Maestra en Salud Animal
Copper deficiency is an important disease of cattle widely distributed worldwide. This deficiency can be primary or secondary. Copper is a cofactor of numerous enzymes, therefore its deficiency generates various injuries. One manifestation of copper deficiency in bovines is the falling disease, char...
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| Formato: | Tesis de maestría acceptedVersion |
| Lenguaje: | Español |
| Publicado: |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2019
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avemaster&cl=CL1&d=HWA_7020 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avemaster/index/assoc/HWA_7020.dir/7020.PDF |
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I28-R145-HWA_7020 |
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dspace |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
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spa |
| topic |
Bovinos Deficiencia de cobre Tejido conectivo Bovine Copper deficiency Tissue connective Ciencias Veterinarias |
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Bovinos Deficiencia de cobre Tejido conectivo Bovine Copper deficiency Tissue connective Ciencias Veterinarias Nicastro, Carolina Natalia Maestra en Salud Animal |
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Bovinos Deficiencia de cobre Tejido conectivo Bovine Copper deficiency Tissue connective Ciencias Veterinarias |
| description |
Copper deficiency is an important disease of cattle widely distributed worldwide. This deficiency can be primary or secondary. Copper is a cofactor of numerous enzymes, therefore its deficiency generates various injuries. One manifestation of copper deficiency in bovines is the falling disease, characterized by heart lesions that lead to heart failure. The\naim of this thesis is to demonstrate that there are changes in the microscopic composition of\nthe basement membranes of the heart in cattle with secondary copper deficiency induced by consumption of diets with high levels of molybdenum and sulfur, caused by a decrease in the activity of the enzyme lysyl oxidase. We used 18 myocardial samples from the right ventricle that come from 2 experimental trials with a total of 18 Holando Argentino calves, of 60 days\nthat were divided into 2 groups (control group and deficient group). The samples were preserved in paraffin blocks and cut to 5 µm to perform the picrosirius network histochemical technique and immunohistochemistry for type IV collagen and laminin. Other samples of\nmyocardium were frozen at -70 ° C to measure the activity of the enzyme lysyl oxidase. No differences were observed in picrosirius red histochemical staining or in immunohistochemistry for type IV collagen and laminin of the heart. The concentration of active LOX (estimated from its activity) in the animals of the deficient group was 0.97 ± 0.29 ?g LOX / mg protein, while in the animals of the control group it was 1.56 ± 0, 60 ?g LOX /mg protein, being the difference statistically significant (p = 0.03). It is concluded that cattle\nwith copper deficiency secondary to high levels of molybdenum and sulfur in the diet have a decrease activity of the enzyme lysyl oxidase at the cardiac level. The absence of significant morphologic alterations in connective tissue and basement membranes in this work could be associated with the duration of the deficiency. We believe that the data obtained in this thesis will contribute to the knowledge of the pathogenesis suffered by hearts in animals with falling\ndisease. |
| author2 |
Postma, Gabriela |
| author_facet |
Postma, Gabriela Nicastro, Carolina Natalia |
| format |
Tesis de maestría Tesis de maestría acceptedVersion |
| author |
Nicastro, Carolina Natalia |
| author_sort |
Nicastro, Carolina Natalia |
| title |
Maestra en Salud Animal |
| title_short |
Maestra en Salud Animal |
| title_full |
Maestra en Salud Animal |
| title_fullStr |
Maestra en Salud Animal |
| title_full_unstemmed |
Maestra en Salud Animal |
| title_sort |
maestra en salud animal |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias |
| publishDate |
2019 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avemaster&cl=CL1&d=HWA_7020 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avemaster/index/assoc/HWA_7020.dir/7020.PDF |
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AT nicastrocarolinanatalia maestraensaludanimal AT nicastrocarolinanatalia efectosdeladeficienciadecobreinducidaporaltosnivelesdemolibdenoyazufreenladietasobreeltejidoconectivodelendomisioyperimisioylasmembranasyperimisioylasmembranasbasalesdelcorazondebovinos |
| _version_ |
1824356458613964800 |
| spelling |
