Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino

The FSH plays an essential role in cattle reproduction. During the estrous cycle of the cattle, said gonadotropin is responsible for the process of ovarian steroidogenesis, growth and follicular maturation. The FSH acts on the ovarian cells through the union to its specific receptor in the plasma me...

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Autor principal: Amherdt, Leandro A.
Otros Autores: Marelli, Belkis E.
Formato: Tesis de maestría acceptedVersion
Lenguaje:Español
Publicado: Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica 2021
Materias:
FSH
FSHR
Bovinos
Isoformas
Ciencias de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_6876
https://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_6876.dir/6876.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-HWA_6876
record_format dspace
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic FSH
FSHR
Bovinos
Isoformas
Ciencias de la vida
spellingShingle FSH
FSHR
Bovinos
Isoformas
Ciencias de la vida
Amherdt, Leandro A.
Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino
topic_facet FSH
FSHR
Bovinos
Isoformas
Ciencias de la vida
description The FSH plays an essential role in cattle reproduction. During the estrous cycle of the cattle, said gonadotropin is responsible for the process of ovarian steroidogenesis, growth and follicular maturation. The FSH acts on the ovarian cells through the union to its specific receptor in the plasma membrane of the granulosa cells. In cattle, the concept of an only receptor of FSH (FSHR) is nowadays accepted in order to explain the actions of said hormone on the target cells. However, in those gonads from different species such as ships, rats, mice, cattle and human beings, it has been observed that the ARNm which encodes for the complete receptor (full-length receptor) is co-expressed along with different alternative transcript (isoforms). Therefore, the main objective of this thesis consisted of characterizing the expression patterns of isoforms from FSHR in cattle ovaries for a better understanding about the dynamics of follicular development. Based on the obtained results, it was possible to demonstrate that the exons involved in the creation of variants of FSHR include one or more of the exons 5, 6, 9 and 10. Moreover, it was viable to characterize three variants of the receptor in granulosa cells of cattle antral follicles: FSHR-ISO1 (which lacks exons 5 and 6), FSHR-ISO2 (which lacks exon 9), FSHR-ISO3 (which lacks exons 9 and 10). In addition, the presence of a fourth hypothetical isoform was deduced, FSHR-ISO4 (produced from the simultaneous loss of exons 5, 6 and 9). Regarding the behaviour of said variants, it was possible to establish that while the maturation of antral follicle increases, the primary transcript FSHR wt decreases, as well as the isoforms FSH-ISO1 and FSH-ISO2, and the expression of FSHR-ISO3 is kept invariable. Lastly, we can mention that the isoforms FSHR-ISO1, FSHR-ISO2 and FSHR-ISO4 would present structural modifications at receptor extracellular domain level with possible variations in the affinity towards FSH and the receptor activation. In the case of FSHR-ISO3, the lack of transmembrane domain implies the presence of a soluble receptor. The existence of diverse isoforms of FSH in cattle ovaries could be the result of a regulation mechanism aimed at adjusting the effects of FSH on the different ovarian functions. The results of this thesis provide new knowledge to the understanding of the normal ovarian physiology which, besides, could contribute to the development of new biotechnological tools in order to improve the reproductive efficiency in cattle and livestock production in general.
author2 Marelli, Belkis E.
author_facet Marelli, Belkis E.
Amherdt, Leandro A.
format Tesis de maestría
Tesis de maestría
acceptedVersion
author Amherdt, Leandro A.
author_sort Amherdt, Leandro A.
title Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino
title_short Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino
title_full Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino
title_fullStr Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino
title_full_unstemmed Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino
title_sort caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino
publisher Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
publishDate 2021
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_6876
https://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_6876.dir/6876.PDF
work_keys_str_mv AT amherdtleandroa caracterizaciondeisoformasdelreceptordehormonafoliculoestimulanteenelovariobovino
