InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN...
The bovine calpastatin (CAST) gene has different isoforms expressed in skeletal muscle that originate from three promoters (I, II and III), with alternative splicing and variable polyadenylation sites. Total and relative expression of isoforms vary between breeds and muscles, contributing to the var...
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| Autores principales: | , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
| Publicado: |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2021
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| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_6769 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_6769.dir/6769.PDF |
| Aporte de: |
| Sumario: | The bovine calpastatin (CAST) gene has different isoforms expressed in skeletal muscle that originate from three promoters (I, II and III), with alternative splicing and variable polyadenylation sites. Total and relative expression of isoforms vary between breeds and muscles, contributing to the variability of the meat tenderness. The objective of this study was to analyze the sequence of the three CAST promoters in Angus (n = 7) and Brahman (n = 7) bulls in order to identify DNA polymorphisms, potential transcription factor binding sites (TFBS) and CpG islands. Thirteen SNPs were identified, four of them would modify potential TFBS in promoter I (NF-?B site: positions 96,034,491, 96,034,660 and 96,034,661 and E2F or Sp1 sites I position 96,034,610 on BTA7; reference genome ARS UCD 1.2) and three in promoter II (in 96,034,941 Sp1 and 96,035,456 and 96,035,504 E2F sites). CpG islands were identified in the three promoters and SNPs in I and II that would alter CpG dinucleotides. These results could lead to new hypotheses and objectives in the investigation of meat quality, particularly about differences among cattle breeds. |
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