I28-R145-HWA_70202024-11-12 Maestra en Salud Animal Copper deficiency is an important disease of cattle widely distributed worldwide. This deficiency can be primary or secondary. Copper is a cofactor of numerous enzymes, therefore its deficiency generates various injuries. One manifestation of copper deficiency in bovines is the falling disease, characterized by heart lesions that lead to heart failure. The\naim of this thesis is to demonstrate that there are changes in the microscopic composition of\nthe basement membranes of the heart in cattle with secondary copper deficiency induced by consumption of diets with high levels of molybdenum and sulfur, caused by a decrease in the activity of the enzyme lysyl oxidase. We used 18 myocardial samples from the right ventricle that come from 2 experimental trials with a total of 18 Holando Argentino calves, of 60 days\nthat were divided into 2 groups (control group and deficient group). The samples were preserved in paraffin blocks and cut to 5 µm to perform the picrosirius network histochemical technique and immunohistochemistry for type IV collagen and laminin. Other samples of\nmyocardium were frozen at -70 ° C to measure the activity of the enzyme lysyl oxidase. No differences were observed in picrosirius red histochemical staining or in immunohistochemistry for type IV collagen and laminin of the heart. The concentration of active LOX (estimated from its activity) in the animals of the deficient group was 0.97 ± 0.29 ?g LOX / mg protein, while in the animals of the control group it was 1.56 ± 0, 60 ?g LOX /mg protein, being the difference statistically significant (p = 0.03). It is concluded that cattle\nwith copper deficiency secondary to high levels of molybdenum and sulfur in the diet have a decrease activity of the enzyme lysyl oxidase at the cardiac level. The absence of significant morphologic alterations in connective tissue and basement membranes in this work could be associated with the duration of the deficiency. We believe that the data obtained in this thesis will contribute to the knowledge of the pathogenesis suffered by hearts in animals with falling\ndisease. Fil: Nicastro, Carolina Natalia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Buenos Aires, Argentina Postma, Gabriela Minatel, Leonardo Nicastro, Carolina Natalia 2019-11-28 La deficiencia de cobre es una importante enfermedad de los bovinos, ampliamente\ndistribuida a nivel mundial. Esta deficiencia puede ser primaria o secundaria. El cobre es cofactor de numerosas enzimas, por lo cual su deficiencia genera diversas lesiones. Una de\nlas manifestaciones de la deficiencia de cobre en los bovinos en la enfermedad de las caídas, caracterizada por lesiones en el corazón, que ocasionan una insuficiencia cardíaca.\nEl objetivo de esta tesis fue demostrar que existen cambios en la composición microscópica de las membranas basales (MBs) del corazón de bovinos con deficiencia secundaria de\ncobre, inducida por el consumo de dietas con altos niveles de molibdeno y azufre, originadas por una disminución de la actividad de la enzima lisil oxidasa. Se trabajó con 18 muestras de miocardio del ventrículo derecho, provenientes de 2 ensayos experimentales en los que se utilizaron 18 novillos Holando Argentino, de 60 días de edad, divididos en 2 grupos (grupo\ncontrol y grupo deficiente). Las muestras habían sido conservadas en tacos de parafina, de los cuales se obtuvieron cortes de 5 µm para realizar técnicas histoquímicas (picrosirius red) e inmunohistoquímicas (para colágeno tipo IV y laminina). Las muestras congeladas a - 70°C se utilizaron para medir la actividad de la enzima lisil oxidasa (LOX). No se observaron diferencias significativas con la tinción de picrosirius red ni con las técnicas de inmunohistoquímica para colágeno tipo IV y laminina. La concentración de LOX activa en los animales del grupo deficiente fue de 0,97 ± 0,29 µg LOX/ mg proteína, mientras que en los\nanimales del grupo control fue de 1,56 ± 0,60 µg LOX/ mg proteína, siendo esta diferencia estadísticamente significativa (p = 0,03). Se concluye que los bovinos con deficiencia de cobre secundaria a altos niveles molibdeno y azufre en la dieta presentan una menor actividad de la enzima LOX a nivel cardíaco. La ausencia de alteraciones morfológicas significativas en el tejido conectivo y MBs del corazón observadas en este trabajo podría estar asociada a la duración de la deficiencia. Se espera que los datos obtenidos en esta tesis aporten al conocimiento de la patogenia de la enfermedad de las caídas. application/pdf Bovinos Deficiencia de cobre Tejido conectivo Bovine Copper deficiency Tissue connective spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nend/2.5/ Ciencias Veterinarias Magister de la Universidad de Buenos Aires en Salud Animal Efectos de la deficiencia de cobre inducida por altos niveles de molibdeno y azufre en la dieta sobre el tejido conectivo del endomisio y perimisio, y las membranas y perimisio, y las membranas basales del corazón de bovinos info:eu-repo/semantics/masterThesis info:ar-repo/semantics/tesis de maestría info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avemaster&cl=CL1&d=HWA_7020 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avemaster/index/assoc/HWA_7020.dir/7020.PDF |