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spelling I28-R145-HWA_68762025-07-31 The FSH plays an essential role in cattle reproduction. During the estrous cycle of the cattle, said gonadotropin is responsible for the process of ovarian steroidogenesis, growth and follicular maturation. The FSH acts on the ovarian cells through the union to its specific receptor in the plasma membrane of the granulosa cells. In cattle, the concept of an only receptor of FSH (FSHR) is nowadays accepted in order to explain the actions of said hormone on the target cells. However, in those gonads from different species such as ships, rats, mice, cattle and human beings, it has been observed that the ARNm which encodes for the complete receptor (full-length receptor) is co-expressed along with different alternative transcript (isoforms). Therefore, the main objective of this thesis consisted of characterizing the expression patterns of isoforms from FSHR in cattle ovaries for a better understanding about the dynamics of follicular development. Based on the obtained results, it was possible to demonstrate that the exons involved in the creation of variants of FSHR include one or more of the exons 5, 6, 9 and 10. Moreover, it was viable to characterize three variants of the receptor in granulosa cells of cattle antral follicles: FSHR-ISO1 (which lacks exons 5 and 6), FSHR-ISO2 (which lacks exon 9), FSHR-ISO3 (which lacks exons 9 and 10). In addition, the presence of a fourth hypothetical isoform was deduced, FSHR-ISO4 (produced from the simultaneous loss of exons 5, 6 and 9). Regarding the behaviour of said variants, it was possible to establish that while the maturation of antral follicle increases, the primary transcript FSHR wt decreases, as well as the isoforms FSH-ISO1 and FSH-ISO2, and the expression of FSHR-ISO3 is kept invariable. Lastly, we can mention that the isoforms FSHR-ISO1, FSHR-ISO2 and FSHR-ISO4 would present structural modifications at receptor extracellular domain level with possible variations in the affinity towards FSH and the receptor activation. In the case of FSHR-ISO3, the lack of transmembrane domain implies the presence of a soluble receptor. The existence of diverse isoforms of FSH in cattle ovaries could be the result of a regulation mechanism aimed at adjusting the effects of FSH on the different ovarian functions. The results of this thesis provide new knowledge to the understanding of the normal ovarian physiology which, besides, could contribute to the development of new biotechnological tools in order to improve the reproductive efficiency in cattle and livestock production in general. Fil: Amherdt, Leandro A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Marelli, Belkis E. Amherdt, Leandro A. 2021-10-06 La FSH desempeña un rol central en la reproducción de los bovinos. Durante el ciclo estral de la vaca, esta gonadotrofina es la responsable del proceso de esteroidogénesis ovárica, crecimiento y maduración folicular. La FSH ejerce sus acciones biológicas sobre las células del ovario mediante la unión a su receptor específico presente en la membrana plasmática de las células de la granulosa. En el bovino, el concepto de un único receptor de FSH (FSHR) es aceptado actualmente para explicar las acciones de dicha hormona sobre las células blanco. No obstante, en las gónadas de diferentes especies como ovejas, ratas, ratones, bovinos y humanos, se ha observado que el ARNm que codifica para el receptor completo (receptor full-length) es co-expresado junto a diferentes transcriptos alternativos (isoformas). Según lo expuesto, el objetivo general de este trabajo de tesis consistió en caracterizar el patrón de expresión de las isoformas del FSHR en ovarios bovinos para una mejor comprensión de la dinámica del desarrollo folicular. A partir de los resultados obtenidos fue posible demostrar que los exones involucrados en la generación de variantes del FSHR incluyen uno o más de los exones 5, 6, 9 y 10. En el mismo sentido, se caracterizaron tres variantes del receptor en células de la granulosa de folículos antrales bovinos: FSHR-ISO1 (la cual carece de los exones 5 y 6), FSHR-ISO2 (la cual carece del exón 9), FSHR-ISO3 (la cual carece de los exones 9 y 10). Además, se infirió la presencia de una cuarta isoforma hipotética, FSHR-ISO4 (generada por la pérdida simultánea de los exones 5, 6 y 9). En cuanto al comportamiento de tales variantes fue posible establecer que, conforme avanza la maduración de los folículos antrales, el transcripto primario FSHR wt disminuye, como así también las isoformas FSH-ISO1 e FHSR-ISO2, mientras se mantiene constante la expresión de FSHR-ISO3. Por último, podemos mencionar que las isoformas FSHR-ISO1, FSHR-ISO2 e FSHR-ISO4, presentarían modificaciones estructurales a nivel del dominio extracelular del receptor con posibles alteraciones en la afinidad hacia FSH y la activación del receptor. En el caso de FSHR-ISO3, la falta del dominio transmembrana hace suponer que se comportaría con un receptor soluble. La presencia de distintas isoformas del FSHR en el ovario bovino podrían ser el resultado de un mecanismo de regulación tendiente a modular los efectos de la FSH sobre las distintas funciones ováricas. Los resultados de este trabajo de tesis aportan nuevos conocimientos al entendimiento de la fisiología ovárica normal que podrían contribuir, además, al desarrollo de nuevas herramientas biotecnológicas destinadas a mejorar la eficiencia reproductiva de los bovinos y la producción pecuaria en general. application/pdf Miragaya, Marcelo Furnus, Cecilila Parborell, Fernanda FSH FSHR Bovinos Isoformas spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Magíster de la Universidad de Buenos Aires en Biología Molecular Médica Caracterización de isoformas del receptor de hormona foliculoestimulante en el ovario bovino info:eu-repo/semantics/masterThesis info:ar-repo/semantics/tesis de maestría info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_6876 https://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_6876.dir/6876.PDF

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