7062

The franciscana dolphin, Pontoporia blainvillei, is a small cetacean with marine and coastal habits. Its distribution extends from Espíritu Santo (Brasil) to Río Negro (Argentina). It is\nclassified as Vulnerable by IUCN. Previous studies found genetically distinct populations along its distribution...

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Autor principal: Gariboldi, María Constanza
Otros Autores: Capozzo, Humberto Luis
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Universidad de Buenos Aires. Facultad de Ciencias Veterinarias 2017
Materias:
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_7062
https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_7062.dir/7062.PDF
Aporte de:
id I28-R145-HWA_7062
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic ADN mitocondrial
Microsatélites
Estructura genética poblacional
Franciscana dolphin
Mitochondrial DNA
Microsatellites
Population genetic
Social structure
Delfín franciscana
Genética
Estructura social
Distribución geográfica
Ciencias Veterinarias
spellingShingle ADN mitocondrial
Microsatélites
Estructura genética poblacional
Franciscana dolphin
Mitochondrial DNA
Microsatellites
Population genetic
Social structure
Delfín franciscana
Genética
Estructura social
Distribución geográfica
Ciencias Veterinarias
Gariboldi, María Constanza
7062
topic_facet ADN mitocondrial
Microsatélites
Estructura genética poblacional
Franciscana dolphin
Mitochondrial DNA
Microsatellites
Population genetic
Social structure
Delfín franciscana
Genética
Estructura social
Distribución geográfica
Ciencias Veterinarias
description The franciscana dolphin, Pontoporia blainvillei, is a small cetacean with marine and coastal habits. Its distribution extends from Espíritu Santo (Brasil) to Río Negro (Argentina). It is\nclassified as Vulnerable by IUCN. Previous studies found genetically distinct populations along its distribution; although the southernmost portion of its rage still lacks information.\nThe goal of this thesis was to improve the knowledge about the population genetic identity, genetic variability and social structure of the species in the southern edge of its distribution. Samples from 74 individuals were collected from incidentally entangled or stranded franciscanas along the coastal area between Necochea and Río Negro; 5 samples from San Clemente del Tuyú and Pinamar were collected as well A fragment from the mitochondrial DNA control region, 10 microsatellite loci and fragments from ZFX and SRY were amplified.In the southern area of the species´ distribution, genetic information was combined with environmental and age data. Five novel mitochondrial haplotypes were found, totalizing 60\nhaplotypes described for the whole distribution area of the species. Higher levels of mitochondrial DNA diversity suggest a species´ colonization from La Plata Estuary towards\nthe extremes of its distribution. As in other areas of the species´ distribution, no evidence of\nsex-biased dispersal was observed in the southern area of its distribution. Three genetically distinct populations were found in the southernmost portion of the franciscana distribution,\ntotalizing 5 populations within Argentina and 10 along its distribution. The population from\nRío de Janeiro would be the only one that experienced a sudden expansion 60.000 years ago. An isolation by distance pattern exists when considering the entire distribution of the species,but not in the southern area. The population genetic structure in the latter area would not be explained by environmental data either; other factors might affect the population genetic\nstructure in the southernmost area of the species distribution. Also, random mating between\nindividuals and a lack of population substructure would exist; however, relatedness between\nindividuals within each population is significantly different than that observed between populations. This is the first study of the species where, within the same fish net, a father and\nits two same age offsprings where found: male franciscanas might prolong their bonds with\ntheir offspring at least until the first year of the offspring life, and the mating system might change in space and/or time. Results achieved in this thesis increase the population genetic and social structure knowledge of the species, which can be used to develop management and\nconservation plans that ensure the log-term survival of the species
author2 Capozzo, Humberto Luis
author_facet Capozzo, Humberto Luis
Gariboldi, María Constanza
format Tesis doctoral
Tesis doctoral
acceptedVersion
author Gariboldi, María Constanza
author_sort Gariboldi, María Constanza
title 7062
title_short 7062
title_full 7062
title_fullStr 7062
title_full_unstemmed 7062
title_sort 7062
publisher Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
publishDate 2017
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_7062
https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_7062.dir/7062.PDF
work_keys_str_mv AT gariboldimariaconstanza 7062
AT gariboldimariaconstanza identidadgeneticayestructurasocialdeldelfinfranciscanaportoporiablainvilleienelareasurdesudistribucion
