26% and by ecoregion: 9.17% in CS, \n6.48% in VA and 11.35% in VT. Genomics sequences were obtained from the gag region, \nwhich allowed the molecular characterization of the strains as a B1 subtype, the development \nof specific diagnostic PCRs, and the expression and purification of the viral recombinant \nprotein p25, followed by the first steps in the evaluation of its potential use in an indirect ELISA. \nPrimary sheep and goat cultures were produced, characterised and challenged with viral \ninocula obtaining clear cytopathic effects, but unsuccessful in isolating infective strains. Based \non the survey data, risk factor analyses for the presence of the infection were carried out, being \nmore risky if the herd is not dewormed, mechanically milked or collectively bred. Finally, a \nmanagement protocol was proposed for the control of SRLVs based on the early identification \nof infected animals and the selection of uninfected animals for restocking.\nThe results reflect the presence of SRLVs in the Salta province, probably derived from animal \nimportations; the importance of livestock activity in the area studied; and the need to develop specific surveillance plans for SRLV control, thus avoiding the spread of the infection. Fil: Dodero, Ana Mariela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Buenos Aires, Argentina Reina Arias, Ramsés Peralta, Andrea Verónica Dodero, Ana Mariela 2023-09-08 Los Lentivirus de Pequeños Rumiantes (SRLVs) están presentes en todo el mundo y se ha \ndescrito su presencia en la provincia de Buenos Aires en 2017, sin embargo, se desconoce la \nextensión de la infección y su importancia en la provincia de Salta. La infección por este virus \npuede presentar cuatro formas clínicas clásicas: articular, mamaria, pulmonar y nerviosa. La\nenfermedad causa numerosas pérdidas productivas como las derivadas de una menor ingesta \nde alimentos debido al dolor de la patología artrítica; la baja de la producción láctea que \ntambién está justificada por las mastitis que llegan hasta el secado precoz del animal y pérdida \nde los medios mamarios; las muertes producidas por encefalitis y afecciones pulmonares\nsumadas al impacto de los descartes prematuros inducidos por la virosis. El objetivo del \ntrabajo fue identificar, aislar y caracterizar molecularmente los SRLVs presentes en la \nprovincia de Salta para detectar las estirpes circulantes y desarrollar métodos diagnósticos \ncon los que diseñar planes de control. Se muestrearon 927 animales adultos de 32 hatos \ndistribuidos en tres ecorregiones, Chaco Salteño (CS), Valles y Quebradas Áridas (QA) y \nValles Templados (VT), acompañados por una encuesta socioeconómica y productiva de cada \nlugar. Uno de los establecimientos se utilizó como rebaño experimental para la toma de \nmuestras biológicas, práctica de medidas de manejo y seguimiento clínico de un caso testigo. \nSe cribaron los hatos con ELISAs indirectos comerciales y se realizaron pruebas de \nconcordancia entre ellos. La seroprevalencia general fue de 8,26% y por ecorregiones fue de \n9,17% en CS, 6,48% en QA y 11,35% en VT. Se obtuvieron secuencias genómicas de la \nregión gag que permitieron la caracterización molecular de las cepas como subtipo B1, el \ndesarrollo de PCRs diagnósticas específicas y la expresión y purificación de la proteína \nrecombinante viral p25, seguida de la realización de los primeros pasos en la evaluación de su uso potencial en un ELISA indirecto. Se produjeron y caracterizaron cultivos primarios de \norigen ovino y caprino y se desafiaron con inóculos virales obteniéndose efectos citopáticos, \nsin éxito a la hora de aislar estirpes virales infectivas. A partir de los datos de la encuesta, se \nrealizó un análisis de factores de riesgo para la presencia de la infección, hallando un riesgo \nmayor si el hato no realiza desparasitaciones o emplea ordeñe mecánico y crianza en guachera colectiva. Finalmente, se propuso un protocolo de manejo para el control de los \nSRLVs basado en la identificación temprana de los animales infectados y elección de los \nindemnes para la reposición.\nLos resultados reflejan la presencia de SRLV en la provincia de Salta probablemente derivada \nde la importación de animales; la importancia de la actividad ganadera en la zona estudiada; \ny la necesidad de elaborar planes de vigilancia específicos para su control, evitando así la diseminación de la infección. application/pdf SRLV CAEV Caracterización molecular Epidemiologia Aislamiento Salta SRLV CAEV Molecula Character Epidemiology Isolation Salta spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess htpps://creativecommons.org/licenses/by-nend/2.5/ar/ Artritis Encefalitis Ciencias Veterinarias Doctora de Universidad de Buenos Aires en Ciencias Veterinarias Identificación, aislamiento y caracterización del virus de artritis y enecefalitis caprina den la provincia de Salta info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_7239 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_7239.dir/7239.PDF

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