InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN...
The bovine calpastatin (CAST) gene has different isoforms expressed in skeletal muscle that originate from three promoters (I, II and III), with alternative splicing and variable polyadenylation sites. Total and relative expression of isoforms vary between breeds and muscles, contributing to the var...
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| Autores principales: | , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2021
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_6769 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_6769.dir/6769.PDF |
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I28-R145-HWA_6769 |
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I28-R145-HWA_67692024-11-05 InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN... The bovine calpastatin (CAST) gene has different isoforms expressed in skeletal muscle that originate from three promoters (I, II and III), with alternative splicing and variable polyadenylation sites. Total and relative expression of isoforms vary between breeds and muscles, contributing to the variability of the meat tenderness. The objective of this study was to analyze the sequence of the three CAST promoters in Angus (n = 7) and Brahman (n = 7) bulls in order to identify DNA polymorphisms, potential transcription factor binding sites (TFBS) and CpG islands. Thirteen SNPs were identified, four of them would modify potential TFBS in promoter I (NF-?B site: positions 96,034,491, 96,034,660 and 96,034,661 and E2F or Sp1 sites I position 96,034,610 on BTA7; reference genome ARS UCD 1.2) and three in promoter II (in 96,034,941 Sp1 and 96,035,456 and 96,035,504 E2F sites). CpG islands were identified in the three promoters and SNPs in I and II that would alter CpG dinucleotides. These results could lead to new hypotheses and objectives in the investigation of meat quality, particularly about differences among cattle breeds. Fil: Motter, M.M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Genética. Buenos Aires, Argentina Fil: Corva, P.M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Departamento de Producción Animal. Buenos Aires, Argentina Fil: Soria, L.A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Genética. Buenos Aires, Argentina Motter, M.M. Corva, P.M. Soria, L.A. 2021 El gen de la calpastatina bovina (CAST) se expresa en músculo esquelético generando diferentes isoformas de ARNm a partir de tres promotores (I, II y III), splicing y poliadenilación alternativos. Su expresión varía entre razas y músculos, lo que es relevante porque contribuye a la variabilidad de la terneza de la carne. El objetivo de este trabajo fue analizar las secuencias de los tres promotores de CAST en toros Angus (n=7) y Brahman (n=7) con el propósito de identificar polimorfismos de ADN, potenciales sitios de unión a factores de transcripción (TFBS o transcription factor binding site) e islas CpG. Se identificaron trece SNP, de los cuales cuatro alterarían potenciales TFBS en el promotor I (sitios NF-?B en las posiciones 96.034.491, 96.034.660 y 96.034.661 de BTA7 del genoma bovino de referencia ARS UCD 1.2 y sitios E2F o Sp1 en la posición 96.034.610) y tres en el promotor II (96.034.941 sitio Sp1 y 96.035.456 y 96.035.504 sitios E2F). Se identificaron islas CpG en los tres promotores y SNPs en I y II que alterarían dinucleótidos CpG. Estos resultados plantean nuevas hipótesis y objetivos para la investigación en la calidad de la carne, y la necesidad de estudios complementarios para validarlos. application/pdf ISSN 1668-3498 (en línea) bovinos para carne calpastatina factores de transcripción islas CpG beef cattle calpastatin transcription factor CpG islands spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 23, nº2 Análisis comparativo de promotores del gen de calpastatina en Angus y Brahman Comparative analysis of promoters of the calpastatin gene in Angus and Brahman info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_6769 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_6769.dir/6769.PDF |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-145 |
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Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
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spa |
| topic |
bovinos para carne calpastatina factores de transcripción islas CpG beef cattle calpastatin transcription factor CpG islands |
| spellingShingle |
bovinos para carne calpastatina factores de transcripción islas CpG beef cattle calpastatin transcription factor CpG islands Motter, M.M. Corva, P.M. Soria, L.A. InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN... |
| topic_facet |
bovinos para carne calpastatina factores de transcripción islas CpG beef cattle calpastatin transcription factor CpG islands |
| description |
The bovine calpastatin (CAST) gene has different isoforms expressed in skeletal muscle that originate from three promoters (I, II and III), with alternative splicing and variable polyadenylation sites. Total and relative expression of isoforms vary between breeds and muscles, contributing to the variability of the meat tenderness. The objective of this study was to analyze the sequence of the three CAST promoters in Angus (n = 7) and Brahman (n = 7) bulls in order to identify DNA polymorphisms, potential transcription factor binding sites (TFBS) and CpG islands. Thirteen SNPs were identified, four of them would modify potential TFBS in promoter I (NF-?B site: positions 96,034,491, 96,034,660 and 96,034,661 and E2F or Sp1 sites I position 96,034,610 on BTA7; reference genome ARS UCD 1.2) and three in promoter II (in 96,034,941 Sp1 and 96,035,456 and 96,035,504 E2F sites). CpG islands were identified in the three promoters and SNPs in I and II that would alter CpG dinucleotides. These results could lead to new hypotheses and objectives in the investigation of meat quality, particularly about differences among cattle breeds. |
| format |
Artículo Artículo publishedVersion |
| author |
Motter, M.M. Corva, P.M. Soria, L.A. |
| author_facet |
Motter, M.M. Corva, P.M. Soria, L.A. |
| author_sort |
Motter, M.M. |
| title |
InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN... |
| title_short |
InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN... |
| title_full |
InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN... |
| title_fullStr |
InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN... |
| title_full_unstemmed |
InVet 2021, 23 (2): 01-10 ARTÍCULO DE INVESTIGACIÓN ISSN... |
| title_sort |
invet 2021, 23 (2): 01-10 artículo de investigación issn... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias |
| publishDate |
2021 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_6769 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_6769.dir/6769.PDF |
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