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spelling I28-R145-HWA_70622024-08-27 7062 The franciscana dolphin, Pontoporia blainvillei, is a small cetacean with marine and coastal habits. Its distribution extends from Espíritu Santo (Brasil) to Río Negro (Argentina). It is\nclassified as Vulnerable by IUCN. Previous studies found genetically distinct populations along its distribution; although the southernmost portion of its rage still lacks information.\nThe goal of this thesis was to improve the knowledge about the population genetic identity, genetic variability and social structure of the species in the southern edge of its distribution. Samples from 74 individuals were collected from incidentally entangled or stranded franciscanas along the coastal area between Necochea and Río Negro; 5 samples from San Clemente del Tuyú and Pinamar were collected as well A fragment from the mitochondrial DNA control region, 10 microsatellite loci and fragments from ZFX and SRY were amplified.In the southern area of the species´ distribution, genetic information was combined with environmental and age data. Five novel mitochondrial haplotypes were found, totalizing 60\nhaplotypes described for the whole distribution area of the species. Higher levels of mitochondrial DNA diversity suggest a species´ colonization from La Plata Estuary towards\nthe extremes of its distribution. As in other areas of the species´ distribution, no evidence of\nsex-biased dispersal was observed in the southern area of its distribution. Three genetically distinct populations were found in the southernmost portion of the franciscana distribution,\ntotalizing 5 populations within Argentina and 10 along its distribution. The population from\nRío de Janeiro would be the only one that experienced a sudden expansion 60.000 years ago. An isolation by distance pattern exists when considering the entire distribution of the species,but not in the southern area. The population genetic structure in the latter area would not be explained by environmental data either; other factors might affect the population genetic\nstructure in the southernmost area of the species distribution. Also, random mating between\nindividuals and a lack of population substructure would exist; however, relatedness between\nindividuals within each population is significantly different than that observed between populations. This is the first study of the species where, within the same fish net, a father and\nits two same age offsprings where found: male franciscanas might prolong their bonds with\ntheir offspring at least until the first year of the offspring life, and the mating system might change in space and/or time. Results achieved in this thesis increase the population genetic and social structure knowledge of the species, which can be used to develop management and\nconservation plans that ensure the log-term survival of the species Fil: Gariboldi, Marìa Constanza. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Buenos Aires, Argentina Capozzo, Humberto Luis Gariboldi, María Constanza 2017-03-20 El delfín franciscana, Pontoporia blainvillei, es un pequeño cetáceo de hábitos marinocosteros que se distribuye desde Espíritu Santo (Brasil) a Río Negro (Argentina). Está clasificado como Vulnerable por la IUCN. Estudios previos hallaron poblaciones genéticamente diferenciadas a lo largo de su distribución; sin embargo, aún faltan datos de su extremo más austral. El objetivo de esta tesis fue generar nuevo conocimiento sobre la identidad genética poblacional, la variabilidad genética y la estructura social de la especie en\nel extremo sur de su distribución. Se utilizaron muestras de 74 individuos capturados de forma incidental en redes de pesca costera y artesanal o hallados muertos en la playa, en el área comprendida entre Necochea y Río Negro; además, se colectaron 5 individuos de San\nClemente del Tuyú y Pinamar. Se amplificó un fragmento de la región control del ADN\nmitocondrial, 10 loci de microsatélites y segmentos de los genes ZFX y SRY. En el área sur de la distribución de la especie, la información se analizó en conjunto con datos ambientales y la\nedad. Se hallaron 5 nuevos haplotipos mitocondriales, totalizando 60 haplotipos descriptos para toda el área de distribución de la especie. La mayor diversidad genética mitocondrial hallada en áreas cercanas al estuario del Río de la Plata sugiere una colonización de la especie desde el Estuario del Río de la Plata hacia los extremos de distribución. Al igual que en otras\náreas de distribución de la especie, en el área sur no se hallaron evidencias de un sesgo en la dispersión relacionado al sexo. Se hallaron 3 poblaciones genéticamente diferenciadas en el extremo sur de distribución, totalizando 5 poblaciones en Argentina y 10 a lo largo de su\ndistribución. Río de Janeiro es la única población que habría experimentado un fenómeno de expansión repentino hace 60.000 años. Considerando toda el área de distribución de la especie, existe un patrón de aislamiento por distancia; este patrón no fue observado en el área\nsur de su distribución. En esta área tampoco las variables ambientales influirían en la\nestructuración genética poblacional de la especie; otros factores afectarían la estructura poblacional en esta área. Por otro lado, existiría un apareamiento al azar entre los individuos\ny no habría una subestructuración poblacional; aunque, el parentesco entre individuos dentro de cada población es significativamente diferente al observado ente poblaciones. Este es el primer estudio sobre la especie en el que se halla en una misma red de pesca a un macho, padre de 2 crías de igual edad: los machos prolongarían el vínculo con sus crías, al menos, hasta el primer año de vida de éstas y el sistema de apareamiento podría variar en el tiempo\ny/o el espacio. Los resultados de esta tesis permiten incrementar el conocimiento acerca de la\nestructura genética poblacional y social del delfín franciscana que podrá ser utilizado para\nestablecer medidas de manejo y conservación adecuadas que aseguren la supervivencia a\nlargo plazo de esta especie. application/pdf ADN mitocondrial Microsatélites Estructura genética poblacional Franciscana dolphin Mitochondrial DNA Microsatellites Population genetic Social structure spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-ncnd/2.5/ar/ Delfín franciscana Genética Estructura social Distribución geográfica Ciencias Veterinarias Genética poblacional Doctora de la Universidad de Buenos Aires en Ciencias Veterinarias Identidad genética y estructura social del delfín Franciscana, Portoporia blainvillei, en el área sur de su distribución info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_7062 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_7062.dir/7062.PDF