Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico
Fungi are cosmopolitan organisms, capable of causing diseases that affect human and animal health and crops. Argentina, due to its extension and geographic characteristics, has a great diversity of climates. It is considered as a great source of biological resources linked to the search for new comp...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5766 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5766.dir/5766.PDF |
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I28-R145-HWA_5766 |
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I28-R145-HWA_57662022-04-06 Fungi are cosmopolitan organisms, capable of causing diseases that affect human and animal health and crops. Argentina, due to its extension and geographic characteristics, has a great diversity of climates. It is considered as a great source of biological resources linked to the search for new compounds. The objective of this work was to evaluate the antifungal activity of the methanolic extracts of: Parastrephia quadrangularis, Senna spectabilis var. spectabilis, Peltophorum dubium and Calycophyllum multiflorum, against four phytopathogenic fungi: Fusarium verticillioides and F. gramineraum and Aspergillus parasiticus and A. flavus, as well as knowing their mode of action and to carry out a preliminary phytochemical characterization. First, the Minimum Inhibitory Concentration (MIC) of the methanolic extracts was determined. Then, with the active extracts (MIC<500 ?g/ml) several techniques were performed to demonstrate this activity and the mode of action. Finally, the active extracts were submitted to purification methods and then analyzed by HPLC-UV and MS/MS. For the results obtained, we would be facing active extracts whose activity is fungistatic and also, that the main compound present in the active samples of P. quadrangularis is p-coumaroyl-oxy-tremethone, in the case of S. spectabilis is anthraquinone Emodin, by ther other hand in reference to P. dubium, the majority presence of flavonoids, flavones and flavonols, are detected. Fil: Di Ciaccio, Lucía Soledad. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Salvat, Adriana López, Paula Facultad de Farmacia y Bioquímica Fortunato, Renée H. Di Ciaccio, Lucía Soledad 2018-03-06 Los hongos son organismos ubicuos, capaces de provocar enfermedades que afectan a la salud humana, animal y a los cultivos. Argentina por su extensión y características geográficas, posee gran diversidad de climas, considerándola como una gran fuente de recursos biológicos vinculados a la búsqueda de nuevos compuestos. El objetivo de este trabajo fue evaluar la actividad antifúngica de los extractos metanólicos de: Parastrephia quadrangularis, Senna spectabilis var. spectabilis, Peltophorum dubium y Calycophyllum multiflorum, frente a cuatro hongos fitopatógenos: Fusarium verticillioides y F. gramineraum y Aspergillus parasiticus y A. flavus, asimismo, conocer su modo de acción y realizar una caracterización fitoquímica preliminar. Primero, se determinó la Concentración Mínima Inhibitoria (CMI) de los extractos metanólicos. Luego, con los extractos activos (CMI < 500 ?g/ml) se realizaron diversas técnicas para constatar dicha actividad y el modo de acción. Finalmente, se sometieron a los extractos activos a métodos de purificación analizando las muestras activas por HPLC-UV y MS/MS. Por los resultados obtenidos, estaríamos frente a extractos activos cuya actividad es fungistática y además, que el compuesto principal presente en las muestras activas de P. quadrangularis es p-coumaroil-oxi-tremetona, en el caso de S. spectabilis la antraquinona Emodina, en cambio en referencia a P. dubium se detectó la presencia mayoritaria de flavonoides, del tipo flavonas y flavonoles. application/pdf Broussalis, Adriana Bartoldi, Carmen M. A. Barrera, Viviana Actividad biológica Especies vegetales Flora Norte argentino Argentina spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencias de la vida Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico info:eu-repo/semantics/doctoralThesis info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5766 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5766.dir/5766.PDF |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Actividad biológica Especies vegetales Flora Norte argentino Argentina Ciencias de la vida |
| spellingShingle |
Actividad biológica Especies vegetales Flora Norte argentino Argentina Ciencias de la vida Di Ciaccio, Lucía Soledad Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico |
| topic_facet |
Actividad biológica Especies vegetales Flora Norte argentino Argentina Ciencias de la vida |
| description |
Fungi are cosmopolitan organisms, capable of causing diseases that affect human and animal health and crops. Argentina, due to its extension and geographic characteristics, has a great diversity of climates. It is considered as a great source of biological resources linked to the search for new compounds. The objective of this work was to evaluate the antifungal activity of the methanolic extracts of: Parastrephia quadrangularis, Senna spectabilis var. spectabilis, Peltophorum dubium and Calycophyllum multiflorum, against four phytopathogenic fungi: Fusarium verticillioides and F. gramineraum and Aspergillus parasiticus and A. flavus, as well as knowing their mode of action and to carry out a preliminary phytochemical characterization. First, the Minimum Inhibitory Concentration (MIC) of the methanolic extracts was determined. Then, with the active extracts (MIC<500 ?g/ml) several techniques were performed to demonstrate this activity and the mode of action. Finally, the active extracts were submitted to purification methods and then analyzed by HPLC-UV and MS/MS. For the results obtained, we would be facing active extracts whose activity is fungistatic and also, that the main compound present in the active samples of P. quadrangularis is p-coumaroyl-oxy-tremethone, in the case of S. spectabilis is anthraquinone Emodin, by ther other hand in reference to P. dubium, the majority presence of flavonoids, flavones and flavonols, are detected. |
| author2 |
Salvat, Adriana |
| author_facet |
Salvat, Adriana Di Ciaccio, Lucía Soledad |
| format |
Tesis doctoral Tesis doctoral acceptedVersion |
| author |
Di Ciaccio, Lucía Soledad |
| author_sort |
Di Ciaccio, Lucía Soledad |
| title |
Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico |
| title_short |
Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico |
| title_full |
Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico |
| title_fullStr |
Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico |
| title_full_unstemmed |
Prospección de actividad biológica en representantes de la flora del Norte Argentino con potencial terapéutico |
| title_sort |
prospección de actividad biológica en representantes de la flora del norte argentino con potencial terapéutico |
| publisher |
Facultad de Farmacia y Bioquímica |
| publishDate |
2018 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5766 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5766.dir/5766.PDF |
| work_keys_str_mv |
AT diciaccioluciasoledad prospecciondeactividadbiologicaenrepresentantesdelafloradelnorteargentinoconpotencialterapeutico |
| _version_ |
1766017548131762176 |