Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus
The administration of antivenoms based on heterologous polyclonal antibodies\nis the only effective tool available to treat snakebite envenomings and, therefore, is a\npriority for Public Health. Traditionally, these medicines are produced in horses,\nhowever, an alternative to this production is ba...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2021
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_6731 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_6731.dir/6731.PDF |
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I28-R145-HWA_6731 |
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I28-R145-HWA_67312025-06-25 The administration of antivenoms based on heterologous polyclonal antibodies\nis the only effective tool available to treat snakebite envenomings and, therefore, is a\npriority for Public Health. Traditionally, these medicines are produced in horses,\nhowever, an alternative to this production is based on the use of egg yolk antibodies\n(IgY-Technology) due to their economic, ethical and productive advantages. In this\nthesis, IgY-based antivenoms were produced, characterized and evaluated against the\nvenom of Bothrops alternatus. Different variables were evaluated for the\nimmunization of laying hens and the serological response curve was determined by\nELISA. Egg yolk extracts were produced and their protein content was analyzed by\nBradford, ELISA and HPLC-ESI-MS/MS. The antivenoms were able to recognize and\nneutralize up to 68.74 ?g venom/mg antivenom, as well as the hemorrhagic activity of\nthe bothropic venom in a murine model. Likewise, the immunogenicity of the\nantivenom was evaluated. IgY-Technology could enable the production of effective and\naffordable antivenoms that meet the urgent needs of many countries where\nconventional manufacture is unable to provide enough availability of antivenoms. Fil: Leiva, Carlos Leónidas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Fernández-Miyakawa, Mariano Fernández, Marisa Mariel Chacana, Pablo Aníbal Leiva, Carlos Leónidas 2021-03-01 La administración de antivenenos basados en anticuerpos policlonales\nheterólogos es la única herramienta eficaz disponible para tratar los accidentes\nofídicos y, por lo tanto, resulta una prioridad para la salud pública. Tradicionalmente,\nestos medicamentos son producidos en equinos, sin embargo, una alternativa a esta\nproducción se basa en el uso de los anticuerpos de yema de huevo (Tecnología IgY)\ndebido a sus ventajas económicas, éticas y productivas. En esta tesis se produjo,\ncaracterizó y evaluó la eficacia de antivenenos basados en IgY contra el veneno de la\nserpiente Bothrops alternatus. Se evaluaron distintas variables para la inmunización de\ngallinas ponedoras y se determinó la curva de respuesta serológica mediante ELISA. Se\nprodujeron extractos de yema de huevo cuyo contenido de proteínas e IgY fue\nanalizado mediante Bradford, ELISA y HPLC-ESI-MS/MS. Los antivenenos fueron\ncapaces de reconocer y de neutralizar hasta 68,74 ?g de veneno/mg de antiveneno, así\ncomo también la actividad hemorrágica del veneno botrópico en un modelo murino.\nAsimismo, se evaluó la inmunogenicidad del antiveneno. La Tecnología IgY podría\npermitir la producción de antivenenos eficaces y asequibles que satisfagan las\nnecesidades urgentes de muchos países donde la producción convencional es\ndeficiente para asegurar la disponibilidad de antivenenos. application/pdf Maglio, Daniel Gonzalez Zwirner, Norberto Docena, Guillermo Bothrops alternatus Veneno IgY Yema de huevo Neutralización Bothrops alternatus Venom IgY Egg yolk Neutralization spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Doctor de la Universidad de Buenos Aires en Ciencias Bioquímicas Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_6731 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_6731.dir/6731.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Bothrops alternatus Veneno IgY Yema de huevo Neutralización Bothrops alternatus Venom IgY Egg yolk Neutralization Ciencias de la vida |
| spellingShingle |
Bothrops alternatus Veneno IgY Yema de huevo Neutralización Bothrops alternatus Venom IgY Egg yolk Neutralization Ciencias de la vida Leiva, Carlos Leónidas Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus |
| topic_facet |
Bothrops alternatus Veneno IgY Yema de huevo Neutralización Bothrops alternatus Venom IgY Egg yolk Neutralization Ciencias de la vida |
| description |
The administration of antivenoms based on heterologous polyclonal antibodies\nis the only effective tool available to treat snakebite envenomings and, therefore, is a\npriority for Public Health. Traditionally, these medicines are produced in horses,\nhowever, an alternative to this production is based on the use of egg yolk antibodies\n(IgY-Technology) due to their economic, ethical and productive advantages. In this\nthesis, IgY-based antivenoms were produced, characterized and evaluated against the\nvenom of Bothrops alternatus. Different variables were evaluated for the\nimmunization of laying hens and the serological response curve was determined by\nELISA. Egg yolk extracts were produced and their protein content was analyzed by\nBradford, ELISA and HPLC-ESI-MS/MS. The antivenoms were able to recognize and\nneutralize up to 68.74 ?g venom/mg antivenom, as well as the hemorrhagic activity of\nthe bothropic venom in a murine model. Likewise, the immunogenicity of the\nantivenom was evaluated. IgY-Technology could enable the production of effective and\naffordable antivenoms that meet the urgent needs of many countries where\nconventional manufacture is unable to provide enough availability of antivenoms. |
| author2 |
Fernández-Miyakawa, Mariano |
| author_facet |
Fernández-Miyakawa, Mariano Leiva, Carlos Leónidas |
| format |
Tesis doctoral Tesis doctoral acceptedVersion |
| author |
Leiva, Carlos Leónidas |
| author_sort |
Leiva, Carlos Leónidas |
| title |
Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus |
| title_short |
Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus |
| title_full |
Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus |
| title_fullStr |
Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus |
| title_full_unstemmed |
Desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de Bothrops alternatus |
| title_sort |
desarrollo de antivenenos basados en anticuerpos de yema de huevo contra veneno de bothrops alternatus |
| publisher |
Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica |
| publishDate |
2021 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_6731 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_6731.dir/6731.PDF |
| work_keys_str_mv |
AT leivacarlosleonidas desarrollodeantivenenosbasadosenanticuerposdeyemadehuevocontravenenodebothropsalternatus |
| _version_ |
1840330177383497728 |