Maestra en Salud Animal
Copper deficiency is an important disease of cattle widely distributed worldwide. This deficiency can be primary or secondary. Copper is a cofactor of numerous enzymes, therefore its deficiency generates various injuries. One manifestation of copper deficiency in bovines is the falling disease, char...
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| Formato: | Tesis de maestría acceptedVersion |
| Lenguaje: | Español |
| Publicado: |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2019
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avemaster&cl=CL1&d=HWA_7020 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avemaster/index/assoc/HWA_7020.dir/7020.PDF |
| Aporte de: |
| Sumario: | Copper deficiency is an important disease of cattle widely distributed worldwide. This deficiency can be primary or secondary. Copper is a cofactor of numerous enzymes, therefore its deficiency generates various injuries. One manifestation of copper deficiency in bovines is the falling disease, characterized by heart lesions that lead to heart failure. The\naim of this thesis is to demonstrate that there are changes in the microscopic composition of\nthe basement membranes of the heart in cattle with secondary copper deficiency induced by consumption of diets with high levels of molybdenum and sulfur, caused by a decrease in the activity of the enzyme lysyl oxidase. We used 18 myocardial samples from the right ventricle that come from 2 experimental trials with a total of 18 Holando Argentino calves, of 60 days\nthat were divided into 2 groups (control group and deficient group). The samples were preserved in paraffin blocks and cut to 5 µm to perform the picrosirius network histochemical technique and immunohistochemistry for type IV collagen and laminin. Other samples of\nmyocardium were frozen at -70 ° C to measure the activity of the enzyme lysyl oxidase. No differences were observed in picrosirius red histochemical staining or in immunohistochemistry for type IV collagen and laminin of the heart. The concentration of active LOX (estimated from its activity) in the animals of the deficient group was 0.97 ± 0.29 ?g LOX / mg protein, while in the animals of the control group it was 1.56 ± 0, 60 ?g LOX /mg protein, being the difference statistically significant (p = 0.03). It is concluded that cattle\nwith copper deficiency secondary to high levels of molybdenum and sulfur in the diet have a decrease activity of the enzyme lysyl oxidase at the cardiac level. The absence of significant morphologic alterations in connective tissue and basement membranes in this work could be associated with the duration of the deficiency. We believe that the data obtained in this thesis will contribute to the knowledge of the pathogenesis suffered by hearts in animals with falling\ndisease. |
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