Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos
HHV-8 genetic diversity shows a worldwide distribution exhibiting a clear ethnic and geographic clustering. Data regarding the prevalence, genetic diversity and evolutionary history of HHV-8 is still scarce in multi-ethnic Latin American populations.\nThe prevalence of HHV-8 was determined in 772 bl...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2020
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7002 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7002.dir/7002.PDF |
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I28-R145-HWA_7002 |
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I28-R145-HWA_70022025-06-26 HHV-8 genetic diversity shows a worldwide distribution exhibiting a clear ethnic and geographic clustering. Data regarding the prevalence, genetic diversity and evolutionary history of HHV-8 is still scarce in multi-ethnic Latin American populations.\nThe prevalence of HHV-8 was determined in 772 blood donors, 101 HIV-1 infected subjects and 93 Kaposi?s Sarcoma patients. ORF-26 and ORF-K1 were analyzed for subtype assignment. A mini-sequencing protocol was designed to identify subtypes. Genetic ancestry was determined by ancestry-sensitive DNA markers from autosomal, Y and mitochondrial chromosomes. Phylogeographic analysis was performed in the ORF-K1.\nHHV-8 DNA was less prevalent among blood donors (p<0.0001), and, among those, viral prevalence was higher among donors from north-western Argentina than from the metropolitan area (p=0.001), Bolivians (p=0.0008), Peruvians and Paraguayans. Analyses of ORF-26 revealed that most strains were subtype A/C, followed by subtypes J, B2, K or R. ORF-K1 analyses revealed the presence of subtypes A, B, C and F. The mini-sequencing technique developed in this thesis was a fast, efficient and cheap to identify the most prevalent subtypes in Argentina. Subtypes A/C and J were significantly associated with Native (p=0.004) and non-Native American maternal haplogroups (p<0.0001), respectively. HHV-8 was introduced from Africa, Europe and Latin America.\nThese results reveal the prevalence and HHV-8 genetic diversity in Argentina and their relationship with human migrations. Fil: Hulaniuk Wolaniuk, María Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Caputo, Mariela Trinks, Julieta Hulaniuk Wolaniuk, María Laura 2020-12-15 La diversidad genética del HHV-8 presenta una distribución mundial con un patrón geográfico y étnico característico. Los datos sobre la prevalencia, diversidad genética e historia evolutiva viral son aún escasos en poblaciones multi-étnicas latinoamericanas.\nSe determinó la prevalencia viral en 772 donantes de sangre, en 101 individuos VIH [+] y en 93 con Sarcoma de Kaposi. Se caracterizaron los subtipos virales y se desarrolló un método de caracterización genotípica basado en mini-secuenciación. Se analizó la ascendencia genética y se realizó un análisis filogeográfico.\nLa prevalencia viral fue menor en los donantes (p<0,0001), entre los cuales, fue mayor en el noroeste argentino en comparación con la región metropolitana (p=0,001), con Bolivia (p=0,0008), Perú y Paarguay. El análisis del ORF-26E reveló la presencia mayoritaria del subtipo A/C seguido de los subtipos J, K o R y B2; mientras que el ORF-K1 demostró la circulación de los subtipos A, B, C y F. La técnica desarrollada resultó ser un método rápido, eficiente y de bajo costo para identificar los subtipos más prevalentes en Argentina. Los subtipos A/C y J se asociaron con haplogrupos maternos nativo-americanos (p=0,004) y no nativo-americanos (p<0,0001), respectivamente. Este virus se introdujo desde África Subsahariana y Europa o de Latinoamérica.\nEstos resultados revelan la prevalencia y circulación del HHV-8 en Argentina y su relación con las migraciones humanas. application/pdf Campos, Rodolfo Pando, María de los Angeles Ré, Viviana HHV-8 spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Doctora de la Universidad de Buenos Aires en Ciencias Biológicas Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7002 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7002.dir/7002.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
HHV-8 |
| spellingShingle |
HHV-8 Hulaniuk Wolaniuk, María Laura Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos |
| topic_facet |
HHV-8 |
| description |
HHV-8 genetic diversity shows a worldwide distribution exhibiting a clear ethnic and geographic clustering. Data regarding the prevalence, genetic diversity and evolutionary history of HHV-8 is still scarce in multi-ethnic Latin American populations.\nThe prevalence of HHV-8 was determined in 772 blood donors, 101 HIV-1 infected subjects and 93 Kaposi?s Sarcoma patients. ORF-26 and ORF-K1 were analyzed for subtype assignment. A mini-sequencing protocol was designed to identify subtypes. Genetic ancestry was determined by ancestry-sensitive DNA markers from autosomal, Y and mitochondrial chromosomes. Phylogeographic analysis was performed in the ORF-K1.\nHHV-8 DNA was less prevalent among blood donors (p<0.0001), and, among those, viral prevalence was higher among donors from north-western Argentina than from the metropolitan area (p=0.001), Bolivians (p=0.0008), Peruvians and Paraguayans. Analyses of ORF-26 revealed that most strains were subtype A/C, followed by subtypes J, B2, K or R. ORF-K1 analyses revealed the presence of subtypes A, B, C and F. The mini-sequencing technique developed in this thesis was a fast, efficient and cheap to identify the most prevalent subtypes in Argentina. Subtypes A/C and J were significantly associated with Native (p=0.004) and non-Native American maternal haplogroups (p<0.0001), respectively. HHV-8 was introduced from Africa, Europe and Latin America.\nThese results reveal the prevalence and HHV-8 genetic diversity in Argentina and their relationship with human migrations. |
| author2 |
Caputo, Mariela |
| author_facet |
Caputo, Mariela Hulaniuk Wolaniuk, María Laura |
| format |
Tesis doctoral Tesis doctoral acceptedVersion |
| author |
Hulaniuk Wolaniuk, María Laura |
| author_sort |
Hulaniuk Wolaniuk, María Laura |
| title |
Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos |
| title_short |
Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos |
| title_full |
Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos |
| title_fullStr |
Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos |
| title_full_unstemmed |
Epidemiología molecular del Herpesvirus humano tipo 8 (HHV-8) en Argentina: asociación con eventos migratorios humanos |
| title_sort |
epidemiología molecular del herpesvirus humano tipo 8 (hhv-8) en argentina: asociación con eventos migratorios humanos |
| publisher |
Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica |
| publishDate |
2020 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7002 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7002.dir/7002.PDF |
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AT hulaniukwolaniukmarialaura epidemiologiamoleculardelherpesvirushumanotipo8hhv8enargentinaasociacionconeventosmigratorioshumanos |
| _version_ |
1840330187206557696 |